scholarly journals Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus

2016 ◽  
Vol 17 (9) ◽  
pp. 1102-1108 ◽  
Author(s):  
Wanwisa Dejnirattisai ◽  
Piyada Supasa ◽  
Wiyada Wongwiwat ◽  
Alexander Rouvinski ◽  
Giovanna Barba-Spaeth ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Zika Virus ◽  
Cross Reactivity ◽  
Antibody Dependent Enhancement

scholarly journals Cross-Reaction, Enhancement, and Neutralization Activity of Dengue Virus Antibodies against Zika Virus: A Study in the Mexican Population

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Mayra R. Montecillo-Aguado ◽  
Alfredo E. Montes-Gómez ◽  
Julio García-Cordero ◽  
Josselin Corzo-Gómez ◽  
Héctor Vivanco-Cid ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Acute Phase ◽  
Zika Virus ◽  
Neutralizing Antibodies ◽  
Cross Reactivity ◽  
Health Concern ◽  
Serum Samples ◽  
Zikv Infection ◽  
Antibody Dependent Enhancement ◽  
Homologous Antigen

Zika virus (ZIKV), an emerging mosquito-borne flavivirus, has quickly spread in many regions around the world where dengue virus (DENV) is endemic. This represents a major health concern, given the high homology between these two viruses, which can result in cross-reactivity. The aim of this study was to determine the cross-reacting antibody response of the IgM and IgG classes against the recombinant envelope protein of ZIKV (rE-ZIKV) in sera from patients with acute-phase infection of different clinical forms of dengue, i.e., dengue fever (DF) and dengue hemorrhagic fever (DHF) (before the arrival of ZIKV in Mexico 2010), as well as acute-phase sera of ZIKV patients, together with the implications in neutralization and antibody-dependent enhancement. Differences in IgM responses were observed in a number of DF and DHF patients whose sera cross-reacted with the rE-ZIK antigen, with 42% recognition between acute-phase DHF and ZIKV but 27% recognition between DF and ZIKV. Regarding IgG antibodies, 71.5% from the DF group showed cross-reactivity to rE-ZIKV in contrast with 50% and only 25% of DHF and ZIKV serum samples, respectively, which specifically recognized the homologous antigen. The DHF group showed more enhancement of ZIKV infection of FCRγ-expressing cells compared to the DF group. Furthermore, the DHF group also showed a higher cross-neutralizing ability than that of DF. This is the first report where DF and DHF serum samples were evaluated for cross-reactivity against Zika protein and ZIKV. Furthermore, DENV serum samples cross-protect against ZIKV through neutralizing antibodies but at the same time mediate antibody-dependent enhancement in the sequential ZIKV infection.

scholarly journals Dengue Virus and Zika Virus Serological Cross-reactivity and Their Impact on Pathogenesis in Mice

2018 ◽  
Vol 219 (2) ◽  
pp. 223-233 ◽  
Author(s):  
Satoru Watanabe ◽  
Nicole Wei Wen Tan ◽  
Kitti Wing Ki Chan ◽  
Subhash G Vasudevan
Keyword(s):  
Dengue Virus ◽  
Zika Virus ◽  
Cross Reactivity

scholarly journals Ability To Serologically Confirm Recent Zika Virus Infection in Areas with Varying Past Incidence of Dengue Virus Infection in the United States and U.S. Territories in 2016

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Nicole P. Lindsey ◽  
J. Erin Staples ◽  
Krista Powell ◽  
Ingrid B. Rabe ◽  
Marc Fischer ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Denv Infection ◽  
The United States ◽  
American Samoa ◽  
Cross Reactivity ◽  
Immunoglobulin M ◽  
Positive Results

