scholarly journals Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

2014 ◽  
Vol 11 (4) ◽  
pp. 410-412 ◽  
Author(s):  
Lu Wei ◽  
Fanghao Hu ◽  
Yihui Shen ◽  
Zhixing Chen ◽  
Yong Yu ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Live Cell ◽  
Small Biomolecules ◽  
Stimulated Raman

scholarly journals Stimulated Raman scattering of polymer nanoparticles for multiplexed live-cell imaging

2017 ◽  
Vol 53 (46) ◽  
pp. 6187-6190 ◽  
Author(s):  
Fanghao Hu ◽  
Spencer D. Brucks ◽  
Tristan H. Lambert ◽  
Luis M. Campos ◽  
Wei Min
Keyword(s):  
Raman Scattering ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Live Cell ◽  
Polymer Nanoparticles ◽  
Biocompatible Polymer ◽  
Photo Stability ◽  
Stimulated Raman

Biocompatible polymer nanoparticles with vibrational labels incorporated were readily prepared for live-cell multiplexed stimulated Raman scattering imaging with excellent photo-stability.

scholarly journals A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4844-4848 ◽  
Author(s):  
Chen Zeng ◽  
Fanghao Hu ◽  
Rong Long ◽  
Wei Min
Keyword(s):  
Hydrogen Sulfide ◽  
Raman Scattering ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Living Cells ◽  
Live Cell ◽  
Raman Probe ◽  
Stimulated Raman

We develop a ratiometric Raman probe for visualizing hydrogen sulfide in living cells as the first alkyne-based sensor for SRS microscopy.

scholarly journals Label-Free Live-Cell Imaging of Nucleic Acids Using Stimulated Raman Scattering Microscopy

ChemPhysChem ◽  
2012 ◽  
Vol 13 (4) ◽  
pp. 1054-1059 ◽  
Author(s):  
Xu Zhang ◽  
Maarten B. J. Roeffaers ◽  
Srinjan Basu ◽  
Joseph R. Daniele ◽  
Dan Fu ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Nucleic Acids ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Live Cell ◽  
Label Free ◽  
Stimulated Raman

scholarly journals Live-Cell Quantitative Imaging of Proteome Degradation by Stimulated Raman Scattering

2014 ◽  
Vol 53 (22) ◽  
pp. 5596-5599 ◽  
Author(s):  
Yihui Shen ◽  
Fang Xu ◽  
Lu Wei ◽  
Fanghao Hu ◽  
Wei Min
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Live Cell ◽  
Stimulated Raman

scholarly journals Live-Cell Imaging and Quantification of PolyQ Aggregates by Stimulated Raman Scattering of Selective Deuterium Labeling

2019 ◽  
Author(s):  
Kun Miao ◽  
Lu Wei
Keyword(s):  
Raman Scattering ◽  
Live Cell Imaging ◽  
Neurodegenerative Disorder ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Deuterium Labeling ◽  
Multicellular Organisms ◽  
Subcellular Resolution ◽  
Stimulated Raman

AbstractHuntington’s disease, a major neurodegenerative disorder, involves deposition of aggregation-prone proteins with long polyglutamine (polyQ) expansions. The ability to non-perturbatively visualize the formation of aggregates could offer new molecular insight for their pathologic roles. Here, we propose stimulated Raman scattering imaging of deuterium-labeled glutamine to investigate native polyQ aggregates in live cells with subcellular resolution. Through the enrichment of deuterated glutamine in the polyQ sequence of mutant Huntingtin (mHtt) proteins, we first achieved sensitive and specific SRS imaging of carbon-deuterium bonds (C-D) from aggregates without GFP labeling. These aggregates become 1.8-fold denser compared to those with GFP. Second, we performed ratiometric quantification, which revealed a dependence of protein compositions on aggregation sizes. Moreover, we calculated the absolute concentrations for sequestered mHtt and non-mHtt proteins within the same aggregates. Our method may readily reveal new features of polyQ aggregates and could be suited for in vivo investigations on multicellular organisms.

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