Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images

2006 ◽  
Vol 1 (4) ◽  
pp. 2152-2161 ◽  
Author(s):  
Alfredo Rodriguez ◽  
Douglas B Ehlenberger ◽  
Patrick R Hof ◽  
Susan L Wearne
Keyword(s):  
Shape Analysis ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Dimensional Shape ◽  
Three Dimensional Shape ◽  
Microscopy Images

Automated reconstruction of three-dimensional neuronal morphology from laser scanning microscopy images

Methods ◽  
2003 ◽  
Vol 30 (1) ◽  
pp. 94-105 ◽  
Author(s):  
Alfredo Rodriguez ◽  
Douglas Ehlenberger ◽  
Kevin Kelliher ◽  
Michael Einstein ◽  
Scott C. Henderson ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Neuronal Morphology ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Microscopy Images

Behaviour of nucleolar proteins in nuclei lacking ribosomal genes. A study by confocal laser scanning microscopy

1991 ◽  
Vol 98 (1) ◽  
pp. 99-105
Author(s):  
D. Hernandez-Verdun ◽  
M. Robert-Nicoud ◽  
G. Geraud ◽  
C. Masson
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Specific Marker ◽  
Nucleolar Proteins ◽  
Fibrillar Component

The behaviour of nucleolar proteins in cycling PtK1 cells and in micronuclei with or without NORs was investigated by immunofluorescence using antibodies from autoimmune sera and confocal laser scanning microscopy. These antibodies were shown by electron microscopy to recognize antigens confined to only one of the three basic nucleolar components: fibrillar centres (FC), dense fibrillar component (DFC) and granular component (GC). Serial optical sections allowed us to determine the three-dimensional organization of these components in the nucleolus of cycling cells. Furthermore, clear differences were found in the distribution of the various antigens in micronucleated cells. Three patterns could be observed: (1) the FC antigens were found mainly in the nucleoli, but also in varying amounts in the dots; (2) surprisingly, the DFC antigens were found to accumulate preferentially in the dots; (3) the GC-specific marker stained intensively the nucleoli as well the dots. The results are interpreted with regard to possible mechanisms for targeting nucleolar proteins to the site of nucleolar formation.

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