Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit B in rats

2009 ◽  
Vol 4 (8) ◽  
pp. 1157-1166 ◽  
Author(s):  
William L Conte ◽  
Hiroaki Kamishina ◽  
Roger L Reep
Keyword(s):  
Cholera Toxin ◽  
Tract Tracing ◽  
Alexa Fluor ◽  
Cholera Toxin Subunit B ◽  
Subunit B

Use of cholera toxin subunit B to label neural projections to lower urinary tract organs v1

2021 ◽  
Author(s):  
Janet R Keast ◽  
Peregrine B Osborne ◽  
John-Paul Fuller-Jackson
Keyword(s):  
Urinary Tract ◽  
Cholera Toxin ◽  
Lower Urinary Tract ◽  
Animal Experimentation ◽  
Female Rat ◽  
Cholera Toxin Subunit B ◽  
Autonomic Neurons ◽  
Subunit B ◽  
Operative Monitoring ◽  
Lower Urinary

This protocol is used to visualise sensory and autonomic neurons innervating organs of the lower urinary tract in an experimental adult male or female rat. The protocol is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation, including methods of anesthesia, surgical environment, and post-operative monitoring and care.

scholarly journals Vestibular Deprivation and the Development of Dendrite Bundles in the Rat

2000 ◽  
Vol 7 (3) ◽  
pp. 193-203 ◽  
Author(s):  
Hildegard C. Geisler ◽  
Jos IJkema-Paassen ◽  
Johan Westerga ◽  
Albert Gramsbergen
Keyword(s):  
Spinal Cord ◽  
Cholera Toxin ◽  
Transverse Direction ◽  
Behavioral Analysis ◽  
Back Muscles ◽  
Cholera Toxin Subunit B ◽  
Postural Tasks ◽  
Dendrite Bundles ◽  
Subunit B

Motoneuronal pools of muscles that subserve postural tasks contain dendrite bundles. We investigated in the rat the development of these bundles in the pools of the long back muscles and related this to postural development. Motoneurons and their dendrites were retrogradely labeled by injecting unconjugated cholera toxin subunit B (CTB) into the muscles of 54 normal rats from birth until adulthood and into 18 rats that were vestibularly deprived from the 5th postnatal day (P5). Dendrite bundles coursing in a transverse direction already occurred at P1. From P4, the first longitudinal bundles could be observed, but the major spurt in development occurred between P6 and P9, when conspicuous bundles developed coursing in rostro-caudal and tranverse directions. This is the age when rats become able to stand freely and walk a few steps. Around P20, the dendrite bundles attained their adult characteristics. Vestibular deprivation by plugging both semicircular horizontal canals did not lead to a retarded development of dendrite bundles nor to a changed morphology. This finding is remarkable, as behavioral analysis showed a delay in postural development by about 3 days. We hypothesize that dendrite bundles in the pools of the long back muscles function to synchronize the motoneurons in different spinal cord segments.

scholarly journals Hetero-multivalent binding of cholera toxin subunit B with glycolipid mixtures

2017 ◽  
Vol 160 ◽  
pp. 281-288 ◽  
Author(s):  
Pratik Krishnan ◽  
Akshi Singla ◽  
Chin-An Lee ◽  
Joshua D. Weatherston ◽  
Nolan C. Worstell ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin Subunit B ◽  
Multivalent Binding ◽  
Subunit B
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