scholarly journals Correction: Corrigendum: Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome

1993 ◽  
Vol 4 (3) ◽  
pp. 320-320
Keyword(s):  
Transgenic Mice ◽  
Heavy Chain ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Immunoglobulin Gene ◽  
Gene Fragment

Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome

1993 ◽  
Vol 4 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Ted K. Choi ◽  
Paul W. Hollenbach ◽  
Barbara E. Pearson ◽  
Roanna M. Ueda ◽  
Gregory N. Weddell ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Heavy Chain ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Immunoglobulin Gene ◽  
Gene Fragment

A yeast artificial chromosome covering the tyrosinase gene confers copy number-dependent expression in transgenic mice

Nature ◽  
1993 ◽  
Vol 362 (6417) ◽  
pp. 258-261 ◽  
Author(s):  
Andreas Schedl ◽  
Lluís Montoliu ◽  
Gavin Kelsey ◽  
Günther Schütz
Keyword(s):  
Transgenic Mice ◽  
Copy Number ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Tyrosinase Gene

Altered repertoire of endogenous immunoglobulin gene expression in transgenic mice containing a rearranged Mu heavy chain gene

Cell ◽  
1986 ◽  
Vol 45 (2) ◽  
pp. 247-259 ◽  
Author(s):  
David Weaver ◽  
Moema H. Reis ◽  
Christopher Albanese ◽  
Frank Costantini ◽  
David Baltimore ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Heavy Chain ◽  
Chain Gene ◽  
Immunoglobulin Gene ◽  
Heavy Chain Gene

scholarly journals Germ-line transmission and developmental regulation of a 150-kb yeast artificial chromosome containing the human beta-globin locus in transgenic mice

1993 ◽  
Vol 90 (23) ◽  
pp. 11381-11385 ◽  
Author(s):  
K M Gaensler ◽  
M Kitamura ◽  
Y W Kan
Keyword(s):  
Transgenic Mice ◽  
Yeast Artificial Chromosome ◽  
Germ Line ◽  
Physiological Role ◽  
Artificial Chromosome ◽  
Chromatin Domain ◽  
Globin Genes ◽  
Beta Globin ◽  
Cis Acting ◽  
Artificial Chromosomes

Sequential expression of the genes of the human beta-globin locus requires the formation of an erythroid-specific chromatin domain spanning > 200 kb. Regulation of this gene family involves both local interactions with proximal cis-acting sequences and long-range interactions with control elements upstream of the locus. To make it possible to analyze the interactions of cis-acting sequences of the human beta-globin locus in their normal spatial and sequence context, we characterized two yeast artificial chromosomes (YACs) 150 and 230 kb in size, containing the entire beta-globin locus. We have now successfully integrated the 150-kb YAC into the germ line of transgenic mice as a single unrearranged fragment that includes the locus control region, structural genes, and 30 kb of 3' flanking sequences present in the native locus. Expression of the transgenic human beta-globin locus is tissue- and developmental stage-specific and closely follows the pattern of expression of the endogenous mouse beta-globin locus. By using homology-directed recombination in yeast and methods for the purification and transfer of YACs into transgenic mice, it will now be feasible to study the physiological role of cis-acting sequences in specifying an erythroid-specific chromatin domain and directing expression of beta-globin genes during ontogeny.

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