scholarly journals Self-assembled RNA interference microsponges for efficient siRNA delivery

2012 ◽  
Vol 11 (4) ◽  
pp. 316-322 ◽  
Author(s):  
Jong Bum Lee ◽  
Jinkee Hong ◽  
Daniel K. Bonner ◽  
Zhiyong Poon ◽  
Paula T. Hammond
Keyword(s):  
Rna Interference ◽  
Sirna Delivery ◽  
Self Assembled

Solvent-driven, self-assembled acid-responsive poly(ketalized serine)/siRNA complexes for RNA interference

2020 ◽  
Vol 8 (23) ◽  
pp. 6718-6729
Author(s):  
Shirley Wong ◽  
Jessica A. Kemp ◽  
Min Suk Shim ◽  
Young Jik Kwon
Keyword(s):  
Rna Interference ◽  
Nucleic Acid ◽  
Sirna Delivery ◽  
Endosomal Escape ◽  
Self Assembled

Self-assembled, biocompatible poly(kSer)/siRNA complexes demonstrate efficient nucleic acid encapsulation, internalization, endosomal escape, and acid-triggered cargo release, tackling multiple hurdles in siRNA delivery.

Nanostructured Hyaluronic Acid-based Materials for the Delivery of siRNA

2018 ◽  
Vol 24 (23) ◽  
pp. 2678-2691 ◽  
Author(s):  
Keval Shah ◽  
Sunita Chawla ◽  
Anuradha Gadeval ◽  
Goutham Reddy ◽  
Rahul Maheshwari ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Rna Interference ◽  
Sirna Delivery ◽  
Treatment Strategies ◽  
Aqueous Solubility ◽  
Micro Rna ◽  
Target Space ◽  
Significant Progress ◽  
Gene Therapies ◽  
Future Potential

Background: The search for the effective treatment strategies to combat a disease that is characterized by abnormal cell growth and known as cancer is still required to reach its destiny. To address the problem, recently several gene therapies based on novel RNA interference (RNAi) have been proposed such as siRNA, micro RNA, shRNA, etc. out of which, siRNAs (silencing RNA) promises to show significant progress in pharmacotherapy, including considerable expansion of the druggable target space and the possibility of treating cancer. Methods: This review aims to uncover the hyaluronic acid (HA) and HA-hybridized nanoplatforms for siRNA delivery systems with a particular focus on the discussion of available reports while addressing the future potential of HA-based treatment strategies. Results: HA modified siRNA delivery, as promised, provided better targeting potential in many types of cancers. In addition, it was able to modify the release of siRNA as well. Toxicity of HA is well mentioned however, the loophole is yet to be filled by exploring various remedies for overcoming toxicity. Conclusion: To overcome the problems associated with these emerging genetic tools, investigators have employed glycosaminoglycan HA-based biopolymers. This biopolymer offers a variety of properties such as biodegradability, biocompatibility, aqueous solubility, viscoelasticity, and non-immunogenicity.

scholarly journals Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery

2012 ◽  
Vol 7 (6) ◽  
pp. 389-393 ◽  
Author(s):  
Hyukjin Lee ◽  
Abigail K. R. Lytton-Jean ◽  
Yi Chen ◽  
Kevin T. Love ◽  
Angela I. Park ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sirna Delivery ◽  
Self Assembled

scholarly journals Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference

2018 ◽  
Vol 115 (12) ◽  
pp. E2696-E2705 ◽  
Author(s):  
Jiahe Li ◽  
Connie Wu ◽  
Wade Wang ◽  
Yanpu He ◽  
Elad Elkayam ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Rna Interference ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Effector Protein ◽  
Side Group ◽  
Argonaute 2 ◽  
Fundamental Research ◽  
Delivery Vehicles ◽  
Interfering Rna

Small interfering RNA (siRNA) represents a promising class of inhibitors in both fundamental research and the clinic. Numerous delivery vehicles have been developed to facilitate siRNA delivery. Nevertheless, achieving highly potent RNA interference (RNAi) toward clinical translation requires efficient formation of RNA-induced gene-silencing complex (RISC) in the cytoplasm. Here we coencapsulate siRNA and the central RNAi effector protein Argonaute 2 (Ago2) via different delivery carriers as a platform to augment RNAi. The physical clustering between siRNA and Ago2 is found to be indispensable for enhanced RNAi. Moreover, by utilizing polyamines bearing the same backbone but distinct cationic side-group arrangements of ethylene diamine repeats as the delivery vehicles, we find that the molecular structure of these polyamines modulates the degree of siRNA/Ago2-mediated improvement of RNAi. We apply this strategy to silence the oncogene STAT3 and significantly prolong survival in mice challenged with melanoma. Our findings suggest a paradigm for RNAi via the synergistic coassembly of RNA with helper proteins.

