Long-term survival of neonatal porcine islets in nonhuman primates by targeting costimulation pathways

2006 ◽  
Vol 12 (3) ◽  
pp. 304-306 ◽  
Author(s):  
Kenneth Cardona ◽  
Gregory S Korbutt ◽  
Zvonimir Milas ◽  
James Lyon ◽  
Jose Cano ◽  
...  
Keyword(s):  
Nonhuman Primates ◽  
Term Survival ◽  
Long Term Survival ◽  
Porcine Islets ◽  
Neonatal Porcine Islets

scholarly journals Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits

2013 ◽  
Vol 2 (2) ◽  
pp. 101 ◽  
Author(s):  
Rafael ValdsGonzlez ◽  
Ana RodriguezVentura ◽  
Briceyda GonzlezRamrez ◽  
Benjamn LenMancilla ◽  
Pedro Valencia ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Term Survival ◽  
Long Term Survival ◽  
Porcine Islets ◽  
Neonatal Porcine Islets

(2) Targeting the T-cell costimulation pathways allows long-term survival of neonatal porcine islets in diabetic non-human primates

2007 ◽  
Vol 14 (2) ◽  
pp. 178-179 ◽  
Author(s):  
Kenneth Cardona ◽  
Elizabeth Strobert ◽  
Ray V. Rajotte ◽  
Thomas C. Pearson ◽  
Christian P. Larsen
Keyword(s):  
T Cell ◽  
Term Survival ◽  
Long Term Survival ◽  
Porcine Islets ◽  
Neonatal Porcine Islets

Prolonged Survival of Microencapsulated Neonatal Porcine Islet Xenografts in Immune-Competent Mice without Antirejection Therapy

2008 ◽  
Vol 17 (10-11) ◽  
pp. 1243-1256 ◽  
Author(s):  
Tsunehiro Kobayashi ◽  
Hossein Arefanian ◽  
George Harb ◽  
Eric B. Tredget ◽  
Ray V. Rajotte ◽  
...  
Keyword(s):  
Islet Graft ◽  
Term Survival ◽  
Long Term Survival ◽  
Porcine Islet ◽  
In Vivo Culture ◽  
Neonatal Porcine Islets ◽  
Deficient Mice

Several studies have demonstrated that in vitro culture of islets prolonged islet graft survival in immune-competent mice without administration of antirejection drugs. However, we recently showed that in vitro cultured microencapsulated neonatal porcine islets (NPI) were rejected in immune-competent mice not receiving antirejection therapy. The aim of this study was to determine whether culture of microencapsulated NPI in vivo could promote long-term survival of microencapsulated NPI in immune-competent mice without administration of antirejection drugs. Microencapsulated NPI that were cultured in vitro for 7 and 50 days or transplanted initially in immune-deficient C.B.-17 SCID-BEIGE mice for 100 days (in vivo cultured) were characterized and transplanted into streptozotocin-induced diabetic immune-competent BALB/c mice. Day 50 in vitro cultured and day 100 in vivo cultured microencapsulated NPI showed significantly higher insulin and DNA content, indicating maturation of NPI compared to day 7 in vitro cultured microencapsulated NPI. Interestingly, in vivo cultured microencapsulated NPI expressed lower levels of porcine antigens compared to day 7 and day 50 in vitro cultured microencapsulated NPI. Transplantation of day 7 in vitro cultured microencapsulated NPI did not reverse diabetes in immune-competent BALB/c mouse recipients. In contrast, transplantation of day 50 in vitro cultured and in vivo cultured microencapsulated NPI into diabetic immune-competent BALB/c mice resulted in the immediate reversal of hyperglycemia within 2 days posttransplantation. However, all recipients of day 50 in vitro cultured microencapsulated NPI eventually rejected their grafts by day 15 posttransplantation, while 6 of 10 BALB/c mouse recipients of in vivo cultured microencapsulated NPI maintained normoglycemia for 100 days posttransplantation. These results show that in vivo culture of NPI in immune-deficient mice results in the modulation of NPI, which allows for their long-term survival in immune-competent mice without antirejection therapy.

LONG TERM SURVIVAL AND FUNCTION OF ADULT PORCINE ISLETS CO-TRANSPLANTED WITH ADULT PORCINE SERTOLI CELLS INTO RATS WITHOUT IMMUNOSUPPRESSION.

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 91-92 ◽  
Author(s):  
D J.G. White ◽  
C Hasilo ◽  
G Vilk ◽  
D M. Mazzuca ◽  
A Pepper ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Term Survival ◽  
Long Term Survival ◽  
Porcine Islets ◽  
And Function
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