scholarly journals Multicolor two-photon light-sheet microscopy

2014 ◽  
Vol 11 (6) ◽  
pp. 600-601 ◽  
Author(s):  
Pierre Mahou ◽  
Julien Vermot ◽  
Emmanuel Beaurepaire ◽  
Willy Supatto
Keyword(s):  
Light Sheet ◽  
Two Photon ◽  
Light Sheet Microscopy

scholarly journals Characterization of Scattering Effects in Phantom Samples using Single and Two-Photon Excitation Light Sheet Microscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 195a-196a
Author(s):  
Zeno Lavagnino ◽  
Francesca Cella Zanacchi ◽  
Emiliano Ronzitti ◽  
Ivan Coto Hernandez ◽  
Alberto Diaspro
Keyword(s):  
Light Sheet ◽  
Excitation Light ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Scattering Effects ◽  
Two Photon Excitation

scholarly journals Wide field intravital imaging by two-photon-excitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser

2014 ◽  
Vol 5 (10) ◽  
pp. 3311 ◽  
Author(s):  
Atsushi Maruyama ◽  
Yusuke Oshima ◽  
Hiroko Kajiura-Kobayashi ◽  
Shigenori Nonaka ◽  
Takeshi Imamura ◽  
...  
Keyword(s):  
Pulse Energy ◽  
Light Sheet ◽  
Intravital Imaging ◽  
Wide Field ◽  
High Pulse Energy ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
High Pulse ◽  
Energy Laser

scholarly journals Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation

2013 ◽  
Vol 21 (11) ◽  
pp. 13824 ◽  
Author(s):  
Florian O. Fahrbach ◽  
Vasily Gurchenkov ◽  
Kevin Alessandri ◽  
Pierre Nassoy ◽  
Alexander Rohrbach
Keyword(s):  
Light Sheet ◽  
Bessel Beams ◽  
Fluorescence Excitation ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Two-photon three-axis digital scanned light-sheet microscopy (2P3A-DSLM)

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Weijian Zong ◽  
Xuanyang Chen ◽  
Jia Zhao ◽  
Yunfeng Zhang ◽  
Ming Fan ◽  
...  
Keyword(s):  
Light Sheet ◽  
Two Photon ◽  
Light Sheet Microscopy

scholarly journals Multiview Deconvolution Two-photon Laser Scanning Microscopy

2020 ◽  
Author(s):  
Dimitrios Kapsokalyvas ◽  
Rodrigo Rosas ◽  
Rob Janssen ◽  
Jo Vanoevelen ◽  
Martin Strauch ◽  
...  
Keyword(s):  
Second Harmonic ◽  
Light Sheet ◽  
Laser Scanning Microscopy ◽  
Three Dimensions ◽  
Scanning Microscopy ◽  
Fluorescence Signal ◽  
Microscopy Imaging ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
3D Image Reconstruction

Abstract Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, two-photon and light sheet microscopy. All these techniques suffer from anisotropic resolution and limited penetration depth. In the past, Multiview microscopy - imaging the sample from different angles followed by 3D image reconstruction - was developed to address this issue for light sheet microscopy based on fluorescence signal. In this study we applied this methodology to accomplish Multiview imaging with two-photon microscopy based on fluorescence and additionally second harmonic signal from myosin and collagen. It was shown that isotropic resolution was achieved, the entirety of the sample was visualized, and interference artifacts were suppressed allowing clear visualization of collagen fibrils and myofibrils. This method can be applied to any scanning microscopy technique without microscope modifications. It can be used for imaging tissue and whole mount small organisms such as heart tissue, and zebrafish larva in 3D, label-free or stained, with at least 3-fold axial resolution improvement which can be significant for the accurate quantification of small 3D structures.

scholarly journals Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 337a
Author(s):  
Thai V. Truong ◽  
Daniel B. Holland ◽  
Vikas Trivedi ◽  
Scott E. Fraser
Keyword(s):  
Light Sheet ◽  
Two Photon ◽  
Light Sheet Microscopy

scholarly journals Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sota Takanezawa ◽  
Takashi Saitou ◽  
Takeshi Imamura
Keyword(s):  
High Speed ◽  
Bessel Beam ◽  
Light Sheet ◽  
Time Lapse ◽  
Whole Body ◽  
Wide Field ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Two Photon Excitation

AbstractTwo-photon excitation can lower phototoxicity and improve penetration depth, but its narrow excitation range restricts its applications in light-sheet microscopy. Here, we propose simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams. This unit can stretch the beam full width at half maximum of 600–1000 μm with less than a 4-μm waist when using a 10× illumination lens. A two-photon excitation digital scanned light-sheet microscope possessing this range of field of view and ~2–3-μm axial resolution is constructed and used to analyze the cellular dynamics over the whole body of medaka fish. We demonstrate long-term time-lapse observations over several days and high-speed recording with ~3 mm3 volume per 4 s of the embryos. Our system is minimal and suppresses laser power loss, which can broaden applications of two-photon excitation in light-sheet microscopy.

scholarly journals Live 4D Imaging of the Embryonic Vertebrate Heart with Two-Photon Light Sheet Microscopy and Simultaneous Optical Phase Stamping

2014 ◽  
Vol 106 (2) ◽  
pp. 435a-436a ◽  
Author(s):  
Thai V. Truong ◽  
Vikas Trivedi ◽  
Le Trinh ◽  
Daniel Holland ◽  
Francesco Cutrale ◽  
...  
Keyword(s):  
Light Sheet ◽  
Optical Phase ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
4D Imaging

scholarly journals A versatile, multi-laser twin-microscope system for light-sheet imaging

2019 ◽  
Author(s):  
Kevin Keomanee-Dizon ◽  
Scott E. Fraser ◽  
Thai V. Truong
Keyword(s):  
Near Infrared ◽  
Continuous Wave ◽  
Light Sheet ◽  
Biological Investigation ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Deep Imaging ◽  
Visible Lasers ◽  
Flexible Imaging

Light-sheet microscopy offers faster imaging and reduced phototoxicity in comparison to conventional point-scanning microscopy, making it a preferred technique for imaging biological dynamics for durations of hours or days. Such extended imaging sessions pose a challenge, as it reduces the number of specimens that can be imaged in a given day. Here we present an instrument, the flex-SPIM, that combines two independently controlled light-sheet microscope-twins, built so that they can share an ultrafast near-infrared laser and a bank of continuous-wave visible lasers, increasing throughput and decreasing cost. To permit a wide variety of specimens to be imaged, each microscope-twin provides flexible imaging parameters, including (i) operation in one-photon and/or two-photon excitation modes, (ii) delivery of one to three light-sheets via a trio of orthogonal excitation arms, (iii) sub-micron to micron imaging resolution, (iv) multicolor compatibility, and (v) upright and/or inverted detection geometry. We offer a detailed description of the flex-SPIM design to aid instrument builders who wish to construct and use similar systems. We demonstrate the instrument’s versatility for biological investigation by performing fast imaging of the beating heart in an intact zebrafish embryo, deep imaging of thick patient-derived tumor organoids, and gentle whole-brain imaging of neural activity in behaving larval zebrafish.

scholarly journals Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy

2020 ◽  
Vol 11 (8) ◽  
pp. 4651
Author(s):  
Giuseppe de Vito ◽  
Pietro Ricci ◽  
Lapo Turrini ◽  
Vladislav Gavryusev ◽  
Caroline Müllenbroich ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Light Sheet ◽  
Light Polarization ◽  
Excitation Light ◽  
Two Photon ◽  
Light Sheet Microscopy
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