scholarly journals Optimized library preparation method for next-generation sequencing

2009 ◽  
Vol 6 (10) ◽  
pp. i-ii ◽  
Author(s):  
Fraz Syed ◽  
Haiying Grunenwald ◽  
Nicholas Caruccio
Keyword(s):  
Next Generation Sequencing ◽  
Preparation Method ◽  
Library Preparation ◽  
Next Generation ◽  
Generation Sequencing ◽  
Library Preparation Method

scholarly journals Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Wells W. Wu ◽  
Je-Nie Phue ◽  
Chun-Ting Lee ◽  
Changyi Lin ◽  
Lai Xu ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Throughput ◽  
Library Preparation ◽  
Next Generation ◽  
Generation Sequencing

Transcriptome-wide m6A detection with DART-Seq

2019 ◽  
Author(s):  
Kate D. Meyer
Keyword(s):  
Gene Expression Regulation ◽  
Preparation Method ◽  
Current Knowledge ◽  
Cross Reactivity ◽  
High Variability ◽  
Library Preparation ◽  
Rna Seq ◽  
Generation Sequencing ◽  
Library Preparation Method

Abstract m6A is the most abundant internal mRNA modification and plays diverse roles in gene expression regulation. Much of our current knowledge about m6A has been driven by recent advances in the ability to detect this mark transcriptome-wide. Antibody-based approaches have been the method of choice for global m6A mapping studies. These methods rely on m6A antibodies to immunoprecipitate methylated RNAs, followed by next-generation sequencing to identify m6A-containing transcripts1,2. While these methods enabled the first identification of m6A sites transcriptome-wide and have dramatically improved our ability to study m6A, they suffer from several limitations. These include requirements for high amounts of input RNA, costly and time-consuming library preparation, high variability across studies, and m6A antibody cross-reactivity with other modifications. Here, we describe DART-Seq (deamination adjacent to RNA modification targets), an antibody-free method for global m6A detection. In DART-Seq, the C to U deaminating enzyme, APOBEC1, is fused to the m6A-binding YTH domain. This fusion protein is then introduced to cellular RNA either through overexpression in cells or with in vitro assays, and subsequent deamination of m6A-adjacent cytidines is then detected by RNA sequencing to identify m6A sites. DART-Seq can successfully map m6A sites throughout the transcriptome using as little as 10 nanograms of total cellular RNA, and it is compatible with any standard RNA-seq library preparation method.

scholarly journals Rapid, high-throughput library preparation for next-generation sequencing

2010 ◽  
Vol 7 (8) ◽  
pp. iii-iv ◽  
Author(s):  
Haiying Grunenwald ◽  
Brad Baas ◽  
Nicholas Caruccio ◽  
Fraz Syed
Keyword(s):  
Next Generation Sequencing ◽  
High Throughput ◽  
Library Preparation ◽  
Next Generation ◽  
Generation Sequencing

201-P Use of the Fluidigm® access Array™ system provides simplified amplicon library preparation in next generation sequencing for high throughput HLA genotyping

2011 ◽  
Vol 72 ◽  
pp. S142 ◽  
Author(s):  
Priscilla V. Moonsamy ◽  
Persia L. Bonella ◽  
Timothy C. Williams ◽  
Cherie L. Holcomb ◽  
Gregory S. Turenchalk ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Throughput ◽  
Library Preparation ◽  
Next Generation ◽  
Amplicon Library ◽  
Hla Genotyping ◽  
Generation Sequencing
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