scholarly journals Comprehensive comparative analysis of strand-specific RNA sequencing methods

2010 ◽  
Vol 7 (9) ◽  
pp. 709-715 ◽  
Author(s):  
Joshua Z Levin ◽  
Moran Yassour ◽  
Xian Adiconis ◽  
Chad Nusbaum ◽  
Dawn Anne Thompson ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Rna Sequencing

Abstract 3186: Comparative analysis of different total RNA sequencing approaches

2012 ◽  
Author(s):  
Christine J. Sumner ◽  
Daniela Munafo ◽  
Larry McReynolds ◽  
Brad Langhorst ◽  
Ping Liu ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Rna Sequencing ◽  
Total Rna

Abstract 4867: Comparative analysis of RNA sequencing methods for characterization of cancer transcriptomics

2015 ◽  
Author(s):  
Ryan P. Abo ◽  
Ling Lin ◽  
Samuel S. Hunter ◽  
Deniz N. Dolcen ◽  
Rachel R. Paquette ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Rna Sequencing

scholarly journals RNA-sequencing-based comparative analysis of human hepatic progenitor cells and their niche from alcoholic steatohepatitis livers

2017 ◽  
Vol 8 (11) ◽  
pp. e3164-e3164 ◽  
Author(s):  
An Ceulemans ◽  
Stefaan Verhulst ◽  
Matthias Van Haele ◽  
Olivier Govaere ◽  
Juan-Jose Ventura ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Progenitor Cells ◽  
Rna Sequencing ◽  
Hepatic Progenitor Cells ◽  
Alcoholic Steatohepatitis

scholarly journals Author Correction: Comprehensive comparative analysis of 5′-end RNA-sequencing methods

2018 ◽  
Vol 15 (12) ◽  
pp. 1126-1126
Author(s):  
Xian Adiconis ◽  
Adam L. Haber ◽  
Sean K. Simmons ◽  
Ami Levy Moonshine ◽  
Zhe Ji ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Rna Sequencing

scholarly journals A comparative transcriptional landscape of two castor cultivars obtained by single-molecule sequencing comparative analysis

2020 ◽  
Author(s):  
Wei Zhou ◽  
Yaxing Zhou ◽  
Guoli Zhu ◽  
Yun Wang ◽  
Zhibiao He ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Comparative Analysis ◽  
Alternative Splicing ◽  
Rna Sequencing ◽  
Transcriptome Sequencing ◽  
Full Length ◽  
Transcriptional Analysis ◽  
Sequencing Analysis ◽  
Short Read ◽  
Alternative Splicing Events

AbstractBackground and ObjectivesCastor (Ricinus communis L.) is an important non-edible oilseed crop. Lm type female strains and normal amphiprotic strains are important castor cultivars, and are mainly different in inflorescence structures and leaf shapes. To better understand the mechanisums underling these differences at the molecular level, we performed comparative transcriptional analysis.Materials and MethodsFull-length transcriptome sequencing and short-read RNA sequencing were employed.ResultsA total of 76,068 and 44,223 non-redundant transcripts were obtained from high-quality transcripts of Lm type female strains and normal amphiprotic strains, respectively. In Lm female strain and normal amphiprotic strains 51,613 and 20,152 alternative splicing events were found, respectively. There were 13,239 transcription factors identified from the full-length transcriptomes. Comparative analysis showed great different gene expression of common and unique transcription factors between the two cultivars. Meanwhile, functional analysis of isoform was conducted. Full-length sequences were used as a reference genome, and short-read RNA sequencing analysis was performed to conduct differential gene analysis. Furthermore, the function of DEGs were performed to annotation analysis.ConclusionsThe results revealed considerable difference and expression diversity between two cultivars, well beyond what was reported in previous studies, likely reflecting the differences in architecture between these two cultivars.HighlightUsing the full-length transcriptome sequencing technology, we performed comparative analysis of transcription factors of two castor cultivars, analyzed alternative splicing events, and identified their lncRNAs.

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