Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells

2007 ◽  
Vol 2 (10) ◽  
pp. 2348-2353 ◽  
Author(s):  
Yansong Miao ◽  
Liwen Jiang
Keyword(s):  
Transient Expression ◽  
Fusion Proteins ◽  
Cultured Cells ◽  
Suspension Cultured Cells

Induction of freezing tolerance in potato (Solanum commersonii) suspension cultured cells

1992 ◽  
Vol 84 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Stephen P. Lee ◽  
Baolong Zhu ◽  
Tony H. H. Chen ◽  
Paul H. Li
Keyword(s):  
Freezing Tolerance ◽  
Cultured Cells ◽  
Solanum Commersonii ◽  
Suspension Cultured Cells

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

Direct protein delivery into intact plant cells using polyhistidine peptides

2021 ◽  
Author(s):  
Yoshino Tanaka ◽  
Yoshihiko Nanasato ◽  
Kousei Omura ◽  
Keita Endoh ◽  
Tsuyoshi Kawano ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fusion Proteins ◽  
Protein Delivery ◽  
Fluorescent Protein ◽  
Cultured Cells ◽  
Plant Cells ◽  
Adenosine Monophosphate ◽  
Cell Penetrating Peptides ◽  
Intracellular Space ◽  
Intracellular Fluorescence

Abstract Polyhistidine peptides (PHPs), sequences comprising only histidine residues (>His8), are effective cell-penetrating peptides for plant cells. Using PHP-fusion proteins, we aimed to deliver proteins into cultured plant cells from Nicotiana tabacum, Oryza sativa, and Cryptomeria japonica. Co-cultivation of cultured cells with fusion proteins combining maltose-binding protein (MBP), red fluorescent protein (RFP), and various PHPs (MBP-RFP-His8–His20) in one polypeptide showed the cellular uptake of fusion proteins in all plant cell lines. Maximum intracellular fluorescence was shown in MBP-RFP-His20. Further, adenylate cyclase (CyaA), a synthase of cyclic adenosine monophosphate (cAMP) activated by cytosolic calmodulin, was used as a reporter for protein delivery in living cells. A fusion protein combining MBP, RFP, CyaA, and His20 (MBP-RFP-CyaA-His20) was delivered into plant cells and increased intracellular fluorescence and cAMP production in all cell lines. The present study demonstrates that PHPs are effective carriers of proteins into the intracellular space of various cultured plant cells.

scholarly journals Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from Bradyrhizobium japonicum USDA110

2002 ◽  
Vol 43 (11) ◽  
pp. 1314-1322
Author(s):  
Tsuneo Hakoyama ◽  
Tadashi Yokoyama ◽  
Hiroshi Kouchi ◽  
Ken-ichi Tsuchiya ◽  
Hisatoshi Kaku ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Cultured Cells ◽  
Transcriptional Response ◽  
Nod Factors ◽  
Suspension Cultured Cells

Phytotropins. IV. Effect of Phytotropins on Cultured Plant Cells and Protoplasts

1982 ◽  
Vol 9 (3) ◽  
pp. 297 ◽  
Author(s):  
PJ Larkin ◽  
W Scowcroft ◽  
AE Geissler ◽  
GF Katekar
Keyword(s):  
Petunia Hybrida ◽  
Cultured Cells ◽  
Plant Cells ◽  
Dichlorophenoxyacetic Acid ◽  
Polar Transport ◽  
Suspension Cultured Cells ◽  
Specific Receptors ◽  
Nicotiana Debneyi ◽  
2,4 Dichlorophenoxyacetic Acid

The phytotropins l-(2'-carboxyphenyl)-3-phenylpropane-1,3-dione (CPP) and 5-(2-carboxyphenyl)- 3-phenylpyrazole (CPD) reduced the net efflux of radiolabel from suspension-cultured cells treated with [14C]2,4-dichlorophenoxyacetic acid when present at concentrations comparable to those that inhibit polar transport of auxins in bean petioles. These phytotropins stimulated division of protoplasts of both Nicotiana debneyi and Petunia hybrida at concentrations of exogenous auxins that were otherwise suboptimal for divisions. The results are consistent with the proposal that phytotropins interact with specific receptors to reduce auxin efflux, resulting in increased intracellular auxin concentrations.

Mevalonate kinase activity in Catharanthus roseus plants and suspension cultured cells

Plant Science ◽  
2000 ◽  
Vol 150 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Anna E Schulte ◽  
Eva M Llamas Durán ◽  
Robert van der Heijden ◽  
Robert Verpoorte
Keyword(s):  
Catharanthus Roseus ◽  
Kinase Activity ◽  
Cultured Cells ◽  
Mevalonate Kinase ◽  
Suspension Cultured Cells

A lipochito-oligosaccharide, Nod factor, induces transient calcium influx in soybean suspension-cultured cells

2000 ◽  
Vol 22 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Tadashi Yokoyama ◽  
Naoki Kobayashi ◽  
Hiroshi Kouchi ◽  
Kiwamu Minamisawa ◽  
Hisatoshi Kaku ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Calcium Influx ◽  
Nod Factor ◽  
Suspension Cultured Cells
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