scholarly journals Photoactivatable mCherry for high-resolution two-color fluorescence microscopy

2009 ◽  
Vol 6 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Fedor V Subach ◽  
George H Patterson ◽  
Suliana Manley ◽  
Jennifer M Gillette ◽  
Jennifer Lippincott-Schwartz ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy

scholarly journals Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging

2014 ◽  
Vol 111 (48) ◽  
pp. 17164-17169 ◽  
Author(s):  
Jérôme Boulanger ◽  
Charles Gueudry ◽  
Daniel Münch ◽  
Bertrand Cinquin ◽  
Perrine Paul-Gilloteaux ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Incidence Angle ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence

Confocal fluorescence microscopy with the tandem scanning light microscope

1989 ◽  
Vol 94 (4) ◽  
pp. 617-624
Author(s):  
S.J. Wright ◽  
J.S. Walker ◽  
H. Schatten ◽  
C. Simerly ◽  
J.J. McCarthy ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Light Microscope ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Complex Structures ◽  
Charge Coupled Device ◽  
Confocal Fluorescence ◽  
Cooled Ccd ◽  
Cellular Components

Applications of the tandem scanning confocal microscope (TSM) to fluorescence microscopy and its ability to resolve fluorescent biological structures are described. The TSM, in conjunction with a cooled charge-coupled device (cooled CCD) and conventional epifluorescence light source and filter sets, provided high-resolution, confocal data, so that different fluorescent cellular components were distinguished in three dimensions within the same cell. One of the unique features of the TSM is the ability to image fluorochromes excited by ultraviolet light (e.g. Hoechst, DAPI) in addition to fluorescein and rhodamine. Since the illumination is dim, photobleaching is insignificant and prolonged viewing of living specimens is possible. Series of optical sections taken in the Z-axis with the TSM were reproduced as stereo images and three-dimensional reconstructions. These data show that the TSM is potentially a powerful tool in fluorescence microscopy for determining three-dimensional relationships of complex structures within cells labeled with multiple fluorochromes.

scholarly journals High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

2010 ◽  
Vol 1 (3) ◽  
pp. 791 ◽  
Author(s):  
Keisuke Isobe ◽  
Akira Suda ◽  
Hiroshi Hashimoto ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Multiphoton Process

Localization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry

2014 ◽  
Vol 59 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Bianca Kyriacou ◽  
Katie L. Moore ◽  
David Paterson ◽  
Martin D. de Jonge ◽  
Daryl L. Howard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Fluorescence Microscopy ◽  
Secondary Ion Mass Spectrometry ◽  
Rice Grain ◽  
X Ray ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

scholarly journals The Cellular Localization of the p42 and p46 Oligoadenylate Synthetase 1 Isoforms and Their Impact on Mitochondrial Respiration

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1122 ◽  
Author(s):  
Stig Skrivergaard ◽  
Monica Skou Jensen ◽  
Tine Breckling Rolander ◽  
Tram Bao Ngoc Nguyen ◽  
Amanda Bundgaard ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Cellular Localization ◽  
Immunoblot Analysis ◽  
Rnase L ◽  
Oligoadenylate Synthetase ◽  
Innate Response ◽  
Viral Pathogens ◽  
The Difference ◽  
Caax Motif

The importance of the IFN-induced oligoadenylate synthetase (OAS) proteins and the OAS/RNase L pathway in the innate response against viral pathogens is well-established, however the observed differences in anti-viral activity between the human OAS1 p46 and p42 isoforms are not fully understood. The protein expression of these isoforms is determined by the SNP rs10774671, either being an A or a G allele resulting in expression of either the p42 or the p46 isoform. Using fluorescence microscopy and immunoblot analysis of fractionated cell samples, we show here that the CaaX motif is of key importance to the cellular localization. The OAS1 p42 isoform is mainly located in the cytosol, whereas the p46 isoform with a C-terminal CaaX motif is translocated to membranous organelles, like the mitochondria. We furthermore observed differences between p42 and p46 in their effect on mitochondrial physiology using high resolution respirometry and fluorometry. Overexpression of OAS1 p42 and IFN-β treatment of HeLa cells (AA genotype) resulted in significantly increased respiration, which was not seen with p46 overexpression. The difference in subcellular localization and mitochondrial effect of these two OAS1 isoforms might help to explain the anti-viral mechanisms that differentiate these proteins.

scholarly journals Understanding the Biological Basis of Autofluorescence Imaging for Oral Cancer Detection: High-Resolution Fluorescence Microscopy in Viable Tissue

2008 ◽  
Vol 14 (8) ◽  
pp. 2396-2404 ◽  
Author(s):  
Ina Pavlova ◽  
Michelle Williams ◽  
Adel El-Naggar ◽  
Rebecca Richards-Kortum ◽  
Ann Gillenwater
Keyword(s):  
High Resolution ◽  
Oral Cancer ◽  
Fluorescence Microscopy ◽  
Cancer Detection ◽  
Autofluorescence Imaging ◽  
Biological Basis
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