Induction of T cell development and establishment of T cell competence from embryonic stem cells differentiated in vitro

2004 ◽  
Vol 5 (4) ◽  
pp. 410-417 ◽  
Author(s):  
Thomas M Schmitt ◽  
Renée F de Pooter ◽  
Matthew A Gronski ◽  
Sarah K Cho ◽  
Pamela S Ohashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
T Cell Development ◽  
Embryonic Stem ◽  
Cell Development ◽  
Cell Competence

scholarly journals Generation of Functional Thymic Epithelium from Human Embryonic Stem Cells that Supports Host T Cell Development

2013 ◽  
Vol 13 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Audrey V. Parent ◽  
Holger A. Russ ◽  
Imran S. Khan ◽  
Taylor N. LaFlam ◽  
Todd C. Metzger ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
T Cell Development ◽  
Embryonic Stem ◽  
Cell Development ◽  
Thymic Epithelium

3040 – IN VITRO RECAPITULATION OF MURINE T CELL DEVELOPMENT FROM SINGLE HEMATOPOIETIC STEM CELLS

2020 ◽  
Vol 88 ◽  
pp. S51
Author(s):  
Victoria Sun ◽  
Amelie Montel-Hagen ◽  
David Casero ◽  
Steven Tsai ◽  
Alexandre Zampieri ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Hematopoietic Stem Cells ◽  
T Cell Development ◽  
Cell Development ◽  
Hematopoietic Stem

Thymopentin enhances the generation of T-cell lineage derived from human embryonic stem cells in vitro

2015 ◽  
Vol 331 (2) ◽  
pp. 387-398 ◽  
Author(s):  
Ming-Xia Zhu ◽  
Wen-Li Wan ◽  
Hai-Shen Li ◽  
Jing Wang ◽  
Gui-An Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Lineage

Characterization in vitro and engraftment potential in vivo of human progenitor T cells generated from hematopoietic stem cells

Blood ◽  
2009 ◽  
Vol 114 (5) ◽  
pp. 972-982 ◽  
Author(s):  
Génève Awong ◽  
Elaine Herer ◽  
Charles D. Surh ◽  
John E. Dick ◽  
Ross N. La Motte-Mohs ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Hematopoietic Stem Cells ◽  
T Cell Development ◽  
Cell Development ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Hematopoietic Stem ◽  
Human T Cell

T-cell development follows a defined set of stage-specific differentiation steps. However, molecular and cellular events occurring at early stages of human T-cell development remain to be fully elucidated. To address this, human umbilical cord blood (UCB) hematopoietic stem cells (HSCs) were induced to differentiate to the T lineage in OP9-DL1 cocultures. A developmental program involving a sequential and temporally discrete expression of key differentiation markers was revealed. Quantitative clonal analyses demonstrated that CD34+CD38− and CD34+CD38lo subsets of UCB contain a similarly high T-lineage progenitor frequency, whereas the frequency in CD34+CD38+/hi cells was 5-fold lower. Delta-like/Notch-induced signals increased the T-cell progenitor frequency of CD34+CD38−/lo cells differentiated on OP9-DL1, and 2 distinct progenitor subsets, CD34+CD45RA+CD7++CD5−CD1a− (proT1) and CD34+CD45RA+CD7++CD5+CD1a− (proT2), were identified and their thymus engrafting capacity was examined, with proT2 cells showing a 3-fold enhanced reconstituting capacity compared with the proT1 subset. Furthermore, in vitro–generated CD34+CD7++ progenitors effectively engrafted the thymus of immunodeficient mice, which was enhanced by the addition of an IL-7/IL-7 antibody complex. Taken together, the identification of T-progenitor subsets readily generated in vitro may offer important avenues to improve cellular-based immune-reconstitution approaches.

scholarly journals In vitro post-meiotic germ cell development from human embryonic stem cells

2009 ◽  
Vol 24 (12) ◽  
pp. 3150-3159 ◽  
Author(s):  
B. Aflatoonian ◽  
L. Ruban ◽  
M. Jones ◽  
R. Aflatoonian ◽  
A. Fazeli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Development ◽  
Germ Cell Development
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