scholarly journals Mass Spectrometry Imaging, an Emerging Technology in Neuropsychopharmacology

2013 ◽  
Vol 39 (1) ◽  
pp. 34-49 ◽  
Author(s):  
Mohammadreza Shariatgorji ◽  
Per Svenningsson ◽  
Per E Andrén
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Emerging Technology

Mass spectrometry imaging: An emerging technology for the analysis of metabolites in insects

2019 ◽  
Vol 103 (4) ◽  
Author(s):  
Fei‐Ying Yang ◽  
Jun‐Hui Chen ◽  
Qian‐Qian Ruan ◽  
Hafiz S. A. Saqib ◽  
Wei‐Yi He ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Emerging Technology

scholarly journals Current Advances in Mass Spectrometry Imaging for Insect Physiology and Metabolism

2020 ◽  
Author(s):  
Fei-Ying Yang ◽  
Wei-Yi He ◽  
Min-Sheng You
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Molecular Biology ◽  
Mass Spectrometry Imaging ◽  
Emerging Technology ◽  
Spatiotemporal Distribution ◽  
Insect Physiology ◽  
Low Efficiency ◽  
Underlying Mechanisms

Research regarding the distribution of metabolites is a vital aspect of insect molecular biology. However, current approaches (e.g., liquid chromatography-mass spectrometry or immunofluorescence) have cons like requirement of massive tissues, low efficiency, and complicated operating processes. As an emerging technology, mass spectrometry imaging (MSI) can visualize the spatiotemporal distribution of molecules in biological samples without labeling. In this chapter, we retrospect the major types of in situ measurement by MSI, and the application of MSI for investigating insect endogenous and exogenous metabolites and monitoring the dynamic changes of metabolites involved with the interactions between insects and plants. Future studies that combine MSI with other genetic tools can facilitate to better explore the underlying mechanisms concerning insect physiology and metabolism.

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

scholarly journals Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in Gammarus fossarum lipid composition

iScience ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 102115
Author(s):  
Tingting Fu ◽  
Oskar Knittelfelder ◽  
Olivier Geffard ◽  
Yohann Clément ◽  
Eric Testet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lipid Composition ◽  
Mass Spectrometry Imaging ◽  
Shotgun Lipidomics ◽  
Gammarus Fossarum

scholarly journals Diagnosis of Agglomeration and Crystallinity of Active Pharmaceutical Ingredients in Over the Counter Headache Medication by Electrospray Laser Desorption Ionization Mass Spectrometry Imaging

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 610
Author(s):  
Mariann Inga Van Meter ◽  
Salah M. Khan ◽  
Brynne V. Taulbee-Cotton ◽  
Nathan H. Dimmitt ◽  
Nathan D. Hubbard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Laser Desorption ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Active Pharmaceutical Ingredients ◽  
Laser Desorption Ionization ◽  
Over The Counter ◽  
Pharmaceutical Ingredients ◽  
Desorption Ionization

Agglomeration of active pharmaceutical ingredients (API) in tablets can lead to decreased bioavailability in some enabling formulations. In a previous study, we determined that crystalline APIs can be detected as agglomeration in tablets formulated with amorphous acetaminophen tablets. Multiple method advancements are presented to better resolve agglomeration caused by crystallinity in standard tablets. In this study, we also evaluate three “budget” over-the-counter headache medications (subsequently labeled as brands A, B, and C) for agglomeration of the three APIs in the formulation: Acetaminophen, aspirin, and caffeine. Electrospray laser desorption ionization mass spectrometry imaging (ELDI-MSI) was used to diagnose agglomeration in the tablets by creating molecular images and observing the spatial distributions of the APIs. Brand A had virtually no agglomeration or clustering of the active ingredients. Brand B had extensive clustering of aspirin and caffeine, but acetaminophen was observed in near equal abundance across the tablet. Brand C also had extensive clustering of aspirin and caffeine, and minor clustering of acetaminophen. These results show that agglomeration with active ingredients in over-the-counter tablets can be simultaneously detected using ELDI-MS imaging.

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