Ex vivo induction and expansion of antigen-specific cytotoxic T cells by HLA-Ig–coated artificial antigen-presenting cells

10.1038/nm869 ◽  
2003 ◽  
Vol 9 (5) ◽  
pp. 619-625 ◽  
Author(s):  
Mathias Oelke ◽  
Marcela V. Maus ◽  
Dominic Didiano ◽  
Carl H. June ◽  
Andreas Mackensen ◽  
...  
Keyword(s):  
T Cells ◽  
Ex Vivo ◽  
Cytotoxic T Cells ◽  
Antigen Presenting Cells ◽  
Artificial Antigen ◽  
Antigen Presenting

scholarly journals Selective tumor antigen vaccine delivery to human CD169+antigen-presenting cells using ganglioside-liposomes

2020 ◽  
Vol 117 (44) ◽  
pp. 27528-27539
Author(s):  
Alsya J. Affandi ◽  
Joanna Grabowska ◽  
Katarzyna Olesek ◽  
Miguel Lopez Venegas ◽  
Arnaud Barbaria ◽  
...  
Keyword(s):  
T Cells ◽  
Tumor Antigen ◽  
Tumor Antigens ◽  
Ex Vivo ◽  
Antigen Presenting Cells ◽  
Dependent Manner ◽  
Cross Presentation ◽  
Vaccine Carrier ◽  
Antigen Presenting

Priming of CD8+T cells by dendritic cells (DCs) is crucial for the generation of effective antitumor immune responses. Here, we describe a liposomal vaccine carrier that delivers tumor antigens to human CD169/Siglec-1+antigen-presenting cells using gangliosides as targeting ligands. Ganglioside-liposomes specifically bound to CD169 and were internalized by in vitro-generated monocyte-derived DCs (moDCs) and macrophages and by ex vivo-isolated splenic macrophages in a CD169-dependent manner. In blood, high-dimensional reduction analysis revealed that ganglioside-liposomes specifically targeted CD14+CD169+monocytes and Axl+CD169+DCs. Liposomal codelivery of tumor antigen and Toll-like receptor ligand to CD169+moDCs and Axl+CD169+DCs led to cytokine production and robust cross-presentation and activation of tumor antigen-specific CD8+T cells. Finally, Axl+CD169+DCs were present in cancer patients and efficiently captured ganglioside-liposomes. Our findings demonstrate a nanovaccine platform targeting CD169+DCs to drive antitumor T cell responses.

scholarly journals Scaffolds that mimic antigen-presenting cells enable ex vivo expansion of primary T cells

2018 ◽  
Vol 36 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Alexander S Cheung ◽  
David K Y Zhang ◽  
Sandeep T Koshy ◽  
David J Mooney
Keyword(s):  
T Cells ◽  
Ex Vivo ◽  
Antigen Presenting Cells ◽  
Ex Vivo Expansion ◽  
Antigen Presenting
Sign in / Sign up

Export Citation Format

Share Document