Polygenic control of Caenorhabditis elegans fat storage

2006 ◽  
Vol 38 (3) ◽  
pp. 363-368 ◽  
Author(s):  
Ho Yi Mak ◽  
Laura S Nelson ◽  
Michael Basson ◽  
Carl D Johnson ◽  
Gary Ruvkun
Keyword(s):  
Caenorhabditis Elegans ◽  
Fat Storage ◽  
Polygenic Control

scholarly journals Regulation of Fat Storage and Reproduction by Krüppel-Like Transcription Factor KLF3 and Fat-Associated Genes in Caenorhabditis elegans

2011 ◽  
Vol 411 (3) ◽  
pp. 537-553 ◽  
Author(s):  
Jun Zhang ◽  
Razan Bakheet ◽  
Ranjit S. Parhar ◽  
Cheng-Han Huang ◽  
M. Mahmood Hussain ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Caenorhabditis Elegans ◽  
Fat Storage

scholarly journals A conserved family of proteins facilitates nascent lipid droplet budding from the ER

2015 ◽  
Vol 211 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Vineet Choudhary ◽  
Namrata Ojha ◽  
Andy Golden ◽  
William A. Prinz
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Lipid Metabolism ◽  
Caenorhabditis Elegans ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
De Novo ◽  
Fat Storage ◽  
Higher Eukaryotes ◽  
Er Membrane

Lipid droplets (LDs) are found in all cells and play critical roles in lipid metabolism. De novo LD biogenesis occurs in the endoplasmic reticulum (ER) but is not well understood. We imaged early stages of LD biogenesis using electron microscopy and found that nascent LDs form lens-like structures that are in the ER membrane, raising the question of how these nascent LDs bud from the ER as they grow. We found that a conserved family of proteins, fat storage-inducing transmembrane (FIT) proteins, is required for proper budding of LDs from the ER. Elimination or reduction of FIT proteins in yeast and higher eukaryotes causes LDs to remain in the ER membrane. Deletion of the single FIT protein in Caenorhabditis elegans is lethal, suggesting that LD budding is an essential process in this organism. Our findings indicated that FIT proteins are necessary to promote budding of nascent LDs from the ER.

scholarly journals Method development for investigating probiotic effects on fat storage in a Caenorhabditis elegans model (829.33)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Mack Shay ◽  
Marwa Al‐Tameemi ◽  
Saxon Wright ◽  
Robert McLean ◽  
Dhiraj Vattem ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Method Development ◽  
Fat Storage

scholarly journals The Role of Nuclear Receptor NHR-64 in Fat Storage Regulation in Caenorhabditis elegans

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9869 ◽  
Author(s):  
Bin Liang ◽  
Kim Ferguson ◽  
Lisa Kadyk ◽  
Jennifer L. Watts
Keyword(s):  
Caenorhabditis Elegans ◽  
Nuclear Receptor ◽  
Fat Storage

scholarly journals Rictor/TORC2 Regulates Caenorhabditis elegans Fat Storage, Body Size, and Development through sgk-1

PLoS Biology ◽  
2009 ◽  
Vol 7 (3) ◽  
pp. e1000060 ◽  
Author(s):  
Kevin T Jones ◽  
Elisabeth R Greer ◽  
David Pearce ◽  
Kaveh Ashrafi
Keyword(s):  
Body Size ◽  
Caenorhabditis Elegans ◽  
Fat Storage ◽  
Storage Body

scholarly journals Escherichia coli Metabolite Profiling Leads to the Development of an RNA Interference Strain for Caenorhabditis elegans

2019 ◽  
Vol 10 (1) ◽  
pp. 189-198 ◽  
Author(s):  
Isaiah A. A. Neve ◽  
Jessica N. Sowa ◽  
Chih-Chun J. Lin ◽  
Priya Sivaramakrishnan ◽  
Christophe Herman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Mitochondrial Morphology ◽  
Bacterial Strains ◽  
Hybrid Strain ◽  
Fat Storage ◽  
C Elegans ◽  
Interference Response ◽  
K 12

