Determination of gene expression patterns using in situ hybridization to Drosophila testes

2009 ◽  
Vol 4 (12) ◽  
pp. 1807-1819 ◽  
Author(s):  
Ceri A Morris ◽  
Elizabeth Benson ◽  
Helen White-Cooper
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Gene Expression Patterns

scholarly journals RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Mujeeb Shittu ◽  
Tessa Steenwinkel ◽  
William Dion ◽  
Nathan Ostlund ◽  
Komal Raja ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Drosophila Species ◽  
Gene Expression Patterns ◽  
Probe Design ◽  
Tissue Preparation ◽  
Design And Synthesis ◽  
Rna In Situ Hybridization

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.

ZebraFISH: Fluorescent In Situ Hybridization Protocol and Three-Dimensional Imaging of Gene Expression Patterns

Zebrafish ◽  
2006 ◽  
Vol 3 (4) ◽  
pp. 465-476 ◽  
Author(s):  
Monique C.M. Welten ◽  
Simon B. de Haan ◽  
Niels van den Boogert ◽  
Jasprien N. Noordermeer ◽  
Gerda E.M. Lamers ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Expression Patterns ◽  
Three Dimensional ◽  
Gene Expression Patterns ◽  
Three Dimensional Imaging ◽  
Hybridization Protocol

scholarly journals Extraction and comparison of gene expression patterns from 2D RNA in situ hybridization images

2009 ◽  
Vol 26 (6) ◽  
pp. 761-769 ◽  
Author(s):  
Daniel L. Mace ◽  
Nicole Varnado ◽  
Weiping Zhang ◽  
Erwin Frise ◽  
Uwe Ohler
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Rna In Situ Hybridization
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