Neutral red uptake assay for the estimation of cell viability/cytotoxicity

2008 ◽  
Vol 3 (7) ◽  
pp. 1125-1131 ◽  
Author(s):  
Guillermo Repetto ◽  
Ana del Peso ◽  
Jorge L Zurita
Keyword(s):  
Cell Viability ◽  
Neutral Red ◽  
Uptake Assay ◽  
Neutral Red Uptake ◽  
Neutral Red Uptake Assay

scholarly journals Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay

2020 ◽  
Vol 3 (10) ◽  
pp. 6633-6638
Author(s):  
Brian J. Nablo ◽  
Jung-Joon Ahn ◽  
Kiran Bhadriraju ◽  
Jong Muk Lee ◽  
Darwin R. Reyes
Keyword(s):  
Cell Viability ◽  
Neutral Red ◽  
Dynamic Cell ◽  
Uptake Assay ◽  
Neutral Red Uptake ◽  
Neutral Red Uptake Assay

scholarly journals Antiangiogenic Activity and Cytotoxicity of Triterpenoids and Homoisoflavonoids from Massonia pustulata and Massonia bifolia

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 638-644 ◽  
Author(s):  
Sianne Schwikkard ◽  
Hannah Whitmore ◽  
Timothy Corson ◽  
Kamakshi Sishtla ◽  
Moses Langat ◽  
...  
Keyword(s):  
Far East ◽  
Neutral Red ◽  
Tube Formation ◽  
Microvascular Endothelial Cells ◽  
Antiangiogenic Activity ◽  
Endothelial Tube Formation ◽  
Uptake Assay ◽  
Neutral Red Uptake ◽  
Dose Dependent ◽  
Neutral Red Uptake Assay

AbstractThe Hyacinthaceae family (sensu APGII), with approximately 900 species in around 70 genera, plays a significant role in traditional medicine in Africa as well as across Europe and the Middle and Far East. The dichloromethane extract of the bulbs of Massonia pustulata (Hyacinthaceae sensu APGII) yielded two known homoisoflavonoids, (R)-5-hydroxy-3-(4-hydroxybenzyl)-7-methoxy-4-chromanone 1 and 5-hydroxy-3-(4-hydroxybenzyl)-7-methoxy-4-chromone 2 and four spirocyclic nortriterpenoids, eucosterol 3, 28-hydroxyeucosterol 4 and two previously unreported triterpenoid derivatives, (17S,23S)-17α,23-epoxy-3β,22β,29-trihydroxylanost-8-en-27,23-olide 5, and (17S, 23S)-17α,23-epoxy-28,29-dihydroxylanost-8-en-3-on-27,23-olide 6. Compounds 1, 2, 3, and 5 were assessed for cytotoxicity against CaCo-2 cells using a neutral red uptake assay. Compounds 1, 2, and 5 reduced cell viability by 70% at concentrations of 30, 100, and 100 µM, respectively. Massonia bifolia yielded three known homoisoflavonoids, (R)-(4′-hydroxy)-5-hydroxy-7-methoxy-4-chromanone 1, (R)-(4′-hydroxy)-5,7-dihydroxy-4-chromanone 7 and (R)-(3′-hydroxy-4′-methoxy)-5,7-dihydroxy-4-chromanone 9, two previously unreported homoisoflavonoids, (E)-3-benzylidene-(3′,4′-dihydroxy)-5-hydroxy-7-methoxy-4-chromanone 8 and (R)-(3′,4′-dihydroxy)-5-hydroxy-7-methoxy-4-chromanone 10, and a spirocyclic nortriterpenoid, 15-deoxoeucosterol 11. Compounds 1, 1Ac, 7, 8, 9, and 10 were screened for antiangiogenic activity against human retinal microvascular endothelial cells. Some compounds showed dose-dependent antiproliferative activity and blocked endothelial tube formation, suggestive of antiangiogenic activity.

Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay

2006 ◽  
Vol 20 (4) ◽  
pp. 480-489 ◽  
Author(s):  
Dijoux Nathalie ◽  
Guingand Yannick ◽  
Bourgeois Caroline ◽  
Durand Sandrine ◽  
Fromageot Claude ◽  
...  
Keyword(s):  
Essential Oils ◽  
Neutral Red ◽  
Uptake Assay ◽  
Neutral Red Uptake ◽  
Neutral Red Uptake Assay
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