Hypersensitive site 7 of the TH2 locus control region is essential for expressing TH2 cytokine genes and for long-range intrachromosomal interactions

2004 ◽  
Vol 6 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Gap Ryol Lee ◽  
Charalampos G Spilianakis ◽  
Richard A Flavell
Keyword(s):  
Long Range ◽  
Control Region ◽  
Locus Control Region ◽  
Hypersensitive Site ◽  
Cytokine Genes ◽  
Th2 Cytokine

scholarly journals Hypersensitive site 6 of the Th2 locus control region is essential for Th2 cytokine expression

2013 ◽  
Vol 110 (17) ◽  
pp. 6955-6960 ◽  
Author(s):  
A. Williams ◽  
G. R. Lee ◽  
C. G. Spilianakis ◽  
S. S. Hwang ◽  
S. C. Eisenbarth ◽  
...  
Keyword(s):  
Control Region ◽  
Locus Control Region ◽  
Cytokine Expression ◽  
Hypersensitive Site ◽  
Th2 Cytokine

scholarly journals Locus Control Region Transcription Plays an Active Role in Long-Range Gene Activation

2006 ◽  
Vol 23 (4) ◽  
pp. 619
Author(s):  
Yugong Ho ◽  
Felice Elefant ◽  
Stephen A. Liebhaber ◽  
Nancy E. Cooke
Keyword(s):  
Long Range ◽  
Control Region ◽  
Gene Activation ◽  
Locus Control Region ◽  
Active Role

scholarly journals Requirement of an E1A-sensitive Coactivator for Long-range Transactivation by the β-Globin Locus Control Region

1999 ◽  
Vol 274 (38) ◽  
pp. 26850-26859 ◽  
Author(s):  
E. Camilla Forsberg ◽  
Kirby Johnson ◽  
Tatiana N. Zaboikina ◽  
Eric A. Mosser ◽  
Emery H. Bresnick
Keyword(s):  
Long Range ◽  
Control Region ◽  
Locus Control Region

scholarly journals Hypersensitive site 4 of the human β globin locus control region

1991 ◽  
Vol 19 (7) ◽  
pp. 1413-1419 ◽  
Author(s):  
Sara Pruzina ◽  
Olivia Hanscombe ◽  
David Whyatt ◽  
Frank Grosveld ◽  
Sjaak Philipsen
Keyword(s):  
Control Region ◽  
Locus Control Region ◽  
Hypersensitive Site

Direct interaction of NF-E2 with hypersensitive site 2 of the β-globin locus control region in living cells

Blood ◽  
2000 ◽  
Vol 96 (1) ◽  
pp. 334-339 ◽  
Author(s):  
E. Camilla Forsberg ◽  
Karen M. Downs ◽  
Emery H. Bresnick
Keyword(s):  
Gene Expression ◽  
Control Region ◽  
Globin Gene ◽  
Locus Control Region ◽  
Erythroid Cell ◽  
Hypersensitive Site ◽  
Intact Cells ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Globin Gene Expression

The human β-globin locus control region (LCR) confers high-level, tissue-specific expression to the β-globin genes. Tandem Maf recognition elements (MAREs) within the hypersensitive site 2 (HS2) subregion of the LCR are important for the strong enhancer activity of the LCR. Multiple proteins are capable of interacting with these sites in vitro, including the erythroid cell- and megakaryocyte-specific transcription factor, NF-E2. The importance of NF-E2 for β-globin gene expression is evident in murine erythroleukemia cells lacking the p45 subunit of NF-E2. These CB3 cells have a severe defect in - and β-globin gene transcription, which can be restored by expression of NF-E2. However, mice nullizygous for p45 express nearly normal levels of β-globin. Thus, either a redundant factor(s) exists in mice that can functionally replace NF-E2, or NF-E2 does not function through the LCR to regulate β-globin gene expression. To address this issue, we asked whether NF-E2 binds directly to the tandem MAREs of HS2 in intact cells. Using a chromatin immunoprecipitation assay, we provide evidence for NF-E2 binding directly and specifically to HS2 in living erythroleukemia cells and in mouse fetal liver. The specific immunoisolation of HS2 sequences was dependent on the presence of p45 and on intact MAREs within HS2. These results support a direct role for NF-E2 in the regulation of β-globin gene expression through activation of the LCR.

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