scholarly journals Robust single-particle tracking in live-cell time-lapse sequences

2008 ◽  
Vol 5 (8) ◽  
pp. 695-702 ◽  
Author(s):  
Khuloud Jaqaman ◽  
Dinah Loerke ◽  
Marcel Mettlen ◽  
Hirotaka Kuwata ◽  
Sergio Grinstein ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Time Lapse ◽  
Single Particle Tracking ◽  
Live Cell

scholarly journals Study of Wound Healing Dynamics by Single Pseudo-Particle Tracking in Phase Contrast Images Acquired in Time-Lapse

Entropy ◽  
2021 ◽  
Vol 23 (3) ◽  
pp. 284
Author(s):  
Riccardo Scheda ◽  
Silvia Vitali ◽  
Enrico Giampieri ◽  
Gianni Pagnini ◽  
Isabella Zironi
Keyword(s):  
Wound Healing ◽  
Single Cell ◽  
Particle Tracking ◽  
Single Particle ◽  
Phase Contrast ◽  
Time Lapse ◽  
Single Particle Tracking ◽  
Stochastic Motion ◽  
Wound Edge ◽  
Scratch Assay

Cellular contacts modify the way cells migrate in a cohesive group with respect to a free single cell. The resulting motion is persistent and correlated, with cells’ velocities self-aligning in time. The presence of a dense agglomerate of cells makes the application of single particle tracking techniques to define cells dynamics difficult, especially in the case of phase contrast images. Here, we propose an original pipeline for the analysis of phase contrast images of the wound healing scratch assay acquired in time-lapse, with the aim of extracting single particle trajectories describing the dynamics of the wound closure. In such an approach, the membrane of the cells at the border of the wound is taken as a unicum, i.e., the wound edge, and the dynamics is described by the stochastic motion of an ensemble of points on such a membrane, i.e., pseudo-particles. For each single frame, the pipeline of analysis includes: first, a texture classification for separating the background from the cells and for identifying the wound edge; second, the computation of the coordinates of the ensemble of pseudo-particles, chosen to be uniformly distributed along the length of the wound edge. We show the results of this method applied to a glioma cell line (T98G) performing a wound healing scratch assay without external stimuli. We discuss the efficiency of the method to assess cell motility and possible applications to other experimental layouts, such as single cell motion. The pipeline is developed in the Python language and is available upon request.

scholarly journals Lysosome Mobility Probed with Live Cell Imaging and Single Particle Tracking

2013 ◽  
Vol 104 (2) ◽  
pp. 651a
Author(s):  
Debjyoti Bandyopadhyay ◽  
Youngeun J. Kim ◽  
Jairo Zapata ◽  
Christine K. Payne
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Single Particle Tracking ◽  
Live Cell

scholarly journals Live‐cell imaging of octaarginine‐modified polymer dots via single particle tracking

2019 ◽  
Vol 52 (2) ◽  
pp. e12556 ◽  
Author(s):  
Yao Luo ◽  
Yuping Han ◽  
Xingjie Hu ◽  
Min Yin ◽  
Changfeng Wu ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Modified Polymer ◽  
Polymer Dots

scholarly journals Photoactivation of silicon rhodamines via a light-induced protonation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Michelle S. Frei ◽  
Philipp Hoess ◽  
Marko Lampe ◽  
Bianca Nijmeijer ◽  
Moritz Kueblbeck ◽  
...  
Keyword(s):  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Spectroscopic Properties ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Localization Microscopy ◽  
Super Resolution Microscopy ◽  
Single Molecule Localization Microscopy

Abstract Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Live-cell imaging and single-particle tracking of polyplex internalization

2010 ◽  
Vol 15 (23-24) ◽  
pp. 1093-1093
Author(s):  
Nadia Ruthardt ◽  
Karla de Bruin ◽  
Kevin Braeckmans ◽  
Ernst Wagner ◽  
Christoph Bräuchle
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Single Particle Tracking ◽  
Live Cell
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