Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniques

2009 ◽  
Vol 4 (11) ◽  
pp. 1653-1662 ◽  
Author(s):  
Jen-Hsuan Wei ◽  
Joachim Seemann
Keyword(s):  
Cell Division ◽  
Correlative Microscopy ◽  
Microscopy Techniques ◽  
Asymmetrical Cell Division

Three dimensional imaging and analysis of a single nano-device at the ultimate scale using correlative microscopy techniques

2015 ◽  
Vol 106 (21) ◽  
pp. 213102 ◽  
Author(s):  
A. Grenier ◽  
S. Duguay ◽  
J. P. Barnes ◽  
R. Serra ◽  
N. Rolland ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Correlative Microscopy ◽  
Three Dimensional Imaging ◽  
Microscopy Techniques

scholarly journals Synthetic pluripotent bacterial stem cells

2018 ◽  
Author(s):  
Sara Molinari ◽  
David L. Shis ◽  
James Chappell ◽  
Oleg A. Igoshin ◽  
Matthew R. Bennett
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Plasmid Dna ◽  
Daughter Cell ◽  
Asymmetric Cell Division ◽  
Genetic Circuit ◽  
Pluripotent Cells ◽  
Daughter Cells ◽  
Asymmetric Partitioning ◽  
Asymmetrical Cell Division

AbstractA defining property of stem cells is their ability to differentiate via asymmetric cell division, in which a stem cell creates a differentiated daughter cell but retains its own phenotype. Here, we describe a synthetic genetic circuit for controlling asymmetrical cell division in Escherichia coli. Specifically, we engineered an inducible system that can bind and segregate plasmid DNA to a single position in the cell. Upon division, the co-localized plasmids are kept by one and only one of the daughter cells. The other daughter cell receives no plasmid DNA and is hence irreversibly differentiated from its sibling. In this way, we achieved asymmetric cell division though asymmetric plasmid partitioning. We also characterized an orthogonal inducible circuit that enables the simultaneous asymmetric partitioning of two plasmid species – resulting in pluripotent cells that have four distinct differentiated states. These results point the way towards engineering multicellular systems from prokaryotic hosts.

Preliminary study on dinosaur rib microstructure by applying correlative microscopy techniques

2011 ◽  
Vol 15 (3) ◽  
pp. 225-235 ◽  
Author(s):  
Jung-Kyun Kim ◽  
Min Huh ◽  
Seon-Gyu Lee ◽  
Youn-Joong Kim
Keyword(s):  
Correlative Microscopy ◽  
Preliminary Study ◽  
Microscopy Techniques

Correlating Atom Probe Crystallographic Measurements with Transmission Kikuchi Diffraction Data

2017 ◽  
Vol 23 (2) ◽  
pp. 279-290 ◽  
Author(s):  
Andrew J. Breen ◽  
Katharina Babinsky ◽  
Alec C. Day ◽  
K. Eder ◽  
Connor J. Oakman ◽  
...  
Keyword(s):  
Atom Probe Tomography ◽  
Angular Resolution ◽  
Atom Probe ◽  
Correlative Microscopy ◽  
Alloy Specimen ◽  
Experimental Conditions ◽  
Research Problems ◽  
Engineering Alloys ◽  
Microscopy Techniques ◽  
First Time

AbstractCorrelative microscopy approaches offer synergistic solutions to many research problems. One such combination, that has been studied in limited detail, is the use of atom probe tomography (APT) and transmission Kikuchi diffraction (TKD) on the same tip specimen. By combining these two powerful microscopy techniques, the microstructure of important engineering alloys can be studied in greater detail. For the first time, the accuracy of crystallographic measurements made using APT will be independently verified using TKD. Experimental data from two atom probe tips, one a nanocrystalline Al–0.5Ag alloy specimen collected on a straight flight-path atom probe and the other a high purity Mo specimen collected on a reflectron-fitted instrument, will be compared. We find that the average minimum misorientation angle, calculated from calibrated atom probe reconstructions with two different pole combinations, deviate 0.7° and 1.4°, respectively, from the TKD results. The type of atom probe and experimental conditions appear to have some impact on this accuracy and the reconstruction and measurement procedures are likely to contribute further to degradation in angular resolution. The challenges and implications of this correlative approach will also be discussed.

scholarly journals SmdA is a novel cell morphology determinant in Staphylococcus aureus

2021 ◽  
Author(s):  
Ine Storaker Myrbråten ◽  
Gro A. Stamsås ◽  
Helena Chan ◽  
Danae Morales Angeles ◽  
Tiril Mathiesen Knutsen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Cell Division ◽  
Cell Morphology ◽  
Spherical Shape ◽  
Major Influence ◽  
Cell Wall Synthesis ◽  
Division Plane ◽  
Microscopy Techniques ◽  
Two Hybrid

Cell division and cell wall synthesis in staphylococci need to be precisely coordinated and controlled to allow the cell to multiply while maintaining their nearly spherical shape. The mechanisms ensuring correct placement of the division plane and synthesis of new cell wall have been studied intensively, however, hitherto unknown factors and proteins are likely to play key roles in this complex interplay. Starting from a subcellular localization- and gene knockdown screen of essential genes with unknown functions in Staphylococcus aureus, we identified a protein with major influence on cell morphology in S. aureus. The protein, here named SmdA (for staphylococcal morphology determinant A), is a membrane-protein with septum-enriched localization. By smdA silencing and overexpression, we have used different microscopy techniques to show that SmdA is critical for cell division, including septum formation and cell splitting. We also identified conserved residues in SmdA that are critical for functionality. Pulldown- and bacterial two-hybrid interaction experiments showed that SmdA interacts with several known cell division- and cell wall synthesis proteins, including penicillin binding proteins (PBPs) and EzrA. Notably, SmdA also affects susceptibility to cell wall targeting antibiotics, particularly in methicillin-resistant S. aureus (MRSA). Together, our results show that S. aureus is dependent on balanced amounts of membrane-attached SmdA in order to carry out proper cell division.

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