ABSTRACTCross-reactivity within flavivirus antibody assays, produced by shared epitopes in the envelope proteins, can complicate the serological diagnosis of Zika virus (ZIKAV) infection. We assessed the utility of the plaque reduction neutralization test (PRNT) to confirm recent ZIKAV infections and rule out misleading positive immunoglobulin M (IgM) results in areas with various levels of past dengue virus (DENV) infection incidence. We reviewed PRNT results of sera collected for diagnosis of ZIKAV infection from 1 January through 31 August 2016 with positive ZIKAV IgM results, and ZIKAV and DENV PRNTs were performed. PRNT result interpretations included ZIKAV, unspecified flavivirus, DENV infection, or negative. For this analysis, ZIKAV IgM was considered false positive for samples interpreted as a DENV infection or negative. In U.S. states, 208 (27%) of 759 IgM-positive results were confirmed to be ZIKAV compared to 11 (21%) of 52 in the U.S. Virgin Islands (USVI), 15 (15%) of 103 in American Samoa, and 13 (11%) of 123 in Puerto Rico. In American Samoa and Puerto Rico, more than 80% of IgM-positive results were unspecified flavivirus infections. The false-positivity rate was 27% in U.S. states, 18% in the USVI, 2% in American Samoa, and 6% in Puerto Rico. In U.S. states, the PRNT provided a virus-specific diagnosis or ruled out infection in the majority of IgM-positive samples. Almost a third of ZIKAV IgM-positive results were not confirmed; therefore, providers and patients must understand that IgM results are preliminary. In territories with historically higher rates of DENV transmission, the PRNT usually could not differentiate between ZIKAV and DENV infections.

scholarly journals The inability of a dengue NS1 ELISA to detect Zika infections

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Monique da Rocha Queiroz Lima ◽  
Thais Chouin-Carneiro ◽  
Elzinandes Azeredo ◽  
Luciana Santos Barbosa ◽  
Thiara Manuele Alves Souza ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Dengue Virus ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
Signs And Symptoms ◽  
High Accuracy ◽  
Cross Reactivity ◽  
High Rate ◽  
Difficult Diagnosis ◽  
Capture Assay

AbstractThe presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here, we evaluated a Dengue NS1 capture assay for early and differential diagnosis of dengue during the Zika epidemic occurred in Brazil in 2016. Samples (n = 227) from 218 patients included sera, plasma and urine from previously confirmed acute cases of Zika, dengue and Zika/dengue co-infections. Nine of those patients presented two specimens. The Dengue NS1 test was very specific for dengue diagnosis (99.32%), even in the co-circulation with ZIKV, and exhibited a high accuracy in not detecting acute Zika infections (92.43%). Our findings showed that the dengue NS1 capture test analyzed here was not able to recognize the ZIKV NS1 and its potential for cross-reaction.

scholarly journals Development of Envelope Protein Antigens To Serologically Differentiate Zika Virus Infection from Dengue Virus Infection

2017 ◽  
Vol 56 (3) ◽  
Author(s):  
Lakshmanane Premkumar ◽  
Matthew Collins ◽  
Stephen Graham ◽  
Guei-Jiun Alice Liou ◽  
Cesar A. Lopez ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Envelope Protein ◽  
Population Level ◽  
Denv Infection ◽  
Cross Reactivity ◽  
Protein Antigens ◽  
Zikv Infection ◽  
Serological Tests

ABSTRACT Zika virus (ZIKV) is an emerging flavivirus that can cause birth defects and neurologic complications. Molecular tests are effective for diagnosing acute ZIKV infection, although the majority of infections produce no symptoms at all or present after the narrow window in which molecular diagnostics are dependable. Serology is a reliable method for detecting infections after the viremic period; however, most serological assays have limited specificity due to cross-reactive antibodies elicited by flavivirus infections. Since ZIKV and dengue virus (DENV) widely cocirculate, distinguishing ZIKV infection from DENV infection is particularly important for diagnosing individual cases or for surveillance to coordinate public health responses. Flaviviruses also elicit type-specific antibodies directed to non-cross-reactive epitopes of the infecting virus; such epitopes are attractive targets for the design of antigens for development of serological tests with greater specificity. Guided by comparative epitope modeling of the ZIKV envelope protein, we designed two recombinant antigens displaying unique antigenic regions on domain I (Z-EDI) and domain III (Z-EDIII) of the ZIKV envelope protein. Both the Z-EDI and Z-EDIII antigens consistently detected ZIKV-specific IgG in ZIKV-immune sera but not cross-reactive IgG in DENV-immune sera in late convalescence (>12 weeks postinfection). In contrast, during early convalescence (2 to 12 weeks postinfection), secondary DENV-immune sera and some primary DENV-immune sera cross-reacted with the Z-EDI and Z-EDIII antigens. Analysis of sequential samples from DENV-immune individuals demonstrated that Z-EDIII cross-reactivity peaked in early convalescence and declined steeply over time. The Z-EDIII antigen has much potential as a diagnostic antigen for population-level surveillance and for detecting past infections in patients.

