Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli

Tobias Cornvik; Sue-Li Dahlroth; Audur Magnusdottir; Maria Dolores Herman; Rosemarie Knaust; Monica Ekberg; Pär Nordlund

doi:10.1038/nmeth767

Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli

Nature Methods ◽

10.1038/nmeth767 ◽

2005 ◽

Vol 2 (7) ◽

pp. 507-509 ◽

Cited By ~ 54

Author(s):

Tobias Cornvik ◽

Sue-Li Dahlroth ◽

Audur Magnusdottir ◽

Maria Dolores Herman ◽

Rosemarie Knaust ◽

...

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Soluble Protein ◽

Screening Method ◽

Expression In Escherichia Coli

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Framework for the Rapid Optimization of Soluble Protein Expression in Escherichia coli Combining Microscale Experiments and Statistical Experimental Design

Biotechnology Progress ◽

10.1021/bp070059a ◽

2007 ◽

Vol 23 (4) ◽

pp. 785-793 ◽

Cited By ~ 11

Author(s):

R.S. Islam ◽

D. Tisi ◽

M.S. Levy ◽

G.J. Lye

Keyword(s):

Escherichia Coli ◽

Experimental Design ◽

Protein Expression ◽

Soluble Protein ◽

Statistical Experimental Design ◽

Expression In Escherichia Coli

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The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology

Applied Microbiology and Biotechnology ◽

10.1007/s00253-012-4559-1 ◽

2012 ◽

Vol 97 (15) ◽

pp. 6779-6791 ◽

Cited By ~ 45

Author(s):

Sofia J. Costa ◽

André Almeida ◽

António Castro ◽

Lucília Domingues ◽

Hüseyin Besir

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Soluble Protein ◽

Gene Fusion ◽

The Novel ◽

Fusion Technology ◽

Expression In Escherichia Coli

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Characterization of a cellulose binding domain from Clostridium cellulovorans endoglucanase-xylanase D and its use as a fusion partner for soluble protein expression in Escherichia coli

Journal of Biotechnology ◽

10.1016/j.jbiotec.2008.05.004 ◽

2008 ◽

Vol 135 (4) ◽

pp. 319-325 ◽

Cited By ~ 13

Author(s):

Yin Xu ◽

Frances C. Foong

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Soluble Protein ◽

Binding Domain ◽

Fusion Partner ◽

Cellulose Binding Domain ◽

Clostridium Cellulovorans ◽

Expression In Escherichia Coli ◽

Cellulose Binding

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A comparison of statistical approaches used for the optimization of soluble protein expression in Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2015.12.014 ◽

2016 ◽

Vol 120 ◽

pp. 126-137 ◽

Cited By ~ 10

Author(s):

Christos Papaneophytou ◽

George Kontopidis

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Soluble Protein ◽

Expression In Escherichia Coli ◽

Statistical Approaches

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Screening of genetic parameters for soluble protein expression in Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2010.11.016 ◽

2011 ◽

Vol 77 (1) ◽

pp. 104-111 ◽

Cited By ~ 10

Author(s):

Erik Vernet ◽

Alexander Kotzsch ◽

Bjørn Voldborg ◽

Michael Sundström

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Soluble Protein ◽

Genetic Parameters ◽

Expression In Escherichia Coli

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Strategies for optimizing heterologous protein expression in Escherichia coli

Trends in Biotechnology ◽

10.1016/s0167-7799(97)01155-4 ◽

1998 ◽

Vol 16 (2) ◽

pp. 54-60 ◽

Cited By ~ 295

Author(s):

Gerhard Hannig ◽

Savvas C Makrides

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Heterologous Protein ◽

Heterologous Protein Expression ◽

Expression In Escherichia Coli

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Recombinant protein expression in Escherichia coli: advances and challenges

Frontiers in Microbiology ◽

10.3389/fmicb.2014.00172 ◽

2014 ◽

Vol 5 ◽

Cited By ~ 622

Author(s):

GermÃ¡n L. Rosano ◽

Eduardo A. Ceccarelli

Keyword(s):

Escherichia Coli ◽

Recombinant Protein ◽

Protein Expression ◽

Recombinant Protein Expression ◽

Expression In Escherichia Coli

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Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Microbial Cell Factories ◽

10.1186/1475-2859-8-68 ◽

2009 ◽

Vol 8 (1) ◽

pp. 68 ◽

Cited By ~ 63

Author(s):

Frank Kensy ◽

Christoph Engelbrecht ◽

Jochen Büchs

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Online Monitoring ◽

Hansenula Polymorpha ◽

Scale Up ◽

Microtiter Plate ◽

Monitoring Techniques ◽

Expression In Escherichia Coli

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Protein expression in Escherichia coli S17-1 biofilms: impact of indole

Antonie van Leeuwenhoek ◽

10.1007/s10482-006-9097-3 ◽

2006 ◽

Vol 91 (1) ◽

pp. 71-85 ◽

Cited By ~ 22

Author(s):

Anthony Collet ◽

Sébastien Vilain ◽

Pascal Cosette ◽

Guy Alain Junter ◽

Thierry Jouenne ◽

...

Keyword(s):

Escherichia Coli ◽

Protein Expression ◽

Expression In Escherichia Coli

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Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli

Protein Science ◽

10.1002/pro.669 ◽

2011 ◽

Vol 20 (8) ◽

pp. 1411-1420 ◽

Cited By ~ 42

Author(s):

Brent L. Nannenga ◽

François Baneyx

Keyword(s):

Escherichia Coli ◽

Membrane Protein ◽

Protein Expression ◽

Expression In Escherichia Coli ◽

Membrane Protein Expression

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