Synchronous culture of Plasmodium falciparum at high parasitemia levels

2009 ◽  
Vol 4 (12) ◽  
pp. 1899-1915 ◽  
Author(s):  
Azar Radfar ◽  
Darío Méndez ◽  
Carlos Moneriz ◽  
María Linares ◽  
Patricia Marín-García ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Synchronous Culture ◽  
High Parasitemia

scholarly journals Improved Protocol for Continuous Culture of Plasmodium falciparum Reference Strains

2021 ◽  
Vol 15 (1) ◽  
pp. 123-129
Author(s):  
Tano Konan Dominique ◽  
Koffi Akissi Jeanne ◽  
Dablé Marius Tresor ◽  
Silué Kigbafori Dieudonné ◽  
Tuo Karim ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Continuous Culture ◽  
Research Topics ◽  
Antimalarial Resistance ◽  
Administrative Procedures ◽  
High Level ◽  
Validation Of Results ◽  
Reference Strains ◽  
High Parasitemia

Parasitic biobank of Plasmodium falciparum is almost germinal in Côte d’Ivoire. However, several high-level research topics on this parasite involve the taking into account of nature isolates but also chemo-sensitive or resistant reference strains for a better validation of results. In addition, acquisition of these reference strains is still arduous for laboratories in developing countries due to complexity of administrative procedures. For those reasons, this study aimed in to combine several procedures into a consolidated one in order to enhance the multiplication of P. falciparum reference strains. Continuous culture of plasmodial strains was based on the Trager and Jensen procedures. The CELL culture protocols used are those of the Swiss TPH described by Sergio Wittlin; the “Growing Plasmodium falciparum cultures at high parasitemia” and the “Stockholm sorbitol method” of Methods in Malaria Research-6th edition 2013; and the INV-01 and INV-02 procedures of the Worldwide Antimalarial Resistance Network (WWARN). Reference Plasmodium falciparum strains NF54 sensitive to chloroquine (CQs) and K1 resistant to chloroquine (CQr) were received from the Swiss Tropical Institute and Public Health (Swiss TPH). The CQs NF54 strain reacted more quickly to the protocol unlike the CQr K1 strain. Parasitic densities (DP) obtained with NF54 strain were ranged from 0.4% at day zero (D0) to 11.4% at day eight (D8). Strain K1 finally adapted successfully after one month of follow-up. Related DPs ranged from less than 0.1% to more than 20% in just three growth cycles after adaptation. A joint protocol (from this work) called “CRLP-SwissTPH-Pasteur_001” is available and allows to efficiently multiply reference strains NF54 and K1. It is planned to spread out the tests to other plasmodial strains as well as to wild isolates in order to standardize this procedure.

Evaluating the dual reactivity on SD bioline malaria rapid diagnosis tests as a potential indicator of high parasitemia due to Plasmodium falciparum

2021 ◽  
pp. 1-9
Author(s):  
Roman Rodrigue Dongang Nana ◽  
Valerie Makoge ◽  
Ngum Lesley Ngum ◽  
Nathalie Amvongo-Adjia ◽  
Vineeta Singh ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Rapid Diagnosis ◽  
Dual Reactivity ◽  
Potential Indicator ◽  
High Parasitemia

scholarly journals Association between Plasmodium falciparum parasite index and renal function in children

2016 ◽  
Vol 43 (3) ◽  
pp. 91
Author(s):  
A Umboh ◽  
S Muljanto ◽  
J S Wibisono
Keyword(s):  
Plasmodium Falciparum ◽  
Renal Function ◽  
Falciparum Malaria ◽  
Field Data ◽  
Filtration Rate ◽  
Significant Elevation ◽  
Chi Square ◽  
Plasmodium Falciparum Parasite ◽  
Falciparum Parasite ◽  
High Parasitemia

Background Falciparum malaria is an acute systemic diseasethat can cause multi organ disorders including in kidney. The aimof this study was to figure out the association between P.falciparumparasite index and renal function in children by measuring ureum,creatinine, and glomerular filtration rate (GFR).Methods An analytic prospective study was conducted on all hos-pitalized falciparum malaria patients in the Department of ChildHealth, Manado Central General Hospital from November 2001-February 2002. Low parasitemia was defined if P.falciparum foundwas ≤100/100 fields and high parasitemia if >1/1 field. Data wereanalyzed by chi-square and student t–test.Results Among 34 patients, there were 11 with high parasitemiaand 23 with low parasitemia. There were 19 with increased ureumand 8 with increased creatinine. There was significant elevation ofureum in high parasitemia (n=10) compared to that in low para-sitemia patients (n=9) with p=0.004. The increase of serum creati-nine was found in 2 high parasitemia and in 6 low parasitemiapatients (p=0.611). Mean GFR was 107.63±30.62 in high and121.94±44.06 in low parasitemia patients (p=0.341).Conclusion There was a significant association betweenP.falciparum parasite index and serum ureum. But there was nosignificant association between parasite index and creatinine orGFR

In Vitro Cultivation of Plasmodium Falciparum at High Parasitemia *

1974 ◽  
Vol 23 (6) ◽  
pp. 1015-1018 ◽  
Author(s):  
Wasim A. Siddiqui ◽  
Suzanne Richmond-Crum ◽  
Jerome V. Schnell
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Cultivation ◽  
High Parasitemia

Impaired cell-mediated immunity in Plasmodium falciparum-infected patients with high-parasitemia and cerebral malaria

1983 ◽  
Vol 27 (1) ◽  
pp. 38-50 ◽  
Author(s):  
Ph. Brasseur ◽  
M. Agrapart ◽  
J.J. Ballet ◽  
P. Druilhe ◽  
M.J. Warrell ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cerebral Malaria ◽  
Cell Mediated Immunity ◽  
High Parasitemia

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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