Accurate measurement of cytosolic free zinc using eCALWY variants: a toolbox of genetically encoded FRET sensors

2009 ◽  
Author(s):  
Jan L. Vinkenborg ◽  
Elisa A. Bellomo ◽  
Tamara J. Nicolson ◽  
Melissa S. Koay ◽  
Maarten Merkx ◽  
...  
Keyword(s):  
2021 ◽  
Vol 9 (11) ◽  
pp. 7025-7033
Author(s):  
Hai-Yang Wu ◽  
Xingxing Gu ◽  
Peng Huang ◽  
Chuang Sun ◽  
Hai Hu ◽  
...  

Dendrite-free Zn anodes are achieved by using the highly efficient electrolyte additives based on polyoxometalate. Both of the anions and cations of polyoxometalate play important roles in inhibiting the growth Zn dendrites.


2021 ◽  
Vol 594 ◽  
pp. 389-397
Author(s):  
Anran Wang ◽  
Weijun Zhou ◽  
Minfeng Chen ◽  
Aixiang Huang ◽  
Qinghua Tian ◽  
...  

2020 ◽  
Vol 27 ◽  
pp. 109-116 ◽  
Author(s):  
Yan Tang ◽  
Cunxin Liu ◽  
Hanrui Zhu ◽  
Xuesong Xie ◽  
Jiawei Gao ◽  
...  

2021 ◽  
pp. 139094
Author(s):  
Yachun Mao ◽  
Huaizheng Ren ◽  
Jiachi Zhang ◽  
Tao Luo ◽  
Nannan Liu ◽  
...  

Nano Letters ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 876-882 ◽  
Author(s):  
Zhi Zhang ◽  
Kun Zheng ◽  
Zhen-Yu Lu ◽  
Ping-Ping Chen ◽  
Wei Lu ◽  
...  
Keyword(s):  

2004 ◽  
Vol 380 (3) ◽  
pp. 695-703 ◽  
Author(s):  
Yongyi BI ◽  
Richard D. PALMITER ◽  
Kristi M. WOOD ◽  
Qiang MA

Phenolic antioxidants, such as tBHQ [2,5-di-(t-butyl)-1,4-hydroquinone], induce Mt1 (metallothionein 1) gene expression and accumulation of MT protein. Induction of Mt1 mRNA does not depend on protein synthesis, and correlates with oxidation–reduction functions of the antioxidants. In the present study, we analysed the biochemical pathway of the induction. Induction depends on the presence of MTF-1 (metal-activated transcription factor 1), a transcription factor that is required for metal-induced transcription of Mt1, but does not require nuclear factor erythroid 2-related factor 2, a tBHQ-activated CNC bZip (cap ‘n’ collar basic leucine zipper) protein, that is responsible for regulating genes encoding phase II drug-metabolizing enzymes. Moreover, tBHQ induces the expression of MRE-βGeo, a reporter gene driven by five metal response elements that constitute an optimal MTF-1 binding site. Reconstitution of Mtf1-null cells with MTF-1 restores induction by both zinc and tBHQ. Unlike activation of phase II genes by tBHQ, induction of Mt1 expression does not occur in the presence of EDTA, when cells are cultured in zinc-depleted medium, or in cells with reduced intracellular ‘free’ zinc due to overexpression of ZnT1, a zinc-efflux transporter, indicating that induction requires zinc. In addition, fluorescence imaging reveals that tBHQ increases cytoplasmic free zinc concentration by mobilizing intracellular zinc pools. These findings establish that phenolic antioxidants activate Mt1 transcription by a zinc-dependent mechanism, which involves MTF-1 binding to metal regulator elements in the Mt1 gene promoter.


2001 ◽  
Vol 79 (38) ◽  
pp. 53
Author(s):  
MAUREEN ROUHI
Keyword(s):  

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