Towards gene therapy for haemophilia B using primary human keratinocytes

1993 ◽  
Vol 3 (2) ◽  
pp. 180-183 ◽  
Author(s):  
Ann J. Gerrard ◽  
David L. Hudson ◽  
George G. Brownlee ◽  
Fiona M. Watt
Keyword(s):  
Gene Therapy ◽  
Human Keratinocytes ◽  
Primary Human Keratinocytes ◽  
Haemophilia B

High-Level Secretion of Growth Hormone by Retrovirally Transduced Primary Human Keratinocytes: Prospects for an Animal Model of Cutaneous Gene Therapy

2006 ◽  
Vol 34 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Cibele Nunes Peroni ◽  
Cláudia Regina Cecchi ◽  
Renata Damiani ◽  
Carlos R. J. Soares ◽  
Maria Teresa C. P. Ribela ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Growth Hormone ◽  
Animal Model ◽  
Human Keratinocytes ◽  
Primary Human Keratinocytes ◽  
High Level

Secretion of mouse growth hormone by transduced primary human keratinocytes: prospects for an animal model of cutaneous gene therapy

2008 ◽  
Vol 10 (7) ◽  
pp. 734-743 ◽  
Author(s):  
Cibele Nunes Peroni ◽  
Cláudia Regina Cecchi ◽  
Cristiane Wanderley Rosauro ◽  
Suely Nonogaki ◽  
Enrique Boccardo ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Growth Hormone ◽  
Animal Model ◽  
Human Keratinocytes ◽  
Primary Human Keratinocytes

Expression profiling of vitamin D treated primary human keratinocytes

2007 ◽  
Vol 100 (3) ◽  
pp. 574-592 ◽  
Author(s):  
Pamela Renate Moll ◽  
Veronika Sander ◽  
Anna-Maria Frischauf ◽  
Klaus Richter
Keyword(s):  
Vitamin D ◽  
Expression Profiling ◽  
Human Keratinocytes ◽  
Primary Human Keratinocytes

scholarly journals HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Irma Colombo ◽  
Enrico Sangiovanni ◽  
Roberta Maggio ◽  
Carlo Mattozzi ◽  
Stefania Zava ◽  
...  
Keyword(s):  
Cell Density ◽  
Skin Diseases ◽  
Inflammatory Responses ◽  
Human Keratinocytes ◽  
Suitable Model ◽  
Hacat Cells ◽  
Primary Human Keratinocytes ◽  
Proinflammatory Mediators ◽  
Cytokines And Chemokines

Cultured primary human keratinocytes are frequently employed for studies of immunological and inflammatory responses; however, interpretation of experimental data may be complicated by donor to donor variability, the relatively short culture lifetime, and variations between passages. To standardize the in vitro studies on keratinocytes, we investigated the use of HaCaT cells, a long-lived, spontaneously immortalized human keratinocyte line which is able to differentiate in vitro, as a suitable model to follow the release of inflammatory and repair mediators in response to TNFα or IL-1β. Different treatment conditions (presence or absence of serum) and differentiation stimuli (increase in cell density as a function of time in culture and elevation of extracellular calcium) were considered. ELISA and Multiplex measurement technologies were used to monitor the production of cytokines and chemokines. Taken together, the results highlight that Ca2+ concentration in the medium, cell density, and presence of serum influences at different levels the release of proinflammatory mediators by HaCaT cells. Moreover, HaCaT cells maintained in low Ca2+ medium and 80% confluent are similar to normal keratinocytes in terms of cytokine production suggesting that HaCaT cells may be a useful model to investigate anti-inflammatory interventions/therapies on skin diseases.

Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mammalian wounds

1996 ◽  
Vol 109 (1) ◽  
pp. 199-207 ◽  
Author(s):  
K.Y. Nishimura ◽  
R.R. Isseroff ◽  
R. Nuccitelli
Keyword(s):  
Electric Fields ◽  
Human Keratinocytes ◽  
Voltage Gradient ◽  
Primary Human Keratinocytes ◽  
Directed Movement ◽  
Average Cell ◽  
Negative Pole ◽  
Mammalian Skin ◽  
Dc Electric Fields

Previous measurements of the lateral electric fields near skin wounds in guinea pigs have detected DC fields between 100–200 mV/mm near the edge of the wound. We have studied the translocation response of motile primary human keratinocytes migrating on a collagen substrate while exposed to similar physiological DC electric fields. We find that keratinocytes migrate randomly on collagen in fields of 5 mV/mm or less, but in larger fields they migrate towards the negative pole of the field, exhibiting galvanotaxis. Since these cells have an average cell length of 50 microns, this implies that they are able to detect a voltage gradient as low as 0.5 mV along their length. This cath-odally-directed movement exhibits increased directedness with increasing field strengths between 10 and 100 mV/mm. We observe a maximally directed response at 100 mV/mm with half of the cells responding to the field within 14 minutes. The average speed of migration tended to be greater in fields above 50 mV/mm than in smaller fields. We conclude that human keratinocytes migrate towards the negative pole in DC electric fields that are of the same magnitude as measured in vivo near wounds in mammalian skin.

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