scholarly journals Modulated Zika virus NS1 conjugate offers advantages for accurate detection of Zika virus specific antibody in double antigen binding and Ig capture enzyme immunoassays

2019 ◽  
Author(s):  
Richard S Tedder ◽  
Steve Dicks ◽  
Samreen Ijaz ◽  
Nathalia Caroline Santiago de Souza ◽  
Anderson Vincente de Paula ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Zika Virus ◽  
Cross Reactivity ◽  
Antigen Binding ◽  
Clinical Value ◽  
Serum Samples ◽  
Capture Assay ◽  
Homologous Antigen ◽  
Virus Serotype ◽  
Ns1 Antigen

AbstractThe accurate diagnosis and seroprevalence investigations of Zika virus (ZKV) infections remain complex due to cross reactivity with other flaviviruses. Two assay formats, both using labelled Zika virus NS1 antigen as a revealing agent (a double antigen binding assay, DABA, and an immunoglobulin Ig capture assay, IgG capture) were initially developed and compared with the indirect EuroimmunZ assay for the detection of anti-Zika antibody. Of 147 pre-Zika period serum samples, 39 (27%) were reactive in the EuroimmunZ or the DABA assays, 28 sera concordantly so. Such false reactivity was influenced by the serotype of Dengue virus (DV) to which individuals had been exposed to. Thus, of sera from patients undergoing secondary Dengue virus infection of known serotype, 91%, 45% and 28% of Dengue virus serotype 2, 3 and 4 respectively were reactive in one or more of the three assays. A novel method of quenching false sero-reactivity was therefore developed for the DABA and IgG capture assays. Initial addition of a single homologous Dengue virus serotype 3 NS1Ag quench significantly ablated false reactivities in the pre-Zika period sera. An equipotent quadrivalent quench comprising homologous Dengue virus serotypes 1 to 4 NS1Ag was shown to be optimum yet retained sensitivity for the detection of specific anti-Zika antibody. Comparing DABA and IgG capture assays using quenched and unquenched conjugates in comparison with EuroimmunZ early in the course of PCR-confirmed infection indicated that a significant component of the apparent early anti-ZIKA antibody response is likely to be due to a Zika virus-driven anamnestic anti-Dengue virus response. The increased specificity provided by homologous antigen quenching is likely to provide a significant improvement in sero-diagnostics and to be of clinical value.

scholarly journals T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus

2017 ◽  
Vol 2 (2) ◽  
pp. 274 ◽  
Author(s):  
Dominic Paquin-Proulx ◽  
Fabio E. Leal ◽  
Cassia G. Terrassani Silveira ◽  
Alvino Maestri ◽  
Claudia Brockmeyer ◽  
...  
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
Zika Virus ◽  
Cell Response ◽  
Cell Epitope ◽  
Denv Infection ◽  
Cross Reactivity ◽  
T Cell Response ◽  
Cd4 T Cell ◽  
T Cell Epitopes

Background: The outbreak of Zika virus (ZIKV) infection in Brazil has raised concerns that infection during pregnancy could cause microcephaly and other severe neurodevelopmental malformations in the fetus. The mechanisms by which ZIKV causes fetal abnormalities are largely unknown. The importance of pre-infection with dengue virus (DENV), or other flaviviruses endemic to Brazil, remains to be investigated. It has been reported that antibodies directed against DENV can increase ZIKV infectivity by antibody dependent enhancement (ADE), suggesting that a history of prior DENV infection might worsen the outcome of ZIKV infection.Methods: We used bioinformatics tools to design 18 peptides from the ZIKV envelope containing predicted HLA-I T-cell epitopes and investigated T-cell cross-reactivity between ZIKV-infected individuals and DENV-vaccinated subjects by IFNg ELISPOT.Results: Three peptides induced IFNg production in both ZIKV-infected subjects and in DENV-vaccinated individuals. Flow cytometry indicated that 1 ZIKV peptide induced a CD4+ T-cell response in DENV-vaccinated subjects.Conclusions: We demonstrated that vaccination against DENV induced a T-cell response against ZIKV and identified one such CD4+ T-cell epitope. The ZIKV-reactive CD4+ T cells induced by DENV vaccination and identified in this study could contribute to the appearance of cross-reactive antibodies mediating ADE.

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