Evolutionary sequence comparisons using high-density oligonucleotide arrays

1998 ◽  
Vol 18 (2) ◽  
pp. 155-158 ◽  
Author(s):  
Joseph G. Hacia ◽  
Wojciech Makalowski ◽  
Keith Edgemon ◽  
Michael R. Erdos ◽  
Christiane M. Robbins ◽  
...  
Keyword(s):  
High Density ◽  
Evolutionary Sequence ◽  
Sequence Comparisons ◽  
Oligonucleotide Arrays

scholarly journals Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists

1996 ◽  
Vol 93 (24) ◽  
pp. 13555-13560 ◽  
Author(s):  
G. McGall ◽  
J. Labadie ◽  
P. Brock ◽  
G. Wallraff ◽  
T. Nguyen ◽  
...  
Keyword(s):  
High Density ◽  
Oligonucleotide Arrays ◽  
Directed Synthesis

[29] Use of laser capture microdissection-generated targets for hybridization of high-density oligonucleotide arrays

2002 ◽  
pp. 323-333 ◽  
Author(s):  
Hiroe Ohyama ◽  
Mamatha Mahadevappa ◽  
Heikki Luukkaa ◽  
Randy Todd ◽  
Janet A Warrington ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
High Density ◽  
Oligonucleotide Arrays ◽  
Laser Capture

scholarly journals Comparing the global mRNA expression profile of human atrial and ventricular myocardium with high-density oligonucleotide arrays

2005 ◽  
Vol 129 (6) ◽  
pp. 1383-1390 ◽  
Author(s):  
Peter Ellinghaus ◽  
Robert J. Scheubel ◽  
Dobromir Dobrev ◽  
Ursula Ravens ◽  
Juergen Holtz ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Expression Profile ◽  
Ventricular Myocardium ◽  
High Density ◽  
Mrna Expression Profile ◽  
Oligonucleotide Arrays

Gene expression in PBMC from severe atopic dermatitis patients assessed by high-density oligonucleotide arrays

2002 ◽  
Vol 109 (1) ◽  
pp. S111-S111
Author(s):  
Masayuki Heishi ◽  
Shinji Kagaya ◽  
T Katsunuma ◽  
Akira Akasawa ◽  
Akiko Nakada ◽  
...  
Keyword(s):  
Gene Expression ◽  
Atopic Dermatitis ◽  
High Density ◽  
Severe Atopic Dermatitis ◽  
Oligonucleotide Arrays

scholarly journals Probe selection for high-density oligonucleotide arrays

2003 ◽  
Vol 100 (20) ◽  
pp. 11237-11242 ◽  
Author(s):  
R. Mei ◽  
E. Hubbell ◽  
S. Bekiranov ◽  
M. Mittmann ◽  
F. C. Christians ◽  
...  
Keyword(s):  
High Density ◽  
Oligonucleotide Arrays ◽  
Probe Selection ◽  
Selection For

Assessing DNA Sequence Variations in Human ESTs in a Phylogenetic Context Using High-Density Oligonucleotide Arrays

Genomics ◽  
2002 ◽  
Vol 80 (3) ◽  
pp. 351-360 ◽  
Author(s):  
Jian-Bing Fan ◽  
Dominik Gehl ◽  
Linda Hsie ◽  
Naiping Shen ◽  
Kerstin Lindblad-Toh ◽  
...  
Keyword(s):  
Dna Sequence ◽  
High Density ◽  
Oligonucleotide Arrays ◽  
Sequence Variations ◽  
Human Ests

scholarly journals Parallel Gene Expression Monitoring using Oligonucleotide Probe Arrays of Multiple Transcripts with an Animal Model of Focal Ischemia

2000 ◽  
Vol 20 (7) ◽  
pp. 1045-1055 ◽  
Author(s):  
Marc A. Soriano ◽  
Michel Tessier ◽  
Ulrich Certa ◽  
Ramanjit Gill
Keyword(s):  
Time Course ◽  
Oligonucleotide Probe ◽  
Neuronal Degeneration ◽  
Focal Ischemia ◽  
Brain Regions ◽  
Artery Occlusion ◽  
High Density ◽  
Oligonucleotide Arrays ◽  
Multiple Transcripts ◽  
First Case

High density oligonucleotide arrays offer tremendous potential to study gene changes occurring in disease states. The authors described the first case of using a custom designed high density oligonucleotide probe array containing 750 genes to monitor the changes in mRNA transcript levels occurring after focal ischemia for a period of 3 hours. Permanent middle cerebral artery occlusion in the rat resulted in neuronal degeneration in the dorsolateral cortex and striatum over a time course of 24 hours. Comparing the changes in hybridization levels in the frontal and parietal cortices and the striatum, between the ipsilateral and contralateral sides of the brain using the probe arrays resulted in the up-regulation of 24 genes, which showed greater than a twofold change. Very few genes were found to be downregulated after the ischemic insult. Many of the immediate early genes (IEGs) such as c-fos, NGFI-A, NGFI-C, and Krox-20 were found to be robustly up-regulated in the three different regions studied. Other genes that were up-regulated in perifocal regions included Arc, Inhibin-β-A, and the phosphatases MKP-1 and MKP-3. The hybridization signal intensity from the probe arrays enabled quantification of many genes relative to one another, and robust changes in expression were obtained with very little interanimal variability. Furthermore, the authors were able to validate the increased expression of NGFI-C and Arc using in situ hybridization. This represented the first example of using high density oligonucleotide probe arrays in studying the expression of many genes in parallel and in discrete brain regions after focal ischemia.

Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysis

1996 ◽  
Vol 14 (4) ◽  
pp. 441-447 ◽  
Author(s):  
Joseph G. Hacia ◽  
Lawrence C. Brody ◽  
Mark S. Chee ◽  
Stephen P.A. Fodor ◽  
Francis S. Collins
Keyword(s):  
Fluorescence Analysis ◽  
High Density ◽  
Oligonucleotide Arrays
Sign in / Sign up

Export Citation Format

Share Document