scholarly journals JNK1 negatively controls antifungal innate immunity by suppressing CD23 expression

2017 ◽  
Vol 23 (3) ◽  
pp. 337-346 ◽  
Author(s):  
Xueqiang Zhao ◽  
Yahui Guo ◽  
Changying Jiang ◽  
Qing Chang ◽  
Shilei Zhang ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Cd23 Expression

scholarly journals CD23 Mediates Innate Immunity Against Aspergillus Fumigatus Infection in Human Alveolar Macrophages Through Activation by PU.1

2021 ◽  
Author(s):  
Min Wang ◽  
Ming Zhang ◽  
Jiayong Qiu ◽  
Chenyang Liu ◽  
Yao Lou ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Aspergillus Fumigatus ◽  
Alveolar Macrophages ◽  
Inflammatory Markers ◽  
Inflammatory Marker ◽  
Mouse Lung ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lectin Receptors ◽  
Human Alveolar Macrophages ◽  
Cd23 Expression

Abstract Aspergillosis is a common cause of morbidity and mortality in immunocompromised populations. CD23 is a novel C-type lectin receptors (CLR) recognizing α-mannan and β-glucan in the cell wall of Aspergillus fumigatus (AF) that exerts a host innate immune response. However, the molecular mechanism underlying CD23 mediating immunity against AF infection in human alveolar macrophages is still unclear. In this study, we detected the expression of CD23 and PU.1 and the inflammatory markers IL-1β, IL-6, TNF-α and IL-10 by qRT–PCR, Western blotting and enzyme linked immunosorbent assay (ELISA) analysis in human alveolar macrophages (AMs) with AF infection. Phagocytosis of macrophages with altered CD23 expression and histological changes in lung tissues transfected with CD23-expressing adenoviruses in AF infection were investigated. Dual luciferase, chromatin immunoprecipitation assay (ChIP) and electrophoretic mobility shift assay (EMSA) was performed to detect the interaction of PU.1 and CD23. The results showed that the expression of CD23, PU.1 and these inflammatory markers increased significantly with the time of AF infection. Increasing CD23 expression strengthened the phagocytosis of AMs, and exogenous CD23 attenuated pathological defects in immunodeficient mouse lung tissues with AF infection. Moreover, CD23 was directly activated by PU.1. PU.1 siRNA resulted in downregulation of inflammatory marker expression, but overexpression of CD23 significantly increased the expression of these markers. Our study concluded that CD23 mediates innate immunity against AF in human AMs through activation of PU.1. Therefore, PU.1/CD23 may be a new anti-aspergillosis therapeutic for the treatment of invasive aspergillosis with the deepening of gene therapy and its wide application in the clinic.

DNA Makes RNA Makes Innate Immunity

2009 ◽  
Vol 138 (33) ◽  
pp. 428-430
Author(s):  
Luke A.J. O'Neill
Keyword(s):  
Innate Immunity

Alpha-Amylase/Trypsin Inhibitors, novel activators of innate immunity in ex-vivo tissue explants

2013 ◽  
Vol 51 (08) ◽  
Author(s):  
V Zevallos ◽  
P Olinga ◽  
Y Junker ◽  
PB Tung ◽  
N Volz ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Ex Vivo ◽  
Trypsin Inhibitors ◽  
Alpha Amylase ◽  
Tissue Explants ◽  
Ex Vivo Tissue

Hippo signaling counter regulates early innate immunity in hepatocytes exposed to Hepatitis B virus

2020 ◽  
Author(s):  
X Luo ◽  
M Lu ◽  
HA Baba ◽  
G Gerken ◽  
H Wedemeyer ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hippo Signaling ◽  
B Virus

Polysaccharides from Plantaginaceae and Brassicaceae: effects on innate immunity

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
A Miron ◽  
CE Lupusoru ◽  
M Pavelescu ◽  
M Hancianu ◽  
U Stanescu ◽  
...  
Keyword(s):  
Innate Immunity
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