Quantitative analysis of gene expression in living adult neural stem cells by gene trapping

2005 ◽  
Vol 2 (5) ◽  
pp. 363-370 ◽  
Author(s):  
John R Scheel ◽  
Jasodhara Ray ◽  
Fred H Gage ◽  
Carrolee Barlow
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Quantitative Analysis ◽  
Neural Stem Cells ◽  
Adult Neural Stem Cells ◽  
Gene Trapping ◽  
Living Adult

Catalog of gene expression in adult neural stem cells and their in vivo microenvironment

2006 ◽  
Vol 312 (10) ◽  
pp. 1798-1812 ◽  
Author(s):  
Cecilia Williams ◽  
Valtteri Wirta ◽  
Konstantinos Meletis ◽  
Lilian Wikström ◽  
Leif Carlsson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Adult Neural Stem Cells

scholarly journals Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
You Ning ◽  
Jian-Hua Huang ◽  
Shi-Jin Xia ◽  
Qin Bian ◽  
Yang Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Dna Microarray ◽  
Specific Gene ◽  
Specific Marker ◽  
Dependent Manner ◽  
Adult Neural Stem Cells ◽  
Medicine Research ◽  
Novel Treatments

Adult neural stem cells (NSCs) persist throughout life to replace mature cells that are lost during turnover, disease, or injury. The investigation of NSC creates novel treatments for central nervous system (CNS) injuries and neurodegenerative disorders. The plasticity and reparative potential of NSC are regulated by different factors, which are critical for neurological regenerative medicine research. We investigated the effects of Psoralen, which is the mature fruit ofPsoralea corylifolia L., on NSC behaviors and the underlying mechanisms. The self-renewal and proliferation of NSC were examined. We detected neuron- and/or astrocyte-specific markers using immunofluorescence and Western blotting, which could evaluate NSC differentiation. Psoralen treatment significantly inhibited neurosphere formation in a dose-dependent manner. Psoralen treatment increased the expression of the astrocyte-specific marker but decreased neuron-specific marker expression. These results suggested that Psoralen was a differentiation inducer in astrocyte. Differential gene expression following Psoralen treatment was screened using DNA microarray and confirmed by quantitative real-time PCR. Our microarray study demonstrated that Psoralen could effectively regulate the specific gene expression profile of NSC. The genes involved in the classification of cellular differentiation, proliferation, and metabolism, the transcription factors belonging to Ets family, and the hedgehog pathway may be closely related to the regulation.

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