scholarly journals Reversal of Dopamine Inhibition of Dopaminergic Neurons of the Ventral Tegmental Area is Mediated by Protein Kinase C

2011 ◽  
Vol 37 (2) ◽  
pp. 543-556 ◽  
Author(s):  
Sudarat Nimitvilai ◽  
Devinder S Arora ◽  
Mark S Brodie
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Ventral Tegmental Area ◽  
Dopaminergic Neurons ◽  
Kinase C ◽  
Ventral Tegmental

Serotonin Reduces the Hyperpolarization-Activated Current (Ih) in Ventral Tegmental Area Dopamine Neurons: Involvement of 5-HT2 Receptors and Protein Kinase C

2003 ◽  
Vol 90 (5) ◽  
pp. 3201-3212 ◽  
Author(s):  
Zhaoping Liu ◽  
E. Bradshaw Bunney ◽  
Sarah B. Appel ◽  
Mark S. Brodie
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Ventral Tegmental Area ◽  
Kinase Inhibitor ◽  
Brain Slices ◽  
Dopamine Neurons ◽  
Dependent Manner ◽  
Cationic Current ◽  
Kinase C ◽  
Ventral Tegmental

Dopaminergic neurons of the ventral tegmental area (VTA) have been implicated in the rewarding properties of drugs of abuse and in the etiology of schizophrenia; serotonin modulation of these neurons may play a role in these phenomena. Whole cell patch-in-the-slice recording in rat brain slices was used to investigate modulation of the hyperpolarization-activated cationic current Ih by serotonin in these neurons. Serotonin (50-500 μM) reduced the amplitude of Ih in a concentration-dependent manner; this effect was reversible after prolonged washout of serotonin. This effect was mimicked by the 5-HT2 agonist α-methylserotonin (25 μM) and reversed by the 5-HT2 antagonist ketanserin (25 μM). Serotonin reduced the maximal Ih current and conductance (measured at -130 mV) and caused a negative shift in the voltage dependence of Ih activation. The serotonin-induced reduction in Ih amplitude was antagonized by intracellular administration of the nonspecific protein kinase inhibitor H-7 (75 μM) and the selective protein kinase C inhibitor chelerythrine (25 μM). The protein kinase C activator phorbol 12, 13 diacetate (PDA, 2 μM) reduced Ih amplitude; when PDA and serotonin were applied together, the effect on Ih was less than additive. These data support the conclusion that serotonin reduces Ih in dopaminergic VTA neurons by acting at serotonin 5-HT2 receptors, which activate protein kinase C. This reduction of Ih may be physiologically important, as the selective inhibitor of Ih, ZD7288, significantly increased dopamine inhibition of firing rate of dopaminergic VTA neurons, an effect that we previously demonstrated with serotonin.

Orexin-a activates phospholipase C- and protein kinase C-mediated Ca2+ signaling in dopamine neurons of the ventral tegmental area

Neuroreport ◽  
2001 ◽  
Vol 12 (9) ◽  
pp. 1885-1889 ◽  
Author(s):  
Kazuhide Uramura ◽  
Hisayuki Funahashi ◽  
Shinji Muroya ◽  
Seiji Shioda ◽  
Morikuni Takigawa ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Ventral Tegmental Area ◽  
Phospholipase C ◽  
Dopamine Neurons ◽  
Orexin A ◽  
Kinase C ◽  
Ventral Tegmental
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