scholarly journals Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus

2011 ◽  
Vol 12 (11) ◽  
pp. 1045-1054 ◽  
Author(s):  
Laurel A Monticelli ◽  
Gregory F Sonnenberg ◽  
Michael C Abt ◽  
Theresa Alenghat ◽  
Carly G K Ziegler ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Lung Tissue ◽  
Innate Lymphoid Cells ◽  
Tissue Homeostasis ◽  
Lymphoid Cells

scholarly journals Innate lymphoid cells in tissue homeostasis and diseases

2017 ◽  
Vol 9 (23) ◽  
pp. 979 ◽  
Author(s):  
Aline Ignacio ◽  
Cristiane Naffah Souza Breda ◽  
Niels Olsen Saraiva Camara
Keyword(s):  
Innate Lymphoid Cells ◽  
Tissue Homeostasis ◽  
Lymphoid Cells

scholarly journals Optimized Protocol for Characterization of Mouse Gut Innate Lymphoid Cells

2020 ◽  
Vol 11 ◽  
Author(s):  
Ana Valle-Noguera ◽  
María José Gómez-Sánchez ◽  
Mathilde J. H. Girard-Madoux ◽  
Aranzazu Cruz-Adalia
Keyword(s):  
Flow Cytometry ◽  
Innate Lymphoid Cells ◽  
Tissue Homeostasis ◽  
Innate Immune ◽  
Lymphoid Cells ◽  
Cell Marker ◽  
Single Cell Suspension ◽  
Optimized Protocol ◽  
Detailed Protocol

Since their discovery, innate lymphoid cells (ILCs) have gradually been gaining greater relevance in the field of immunology due to their multiple functions in the innate immune response. They can mainly be found in mucosal and barrier organs like skin, gut, and lungs, and have been classified into five main types (NKs, ILC1s, ILC2s, ILC3s, and Lti cells) according to their function and development. They all play major roles in functions such as tissue homeostasis, early pathogen defense, regulation of inflammation, or tissue remodeling. ILCs are mostly tissue-resident cells tightly bound to the tissue structure, a fact that requires long and complex protocols that do not always provide sufficient yield for analysis. This suggests the need for optimized approaches aimed at ensuring that enriched and viable ILC samples are obtained, in order to furnish quality results. Herein a detailed protocol is established for obtaining a single-cell suspension highly enriched in lymphoid cells from mouse gut in order to identify the different subsets of ILCs by means of flow cytometry. The cell marker panel and flow cytometry gating strategies for identification and quantification of all the different ILC populations are provided for simultaneous analysis. Moreover, the protocol described includes a procedure for studying the different cytokines produced by ILC3s involved in maintaining the integrity of the gut barrier and defending against extracellular pathogens. As a result, herein an efficient method is presented for studying mouse ILCs within the lamina propria of the small intestine and colon; this can constitute a useful tool for future investigations in the field.

Pandemic influenza virus, pH1N1, induces asthmatic symptoms via activation of innate lymphoid cells

2015 ◽  
Vol 26 (8) ◽  
pp. 780-788 ◽  
Author(s):  
D. H. Shim ◽  
Y. A. Park ◽  
M. J. Kim ◽  
J. Y. Hong ◽  
J. Y. Baek ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Pandemic Influenza ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells
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