scholarly journals A coincidence reporter-gene system for high-throughput screening

2012 ◽  
Vol 9 (10) ◽  
pp. 937-937 ◽  
Author(s):  
Ken C-C Cheng ◽  
James Inglese
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Gene System ◽  
Reporter Gene System

629 A high-throughput cell-based reporter gene system for measuring CYP1A1 induction by xenobiotics in drinking water

2003 ◽  
Vol 144 ◽  
pp. s169
Author(s):  
E. Chiesara ◽  
S. Frigerio ◽  
R. Fumagalli ◽  
L. Marabini ◽  
S. Radice ◽  
...  
Keyword(s):  
Drinking Water ◽  
Reporter Gene ◽  
High Throughput ◽  
Gene System ◽  
Cyp1a1 Induction ◽  
Reporter Gene System

A high-throughput cell-based reporter gene system for measurement of CYP1A1 induction

2002 ◽  
Vol 47 (3) ◽  
pp. 143-151 ◽  
Author(s):  
Xiaoming Cui ◽  
Jairam Palamanda ◽  
Laura Norton ◽  
Ann Thomas ◽  
Yau Yi Lau ◽  
...  
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
Gene System ◽  
Cyp1a1 Induction ◽  
Reporter Gene System

Application of P450RGS reporter gene system as a bioindicator of sediment PAH contamination in the vicinity of Incheon Harbor, Korea

Biomarkers ◽  
1997 ◽  
Vol 2 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Gi Beum Kim ◽  
Jack W. Anderson ◽  
Kristen Bothner ◽  
Jong Hyeon Lee ◽  
Chul Hwan Koh ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Gene System ◽  
Reporter Gene System

Basic Evaluation of FES-hERL PET Tracer-Reporter Gene System for In Vivo Monitoring of Adenoviral-Mediated Gene Therapy

2008 ◽  
Vol 10 (5) ◽  
pp. 245-252 ◽  
Author(s):  
Talakad Goolaiah Lohith ◽  
Takako Furukawa ◽  
Tetsuya Mori ◽  
Masato Kobayashi ◽  
Yasuhisa Fujibayashi
Keyword(s):  
Gene Therapy ◽  
Reporter Gene ◽  
In Vivo Monitoring ◽  
Gene System ◽  
Pet Tracer ◽  
Reporter Gene System ◽  
Basic Evaluation

scholarly journals Use of Leishmania donovani Field Isolates Expressing the Luciferase Reporter Gene in In Vitro Drug Screening

2005 ◽  
Vol 49 (9) ◽  
pp. 3776-3783 ◽  
Author(s):  
Ashutosh ◽  
Suman Gupta ◽  
Ramesh ◽  
Shyam Sundar ◽  
Neena Goyal
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Leishmania Donovani ◽  
Luciferase Activity ◽  
Firefly Luciferase ◽  
Luciferase Reporter ◽  
Luciferase Reporter Gene ◽  
Field Isolates

ABSTRACT Currently available primary screens for the selection of candidate antileishmanial compounds are not ideal. These techniques are time-consuming, laborious, and difficult to scale and require macrophages, which limit their use for high-throughput screening. We have developed Leishmania donovani field isolates that constitutively express the firefly luciferase reporter gene (luc) as a part of an episomal vector. An excellent correlation between parasite number and luciferase activity was observed. luc expression was stable, even in the absence of drug selection, for 4 weeks. The transfectants were infective to macrophages, and intracellular amastigotes exhibited luciferase activity. The suitability of these recombinant field isolates for in vitro screening of antileishmanial drugs was established. The luciferase-expressing sodium stibogluconate-resistant cell lines offer a model for the screening of compounds for resistance. The system is in routine use at the Central Drug Research Institute, Lucknow, India, for high-throughput screening of newly synthesized compounds.

Sign in / Sign up

Export Citation Format

Share Document