The adhesive removal test: a sensitive method to assess sensorimotor deficits in mice

2009 ◽  
Vol 4 (10) ◽  
pp. 1560-1564 ◽  
Author(s):  
Valentine Bouet ◽  
Michel Boulouard ◽  
Jérôme Toutain ◽  
Didier Divoux ◽  
Myriam Bernaudin ◽  
...  
Keyword(s):  
Sensorimotor Deficits ◽  
Adhesive Removal ◽  
Sensitive Method

scholarly journals Lickometry: A novel and sensitive method for assessing functional deficits in rats after stroke

2017 ◽  
Vol 37 (3) ◽  
pp. 755-761 ◽  
Author(s):  
Jewel Ahmed ◽  
Dominic M Dwyer ◽  
Tracy D Farr ◽  
David J Harrison ◽  
Stephen B Dunnett ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Artery Occlusion ◽  
Precise Method ◽  
Functional Deficits ◽  
Sensorimotor Deficits ◽  
Stroke Research ◽  
Cerebral Artery Occlusion ◽  
Sensitive Method

The need for sensitive, easy to administer assessments of long-term functional deficits is crucial in pre-clinical stroke research. In the present study, we introduce lickometry (lick microstructure analysis) as a precise method to assess sensorimotor deficits up to 40 days after middle cerebral artery occlusion in rats. Impairments in drinking efficiency compared to controls, and a compensatory increase in the number of drinking clusters were observed. This highlights the utility of this easy to administer task in assessing subtle, long-term deficits, which could be likened to oral deficits in patients.

Relationship Between Total Prothrombin, Native Prothrombin and the International Normalized Ratio (INR)

1992 ◽  
Vol 68 (02) ◽  
pp. 160-164 ◽  
Author(s):  
P J Braun ◽  
K M Szewczyk
Keyword(s):  
Anticoagulant Therapy ◽  
Oral Anticoagulant ◽  
Inverse Relationship ◽  
Low Dose ◽  
Oral Anticoagulant Therapy ◽  
International Normalized Ratio ◽  
Antigen Assay ◽  
Dose Oral ◽  
Anticoagulated Patients ◽  
Sensitive Method

SummaryPlasma levels of total prothrombin and fully-carboxylated (native) prothrombin were compared with results of prothrombin time (PT) assays for patients undergoing oral anticoagulant therapy. Mean concentrations of total and native prothrombin in non-anticoagulated patients were 119 ± 13 µg/ml and 118 ± 22 µg/ml, respectively. In anticoagulated patients, INR values ranged as high as 9, and levels of total prothrombin and native prothrombin decreased with increasing INR to minimum values of 40 µg/ml and 5 µg/ml, respectively. Des-carboxy-prothrombin increased with INR, to a maximum of 60 µg/ml. The strongest correlation was observed between native prothrombin and the reciprocal of the INR (1/INR) (r = 0.89, slope = 122 µg/ml, n = 200). These results indicated that native prothrombin varied over a wider range and was more closely related to INR values than either total or des-carboxy-prothrombin. Levels of native prothrombin were decreased 2-fold from normal levels at INR = 2, indicating that the native prothrombin antigen assay may be a sensitive method for monitoring low-dose oral anticoagulant therapy. The inverse relationship between concentration of native prothrombin and INR may help in identification of appropriate therapeutic ranges for oral anticoagulant therapy.

Microangiography - A Sensitive Method for Studying Experimental Diabetic Nephropathy

1993 ◽  
Vol 34 (4) ◽  
pp. 376-380 ◽  
Author(s):  
J. Merikanto ◽  
S. -O. Hietala ◽  
F. Lithner ◽  
E. Hagg ◽  
M. Paivansalo
Keyword(s):  
Diabetic Nephropathy ◽  
Sensitive Method

Food Safety: Green, Cost-Effective And Sensitive Method To Detect Metronidazole And Other 5-NDZ Residues In Food

2018 ◽  
Author(s):  
Maykel Hernández-Mesa ◽  
David Moreno-González ◽  
Carmen Cruces-Blanco ◽  
Ana M. García-Campaña
Keyword(s):  
Food Safety ◽  
Cost Effective ◽  
Sensitive Method

Implicit-Flow-Sensitive Method for Detection of Trojan-Spy Programs

2010 ◽  
Vol 21 (6) ◽  
pp. 1426-1437
Author(s):  
Jia-Jing LI ◽  
Zhi-Yin LIANG ◽  
Tao WEI ◽  
Wei ZOU ◽  
Jian MAO
Keyword(s):  
Sensitive Method

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

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