Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells

2015 ◽  
Vol 12 (9) ◽  
pp. 885-892 ◽  
Author(s):  
Daniel Paull ◽  
Ana Sevilla ◽  
Hongyan Zhou ◽  
Aana Kim Hahn ◽  
Hesed Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells

2017 ◽  
Vol 89 (4) ◽  
pp. 2440-2448 ◽  
Author(s):  
Anna Baud ◽  
Frank Wessely ◽  
Francesca Mazzacuva ◽  
James McCormick ◽  
Stephane Camuzeaux ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals High-throughput propagation of human prostate tissue from induced-pluripotent stem cells

2019 ◽  
Author(s):  
AC Hepburn ◽  
EL Curry ◽  
M Moad ◽  
RE Steele ◽  
OE Franco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Neuroendocrine Cells ◽  
Prostate Tissue ◽  
Urogenital Sinus ◽  
Human Prostate ◽  
Human Prostate Tissue ◽  
Induced Pluripotent

AbstractPrimary culture of human prostate organoids is slow, inefficient and laborious. To overcome this, we demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells, for the first time, enable generation of human prostate tissue in vivo and in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. This approach overcomes major limitations in primary cultures of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. These models provide new opportunities to study prostate development, homeostasis and disease.

scholarly journals Single-cell phenotyping of human induced pluripotent stem cells by high-throughput imaging

2015 ◽  
Author(s):  
Hans Christian Volz ◽  
Florian Heigwer ◽  
Tatjana Wuest ◽  
Marta Galach ◽  
Jochen Utikal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Single Cell ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Single Cells ◽  
Phenotypic Variability ◽  
Screening Experiments ◽  
Induced Pluripotent ◽  
High Throughput Imaging

Single-cell phenotyping promises to yield insights into biological responses in heterogeneous cell populations. We developed a method based on single-cell analysis to phenotype human induced pluripotent stem cells (hIPSC) by high-throughput imaging. Our method uses markers for morphology and pluripotency as well as social features to characterize perturbations using a meta-phenotype based on mapping single cells to distinct phenotypic classes. Analysis of perturbations on a single cell level enhances the applicability of human induced pluripotent stem cells (hIPSC) for screening experiments taking the inherently increased phenotypic variability of these cells into account. We adapted miniaturized culture conditions to allow for the utilization of hIPSC in RNA interference (RNAi) high-throughput screens and single cell phenotyping by image analysis. We identified key regulators of pluripotency in hIPSC masked in a population-averaged analysis and we confirmed several candidate genes (SMG1, TAF1) and assessed their effect on pluripotency.

scholarly journals High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells

2017 ◽  
Vol 8 (4) ◽  
pp. 1101-1111 ◽  
Author(s):  
Matteo D'Antonio ◽  
Grace Woodruff ◽  
Jason L. Nathanson ◽  
Agnieszka D'Antonio-Chronowska ◽  
Angelo Arias ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Cost Effective ◽  
Induced Pluripotent

scholarly journals A High-Throughput Method as a Diagnostic Tool for HIV Detection in Patient-Specific Induced Pluripotent Stem Cells Generated by Different Reprogramming Methods

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniela Hübscher ◽  
Sabine Rebs ◽  
Luis Haupt ◽  
Thomas Borchert ◽  
Celina Isabell Guessoum ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Mononuclear Cells ◽  
Sendai Virus ◽  
Patient Specific ◽  
Pathogenic Viruses ◽  
Induced Pluripotent ◽  
Hiv 1

Induced pluripotent stem cells (iPSCs) provide a unique opportunity for generation of patient-specific cells for use in translational purposes. We aimed to compare iPSCs generated by different reprogramming methods regarding their reprogramming efficiency, pluripotency capacity, and the possibility to use high-throughput PCR-based methods for detection of human pathogenic viruses. iPSCs from skin fibroblasts (FB), peripheral blood mononuclear cells (PBMCs), or mesenchymal stem cells (MSCs) were generated by using three different reprogramming systems including chromosomal integrating and nonintegrating methods. Reprogramming efficiencies were in accordance with the literature, indicating that the parental cell type and the reprogramming method play a major role for the reprogramming efficiencies (FB: STEMCCA: 1.30±0.18, Sendai virus: 1.37±0.01, and episomal plasmids: 0.04±0.02; PBMCs: Sendai virus: 0.002±0.001, episomal plasmids: 0) but result in the same characteristics of pluripotency. We found the highest reprogramming efficiencies for MSC with 3.32±1.2 by using episomal plasmids. Since GMP standard working procedures and screening units need virus contamination-free cell lines, we studied HIV-1 contamination in the generated iPSCs. We used the high-throughput cobas® 6800/8800 system, which is normally used for detection of HIV-1 in plasma of patients, and found that footprint-free reprogramming methods as episomal plasmids and Sendai virus are useful for the described virus detection method. This fast, cost-effective, robust, and reliable assay demonstrates the feasibility to use high-throughput PCR-based methods for detection of human pathogenic viruses in ps-iPSC lines that were generated with nongenome integrating reprogramming methods.

Use of CRISPR/Cas ribonucleoproteins for high throughput gene editing of induced pluripotent stem cells

Methods ◽  
2021 ◽  
Author(s):  
Qi Wang ◽  
Sueanne Chear ◽  
Kristof Wing ◽  
David Stellon ◽  
Minh Thuan Nguyen Tran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Gene Editing ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document