ISDoT: in situ decellularization of tissues for high-resolution imaging and proteomic analysis of native extracellular matrix

2017 ◽  
Vol 23 (7) ◽  
pp. 890-898 ◽  
Author(s):  
Alejandro E Mayorca-Guiliani ◽  
Chris D Madsen ◽  
Thomas R Cox ◽  
Edward R Horton ◽  
Freja A Venning ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
High Resolution ◽  
Proteomic Analysis ◽  
High Resolution Imaging ◽  
Resolution Imaging

Development of a translation stage for in situ noninvasive analysis and high-resolution imaging

inArt 2016 ◽  
2017 ◽  
pp. 185-189
Author(s):  
David Strivay ◽  
Mathieu Clar ◽  
Said Rakkaa ◽  
Francois-Philippe Hocquet ◽  
Catherine Defeyt
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Noninvasive Analysis ◽  
Translation Stage ◽  
Resolution Imaging

High resolution imaging of mitochondrial membranes by in situ atomic force microscopy

RSC Advances ◽  
2013 ◽  
Vol 3 (3) ◽  
pp. 708-712 ◽  
Author(s):  
Yongmei Tian ◽  
Jiahan Li ◽  
Mingjun Cai ◽  
Weidong Zhao ◽  
Haijiao Xu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Mitochondrial Membranes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Resolution Imaging

Improved structural preservation of high-pressure frozen cartilage

1993 ◽  
Vol 51 ◽  
pp. 108-109
Author(s):  
Daniel Studer ◽  
Jeannine Wagner ◽  
Ernst B. Hunziker
Keyword(s):  
Extracellular Matrix ◽  
High Pressure ◽  
High Resolution ◽  
Growth Plate ◽  
High Resolution Imaging ◽  
Chemical Fixation ◽  
Growth Plate Cartilage ◽  
Rat Growth ◽  
Resolution Imaging ◽  
Structural Preservation

Adult cartilage is a unique tissue in that it is avascular and lacks innervation. The chondrocytes are embedded in an extracellular matrix which in most cases occupies 60 to 90% of the tissue volume. The ultrastructure and composition of rat growth plate cartilage has been investigated (1). It was found that during conventional chemical fixation of me tissue (2), about 60% of the extracellular matrix proteoglycans were lost (3). This artefact can be prevented if proteoglycans are precipitated by cationic dyes (eg. ruthenium hexaamine trichloride) during fixation (4) which forms relatively large precipitates by rendering high resolution imaging impossible. High pressure frozen extracellular matrix revealed a fine meshwork which was attributed to proteoglycan distribution (5). A meshwork due to segregation during freezing was not seen in recent experiments where adaequately frozen cartilage was investigated.200μm thick sections of rat growth plate cartilage were excised in a bath of hexadecene (6). The sections were again processed under hexadecene, firstly punched (diameter 1.7mm) and then transferred to an aluminum sandwhich (corresponding in size to the sample).

In situ, high-resolution imaging of labile phosphorus in sediments of a large eutrophic lake

2015 ◽  
Vol 74 ◽  
pp. 100-109 ◽  
Author(s):  
Shiming Ding ◽  
Chao Han ◽  
Yanping Wang ◽  
Lei Yao ◽  
Yan Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Eutrophic Lake ◽  
High Resolution Imaging ◽  
Labile Phosphorus ◽  
Resolution Imaging

Development of a translation stage for in situ noninvasive analysis and high-resolution imaging

2016 ◽  
Vol 122 (11) ◽  
Author(s):  
David Strivay ◽  
Mathieu Clar ◽  
Said Rakkaa ◽  
Francois-Philippe Hocquet ◽  
Catherine Defeyt
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Noninvasive Analysis ◽  
Translation Stage ◽  
Resolution Imaging
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