Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform

2006 ◽  
Vol 1 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Bruce S Edwards ◽  
Susan M Young ◽  
Tudor I Oprea ◽  
Cristian G Bologa ◽  
Eric R Prossnitz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Receptor Ligands ◽  
Formylpeptide Receptor

High-Throughput Screening with HyperCyt® Flow Cytometry to Detect Small Molecule Formylpeptide Receptor Ligands

2005 ◽  
Vol 10 (4) ◽  
pp. 374-382 ◽  
Author(s):  
Susan M. Young ◽  
Cristian Bologa ◽  
Eric R. Prossnitz ◽  
Tudor I. Oprea ◽  
Larry A. Sklar ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Receptor Ligands ◽  
Chemical Library ◽  
Assay Sensitivity ◽  
Compound Libraries ◽  
Analysis System ◽  
G Protein Coupled ◽  
Formylpeptide Receptor

High-throughput flow cytometry (HTFC), enabled by faster automated sample processing, represents a promising high- content approach for compound library screening. HyperCyt® is a recently developed automated HTFC analysis system by which cell samples are rapidly aspirated from microplate wells and delivered to the flow cytometer. The formylpeptide receptor (FPR) family of G protein–coupled receptors contributes to the localization and activation of tissue-damaging leukocytes at sites of chronic inflammation. Here, the authors describe development and application of an HTFC screening approach to detect potential anti-inflammatory compounds that block ligand binding to FPR. Using a homogeneous no-wash assay, samples were routinely processed at 1.5 s/well (~2500 cells analyzed/sample), allowing a 96-well plate to be processed in less than 2.5 min. Assay sensitivity and accuracy were validated by detection of a previously documented active compound with relatively low FPR affinity (sulfinpyrazone, inhibition constant [Ki]=14 μM) from among a collection of 880 compounds in the Prestwick Chemical Library. The HyperCyt® system was therefore demonstrated to be a robust, sensitive, and highly quantitative method with which to screen lead compound libraries in a 96-well format.

scholarly journals Duplex high-throughput flow cytometry screen identifies two novel formylpeptide receptor family probes

2009 ◽  
Vol 75A (3) ◽  
pp. 253-263 ◽  
Author(s):  
Susan M. Young ◽  
Cristian M. Bologa ◽  
Dan Fara ◽  
Bj K. Bryant ◽  
Juan Jacob Strouse ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Receptor Family ◽  
Formylpeptide Receptor

Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide Receptor Antagonists

2005 ◽  
Vol 68 (5) ◽  
pp. 1301-1310 ◽  
Author(s):  
Bruce S. Edwards ◽  
Cristian Bologa ◽  
Susan M. Young ◽  
Konstantin V. Balakin ◽  
Eric R. Prossnitz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Virtual Screening ◽  
Small Molecule ◽  
High Throughput ◽  
Receptor Antagonists ◽  
Formylpeptide Receptor

A deep learning-based concept for high throughput image flow cytometry

2021 ◽  
Vol 118 (12) ◽  
pp. 123701
Author(s):  
Julie Martin-Wortham ◽  
Steffen M. Recktenwald ◽  
Marcelle G. M. Lopes ◽  
Lars Kaestner ◽  
Christian Wagner ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Deep Learning ◽  
High Throughput ◽  
Image Flow

scholarly journals High-throughput multiparametric imaging flow cytometry: toward diffraction-limited sub-cellular detection and monitoring of sub-cellular processes

Cell Reports ◽  
2021 ◽  
Vol 34 (10) ◽  
pp. 108824
Author(s):  
Gregor Holzner ◽  
Bogdan Mateescu ◽  
Daniel van Leeuwen ◽  
Gea Cereghetti ◽  
Reinhard Dechant ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Multiparametric Imaging ◽  
Cellular Processes ◽  
Imaging Flow Cytometry

scholarly journals A “No‐Touch” Antibody‐Staining Method of Adherent Cells for High‐Throughput Flow Cytometry in 384‐Well Microplate Format for Cell‐Based Drug Library Screening

2019 ◽  
Vol 97 (8) ◽  
pp. 845-851 ◽  
Author(s):  
Annelisa M. Cornel ◽  
Celina L. Szanto ◽  
Niek P. Til ◽  
Jeroen F. Velzen ◽  
Jaap J. Boelens ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Staining Method ◽  
Adherent Cells ◽  
Library Screening ◽  
Antibody Staining

High-Throughput Raman Flow Cytometry and Beyond

2021 ◽  
Author(s):  
Julia Gala de Pablo ◽  
Matthew Lindley ◽  
Kotaro Hiramatsu ◽  
Keisuke Goda
Keyword(s):  
Flow Cytometry ◽  
High Throughput

scholarly journals Recent Bioinformatics Advances in the Analysis of High Throughput Flow Cytometry Data

2009 ◽  
Vol 2009 ◽  
pp. 1-2
Author(s):  
Raphael Gottardo ◽  
Ryan R. Brinkman ◽  
George Luta ◽  
Matt P. Wand
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Flow Cytometry Data
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