scholarly journals Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche

2016 ◽  
Vol 16 (4) ◽  
pp. 419-425 ◽  
Author(s):  
Yue Shao ◽  
Kenichiro Taniguchi ◽  
Katherine Gurdziel ◽  
Ryan F. Townshend ◽  
Xufeng Xue ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Self Organized

scholarly journals Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix

2013 ◽  
Vol 110 (31) ◽  
pp. 12601-12606 ◽  
Author(s):  
S. Kusuma ◽  
Y.-I. Shen ◽  
D. Hanjaya-Putra ◽  
P. Mali ◽  
L. Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Vascular Networks ◽  
Self Organized ◽  
Synthetic Matrix

scholarly journals Direct synthesis of self-organized blastocyst-like cysts derived from human pluripotent stem cells

2019 ◽  
Author(s):  
Xiaopeng Wen ◽  
Shiho Terada ◽  
Koki Yoshimoto ◽  
Ken-ichiro Kamei
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Inner Cell Mass ◽  
Direct Synthesis ◽  
Cell Mass ◽  
Human Pluripotent Stem Cells ◽  
Molecular Signatures ◽  
Poly Ethylene Glycol ◽  
Self Organized ◽  
Poly Ethylene

AbstractWe introduce a simple, robust and scalable method to generate self-organized blastocyst-like cysts (soBLCs) from human pluripotent stem cells (hPSCs). We use a copolymer hydrogel of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG). hPSC aggregates with a diameter of approximately 117.2 ± 5.1 µm are cultured in a medium supplemented with a hydrogel and a serum for three days. Molecular signatures in the medium revealed the generation of trophoblasts and inner cell mass at specific positions in the soBLCs.

scholarly journals An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells

2017 ◽  
Vol 216 (12) ◽  
pp. 3981-3990 ◽  
Author(s):  
Kenichiro Taniguchi ◽  
Yue Shao ◽  
Ryan F. Townshend ◽  
Chari L. Cortez ◽  
Clair E. Harris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
De Novo ◽  
Time Lapse ◽  
Human Pluripotent Stem Cells ◽  
Human Embryos ◽  
Multicellular Aggregate ◽  
Self Organized ◽  
Time Lapse Imaging ◽  
Multiple Cells

Human pluripotent stem cells (hPSCs) self-organize into apicobasally polarized cysts, reminiscent of the lumenal epiblast stage, providing a model to explore key morphogenic processes in early human embryos. Here, we show that apical polarization begins on the interior of single hPSCs through the dynamic formation of a highly organized perinuclear apicosome structure. The membrane surrounding the apicosome is enriched in apical markers and displays microvilli and a primary cilium; its lumenal space is rich in Ca2+. Time-lapse imaging of isolated hPSCs reveals that the apicosome forms de novo in interphase, retains its structure during mitosis, is asymmetrically inherited after mitosis, and relocates to the recently formed cytokinetic plane, where it establishes a fully polarized lumen. In a multicellular aggregate of hPSCs, intracellular apicosomes from multiple cells are trafficked to generate a common lumenal cavity. Thus, the apicosome is a unique preassembled apical structure that can be rapidly used in single or clustered hPSCs to initiate self-organized apical polarization and lumenogenesis.

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial

326-LB: BCL-xL/BCL2L1 Is a Critical Anti-Apoptotic Protein that Suppresses BAK to Promote Pancreatic Specification from Human Pluripotent Stem Cells

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 326-LB
Author(s):  
LARRY SAI WENG LOO ◽  
ADRIAN TEO ◽  
SOUMITA GHOSH ◽  
ANDREAS ALVIN PURNOMO SOETEDJO ◽  
LINH NGUYEN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Apoptotic Protein
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