Single-molecule chemical reactions on DNA origami

2010 ◽  
Vol 5 (3) ◽  
pp. 200-203 ◽  
Author(s):  
Niels V. Voigt ◽  
Thomas Tørring ◽  
Alexandru Rotaru ◽  
Mikkel F. Jacobsen ◽  
Jens B. Ravnsbæk ◽  
...  
Keyword(s):  
Chemical Reactions ◽  
Single Molecule ◽  
Dna Origami

scholarly journals Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement

ACS Nano ◽  
2021 ◽  
Author(s):  
Rishabh M. Shetty ◽  
Sarah R. Brady ◽  
Paul W. K. Rothemund ◽  
Rizal F. Hariadi ◽  
Ashwin Gopinath
Keyword(s):  
Single Molecule ◽  
Dna Origami

DNA Origami as a DNA Repair Nanosensor at the Single-Molecule Level

2013 ◽  
Vol 52 (30) ◽  
pp. 7747-7750 ◽  
Author(s):  
Maria Tintoré ◽  
Isaac Gállego ◽  
Brendan Manning ◽  
Ramon Eritja ◽  
Carme Fàbrega
Keyword(s):  
Dna Repair ◽  
Single Molecule ◽  
Dna Origami ◽  
Single Molecule Level

DNA Origami Nanoantennas with over 5000-fold Fluorescence Enhancement and Single-Molecule Detection at 25 μM

Nano Letters ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 8354-8359 ◽  
Author(s):  
Anastasiya Puchkova ◽  
Carolin Vietz ◽  
Enrico Pibiri ◽  
Bettina Wünsch ◽  
María Sanz Paz ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Dna Origami ◽  
Single Molecule Detection

scholarly journals Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites

2019 ◽  
Author(s):  
Helen L. Miller ◽  
Sonia Contera ◽  
Adam J.M. Wollman ◽  
Adam Hirst ◽  
Katherine E. Dunn ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Single Molecule ◽  
Targeted Drug Delivery ◽  
Binding Sites ◽  
Dna Origami ◽  
Target Cells ◽  
Dna Nanostructures ◽  
Synthetic Dna ◽  
Drug Molecules ◽  
Targeted Drug

AbstractIntercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery. This is due to the excellent biocompatibility of synthetic DNA nanostructures, and high potential for flexible programmability including facile drug release into or near to target cells. Such favourable properties may enable high initial loading and efficient release for a predictable number of drug molecules per nanostructure carrier, important for efficient delivery of safe and effective drug doses to minimise non-specific release away from target cells. However, basic questions remain as to how intercalation-mediated loading depends on the DNA carrier structure. Here we use the interaction of dyes YOYO-1 and acridine orange with a tightly-packed 2D DNA origami tile as a simple model system to investigate intercalation-mediated loading. We employed multiple biophysical techniques including single-molecule fluorescence microscopy, atomic force microscopy, gel electrophoresis and controllable damage using low temperature plasma on synthetic DNA origami samples. Our results indicate that not all potential DNA binding sites are accessible for dye intercalation, which has implications for future DNA nanostructures designed for targeted drug delivery.

Single molecule microscopy methods for the study of DNA origami structures

2010 ◽  
Vol 74 (7) ◽  
pp. 688-698 ◽  
Author(s):  
Victoria Birkedal ◽  
Mingdong Dong ◽  
Monika M. Golas ◽  
Bjoern Sander ◽  
Ebbe Sloth Andersen ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Origami ◽  
Single Molecule Microscopy

scholarly journals Arranging Small Molecules with Subnanometer Precision on DNA Origami Substrates for the Single‐Molecule Investigation of Protein–Ligand Interactions

2020 ◽  
Vol 1 (1) ◽  
pp. 2000038
Author(s):  
Jingyuan Huang ◽  
Antonio Suma ◽  
Meiying Cui ◽  
Guido Grundmeier ◽  
Vincenzo Carnevale ◽  
...  
Keyword(s):  
Small Molecules ◽  
Single Molecule ◽  
Dna Origami ◽  
Protein Ligand Interactions ◽  
Ligand Interactions
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