scholarly journals Electrical stimulation systems for cardiac tissue engineering

2009 ◽  
Vol 4 (2) ◽  
pp. 155-173 ◽  
Author(s):  
Nina Tandon ◽  
Christopher Cannizzaro ◽  
Pen-Hsiu Grace Chao ◽  
Robert Maidhof ◽  
Anna Marsano ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

scholarly journals Optimization of electrical stimulation parameters for cardiac tissue engineering

2011 ◽  
Vol 5 (6) ◽  
pp. e115-e125 ◽  
Author(s):  
Nina Tandon ◽  
Anna Marsano ◽  
Robert Maidhof ◽  
Leo Wan ◽  
Hyoungshin Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Stimulation Parameters

scholarly journals Cardiac tissue engineering

2005 ◽  
Vol 70 (3) ◽  
pp. 541-556 ◽  
Author(s):  
Milica Radisic ◽  
Gordana Vunjak-Novakovic
Keyword(s):  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Peripheral Region ◽  
Heart Cells ◽  
Coupled Cells ◽  
Continuous Application ◽  
Rat Heart Cells

We hypothesized that clinically sized (1-5 mm thick),compact cardiac constructs containing physiologically high density of viable cells (?108 cells/cm3) can be engineered in vitro by using biomimetic culture systems capable of providing oxygen transport and electrical stimulation, designed to mimic those in native heart. This hypothesis was tested by culturing rat heart cells on polymer scaffolds, either with perfusion of culture medium (physiologic interstitial velocity, supplementation of per fluorocarbons), or with electrical stimulation (continuous application of biphasic pulses, 2 ms, 5 V, 1 Hz). Tissue constructs cultured without perfusion or electrical stimulation served as controls. Medium perfusion and addition of per fluorocarbons resulted in compact, thick constructs containing physiologic density of viable, electromechanically coupled cells, in contrast to control constructs which had only a?100 ?m thick peripheral region with functionally connected cells. Electrical stimulation of cultured constructs resulted in markedly improved contractile properties, increased amounts of cardiac proteins, and remarkably well developed ultrastructure (similar to that of native heart) as compared to non-stimulated controls. We discuss here the state of the art of cardiac tissue engineering, in light of the biomimetic approach that reproduces in vitro some of the conditions present during normal tissue development.

Practical Aspects of Cardiac Tissue Engineering With Electrical Stimulation

2007 ◽  
pp. 291-307 ◽  
Author(s):  
Christopher Cannizzaro ◽  
Nina Tandon ◽  
Elisa Figallo ◽  
Hyoungshin Park ◽  
Sharon Gerecht ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

scholarly journals Extrinsically Conductive Nanomaterials for Cardiac Tissue Engineering Applications

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 914
Author(s):  
Arsalan Ul Haq ◽  
Felicia Carotenuto ◽  
Paolo Di Nardo ◽  
Roberto Francini ◽  
Paolo Prosposito ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Scar Tissue ◽  
Cardiac Tissue Engineering ◽  
Assistive Device ◽  
Long Run ◽  
Fibrotic Scar ◽  
Regeneration Capability ◽  
Conductive Nanomaterials

Myocardial infarction (MI) is the consequence of coronary artery thrombosis resulting in ischemia and necrosis of the myocardium. As a result, billions of contractile cardiomyocytes are lost with poor innate regeneration capability. This degenerated tissue is replaced by collagen-rich fibrotic scar tissue as the usual body response to quickly repair the injury. The non-conductive nature of this tissue results in arrhythmias and asynchronous beating leading to total heart failure in the long run due to ventricular remodelling. Traditional pharmacological and assistive device approaches have failed to meet the utmost need for tissue regeneration to repair MI injuries. Engineered heart tissues (EHTs) seem promising alternatives, but their non-conductive nature could not resolve problems such as arrhythmias and asynchronous beating for long term in-vivo applications. The ability of nanotechnology to mimic the nano-bioarchitecture of the extracellular matrix and the potential of cardiac tissue engineering to engineer heart-like tissues makes it a unique combination to develop conductive constructs. Biomaterials blended with conductive nanomaterials could yield conductive constructs (referred to as extrinsically conductive). These cell-laden conductive constructs can alleviate cardiac functions when implanted in-vivo. A succinct review of the most promising applications of nanomaterials in cardiac tissue engineering to repair MI injuries is presented with a focus on extrinsically conductive nanomaterials.

scholarly journals Designing of spider silk proteins for hiPSC-based cardiac tissue engineering

2021 ◽  
pp. 100114
Author(s):  
Tilman U. Esser ◽  
Vanessa T. Trossmann ◽  
Sarah Lentz ◽  
Felix B. Engel ◽  
Thomas Scheibel
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Proteins
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