A general design strategy for protein-responsive riboswitches in mammalian cells

2014 ◽  
Vol 11 (11) ◽  
pp. 1154-1160 ◽  
Author(s):  
Simon Ausländer ◽  
Pascal Stücheli ◽  
Charlotte Rehm ◽  
David Ausländer ◽  
Jörg S Hartig ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Design Strategy ◽  
General Design

scholarly journals Strategie wzornicze a praktyki projektowe w projektowaniu opakowań

2021 ◽  
Vol 13 ◽  
pp. 157-171
Author(s):  
Mariusz Wszołek
Keyword(s):  
Business Strategy ◽  
Design Strategy ◽  
Package Design ◽  
Packaging Design ◽  
General Design ◽  
Design Practices

This article is about general approaches to designing packages in addition to design strategy, communi-cation strategy and business strategy. Combining different design practices is not an endless repository in this article according to package design — it is just a first attempt at describing general design prac-tices. This article is more of an introduction to strategic packaging design. 

A general design strategy for thermoelectric interface materials in n-type Mg3Sb1.5Bi0.5 single leg used in TEGs

2021 ◽  
pp. 117616
Author(s):  
Xinzhi Wu ◽  
Zhijia Han ◽  
Yongbin Zhu ◽  
Biao Deng ◽  
Kang Zhu ◽  
...  
Keyword(s):  
Design Strategy ◽  
General Design ◽  
Interface Materials

Designing indirect–direct bandgap transitions in double perovskites

2017 ◽  
Vol 4 (4) ◽  
pp. 688-693 ◽  
Author(s):  
T. Thao Tran ◽  
Jessica R. Panella ◽  
Juan R. Chamorro ◽  
Jennifer R. Morey ◽  
Tyrel M. McQueen
Keyword(s):  
Conduction Band ◽  
Design Strategy ◽  
Conduction Band Minimum ◽  
Double Perovskites ◽  
General Design ◽  
Direct Bandgap ◽  
Orbital Character

A general design strategy is presented for tuning the convergence of direct and indirect bandgaps based on chemical adjustment of the s- and p-orbital character of the conduction band minimum.

General Design Strategy for Aromatic Ketone-Based Single-Component Dual-Emissive Materials

2014 ◽  
Vol 6 (4) ◽  
pp. 2279-2284 ◽  
Author(s):  
Xuepeng Zhang ◽  
Tongqing Xie ◽  
Minxin Cui ◽  
Li Yang ◽  
Xingxing Sun ◽  
...  
Keyword(s):  
Design Strategy ◽  
Single Component ◽  
Aromatic Ketone ◽  
General Design

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

Use of Uranium-Labeled Antibodies for Electron Microscopic Localization of Various Antigens in Mammalian Cells

1967 ◽  
Vol 25 ◽  
pp. 136-137
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger
Keyword(s):  
Endoplasmic Reticulum ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Specific Antiserum ◽  
Electron Microscopic ◽  
E Coli ◽  
Cytoplasmic Membranes ◽  
Viral Progeny ◽  
Ultrathin Sections ◽  
Electron Microscopic Localization

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

1972 ◽  
Vol 30 ◽  
pp. 350-351
Author(s):  
K. Shankar Narayan ◽  
Kailash C. Gupta ◽  
Tohru Okigaki
Keyword(s):  
Ultraviolet Light ◽  
Mammalian Cells ◽  
Biological Effects ◽  
Survival Rates ◽  
Chinese Hamster ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Ultrastructural Changes ◽  
Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

Sign in / Sign up

Export Citation Format

Share Document