The relationship of genotypes to phenotypes can be modified by environmental inputs. Such crucial environmental inputs include metabolic cues derived from microbes living together with animals. Thus, the analysis of genetic effects on animals’ physiology can be confounded by variations in the metabolic profile of microbes. Caenorhabditis elegans exposed to distinct bacterial strains and species exhibit phenotypes different at cellular, developmental, and behavioral levels. Here we reported metabolomic profiles of three Escherichia coli strains, B strain OP50, K-12 strain MG1655, and B-K-12 hybrid strain HB101, as well as different mitochondrial and fat storage phenotypes of C. elegans exposed to MG1655 and HB101 vs. OP50. We found that these metabolic phenotypes of C. elegans are not correlated with overall metabolic patterning of bacterial strains, but their specific metabolites. In particular, the fat storage phenotype is traced to the betaine level in different bacterial strains. HT115 is another K-12 E. coli strain that is commonly utilized to elicit an RNA interference response, and we showed that C. elegans exposed to OP50 and HT115 exhibit differences in mitochondrial morphology and fat storage levels. We thus generated an RNA interference competent OP50 (iOP50) strain that can robustly and consistently knockdown endogenous C. elegans genes in different tissues. Together, these studies suggest the importance of specific bacterial metabolites in regulating the host’s physiology and provide a tool to prevent confounding effects when analyzing genotype-phenotype interactions under different bacterial backgrounds.

scholarly journals A Comparative Study of Fat Storage Quantitation in Nematode Caenorhabditis elegans Using Label and Label-Free Methods

PLoS ONE ◽  
2010 ◽  
Vol 5 (9) ◽  
pp. e12810 ◽  
Author(s):  
Kelvin Yen ◽  
Thuc T. Le ◽  
Ankita Bansal ◽  
Sri Devi Narasimhan ◽  
Ji-Xin Cheng ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Comparative Study ◽  
Label Free ◽  
Nematode Caenorhabditis Elegans ◽  
Fat Storage

scholarly journals Escherichia coli Metabolite Profiling Leads to the Development of an RNA Interference Strain for Caenorhabditis elegans

2019 ◽  
Author(s):  
Isaiah A. A. Neve ◽  
Jessica N. Sowa ◽  
Chih-Chun J. Lin ◽  
Priya Sivaramakrishnan ◽  
Christophe Herman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Mitochondrial Morphology ◽  
Bacterial Strains ◽  
Hybrid Strain ◽  
Fat Storage ◽  
C Elegans ◽  
Interference Response ◽  
K 12

AbstractThe relationship of genotypes to phenotypes can be modified by environmental inputs. Such crucial environmental inputs include metabolic cues derived from microbes living together with animals. Thus the analysis of genetic effects on animals’ physiology can be confounded by variations in the metabolic profile of microbes. Caenorhabditis elegans exposed to distinct bacterial strains and species exhibit phenotypes different at cellular, developmental and behavioral levels. Here we reported metabolomic profiles of three Escherichia coli strains, B strain OP50, K-12 strain MG1655, and B-K-12 hybrid strain HB101, and also different mitochondrial and fat storage phenotypes of C. elegans exposed to MG1655 and HB101 versus OP50. We found that these metabolic phenotypes of C. elegans are not correlated with overall metabolic patterning of bacterial strains, but their specific metabolites. In particular, the fat storage phenotype is traced to the betaine level in different bacterial strains. HT115 is another K-12 E. coli strain that is commonly utilized to elicit an RNA interference response, and we showed that C. elegans exposed to OP50 and HT115 exhibit differences in mitochondrial morphology and fat storage levels. We thus generated an RNA interference competent OP50 (iOP50) strain that can robustly and consistently knockdown endogenous C. elegans genes in different tissues. Together, these studies suggest the importance of specific bacterial metabolites in regulating the host’s physiology, and provide a tool to prevent confounding effects when analyzing genotype-phenotype interactions under different bacterial backgrounds.

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