scholarly journals Self-Assembled Cationic β-Cyclodextrin Nanostructures for siRNA Delivery

2019 ◽  
Vol 16 (3) ◽  
pp. 1358-1366 ◽  
Author(s):  
Raghvendra Pratap Singh ◽  
Tania Hidalgo ◽  
Pierre-Andre Cazade ◽  
Raphael Darcy ◽  
Michael F. Cronin ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Self Assembled

scholarly journals Sticky Nanoparticles: A Platform for siRNA Delivery by a Bis(zinc(II) dipicolylamine)-Functionalized, Self-Assembled Nanoconjugate

2011 ◽  
Vol 51 (2) ◽  
pp. 445-449 ◽  
Author(s):  
Gang Liu ◽  
Ki Young Choi ◽  
Ashwinkumar Bhirde ◽  
Magdalena Swierczewska ◽  
Juan Yin ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Self Assembled

scholarly journals RNA interference-based technology: what role in animal agriculture?

2017 ◽  
Vol 57 (1) ◽  
pp. 1 ◽  
Author(s):  
B. J. Bradford ◽  
C. A. Cooper ◽  
M. L. Tizard ◽  
T. J. Doran ◽  
T. M. Hinton
Keyword(s):  
Rna Interference ◽  
Genome Engineering ◽  
Sirna Delivery ◽  
Protein Product ◽  
Target Cells ◽  
Shrna Expression ◽  
Animal Agriculture ◽  
Transgenic Expression ◽  
Regulatory Processes ◽  
Adverse Environmental Conditions

Animal agriculture faces a broad array of challenges, ranging from disease threats to adverse environmental conditions, while attempting to increase productivity using fewer resources. RNA interference (RNAi) is a biological phenomenon with the potential to provide novel solutions to some of these challenges. Discovered just 20 years ago, the mechanisms underlying RNAi are now well described in plants and animals. Intracellular double-stranded RNA triggers a conserved response that leads to cleavage and degradation of complementary mRNA strands, thereby preventing production of the corresponding protein product. RNAi can be naturally induced by expression of endogenous microRNA, which are critical in the regulation of protein synthesis, providing a mechanism for rapid adaptation of physiological function. This endogenous pathway can be co-opted for targeted RNAi either through delivery of exogenous small interfering RNA (siRNA) into target cells or by transgenic expression of short hairpin RNA (shRNA). Potentially valuable RNAi targets for livestock include endogenous genes such as developmental regulators, transcripts involved in adaptations to new physiological states, immune response mediators, and also exogenous genes such as those encoded by viruses. RNAi approaches have shown promise in cell culture and rodent models as well as some livestock studies, but technical and market barriers still need to be addressed before commercial applications of RNAi in animal agriculture can be realised. Key challenges for exogenous delivery of siRNA include appropriate formulation for physical delivery, internal transport and eventual cellular uptake of the siRNA; additionally, rigorous safety and residue studies in target species will be necessary for siRNA delivery nanoparticles currently under evaluation. However, genomic incorporation of shRNA can overcome these issues, but optimal promoters to drive shRNA expression are needed, and genetic engineering may attract more resistance from consumers than the use of exogenous siRNA. Despite these hurdles, the convergence of greater understanding of RNAi mechanisms, detailed descriptions of regulatory processes in animal development and disease, and breakthroughs in synthetic chemistry and genome engineering has created exciting possibilities for using RNAi to enhance the sustainability of animal agriculture.

Efficient Self‐Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in v itro

2019 ◽  
Vol 37 (6) ◽  
pp. 588-592 ◽  
Author(s):  
Qian Yao ◽  
Yuqi Chen ◽  
Fan Wu ◽  
Fan Wu ◽  
Chaoxing Liu ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Sirna Delivery ◽  
Rolling Circle ◽  
Dna Nanoparticles ◽  
Self Assembled
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