scholarly journals In vivo growth of a mouse prostate gland from a single stem cell

2009 ◽  
Vol 6 (1) ◽  
pp. 3-3
Keyword(s):  
Stem Cell ◽  
Prostate Gland ◽  
Mouse Prostate ◽  
In Vivo Growth

scholarly journals Estrogen receptor 1 expression and methylation of Esr1 promoter in mouse fetal prostate mesenchymal cells induced by gestational exposure to bisphenol A or ethinylestradiol

2019 ◽  
Vol 5 (3) ◽  
Author(s):  
Ramji K Bhandari ◽  
Julia A Taylor ◽  
Jennifer Sommerfeld-Sager ◽  
Donald E Tillitt ◽  
William A Ricke ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bisphenol A ◽  
Oral Contraceptives ◽  
Primary Cultures ◽  
Prostate Gland ◽  
Mesenchymal Cells ◽  
Urogenital Sinus ◽  
Environmental Estrogen ◽  
Mouse Prostate

Abstract Fetal/neonatal environmental estrogen exposures alter developmental programing of the prostate gland causing onset of diseases later in life. We have previously shown in vitro that exposures to 17β-estradiol (E2) and the endocrine disrupting chemical bisphenol A, at concentrations relevant to human exposure, cause an elevation of estrogen receptor α (Esr1) mRNA in primary cultures of fetal mouse prostate mesenchymal cells; a similar result was observed in the fetal rat urogenital sinus. Effects of these chemicals on prostate mesenchyme in vivo are not well understood. Here we show effects in mice of fetal exposure to the estrogenic drug in mixed oral contraceptives, 17α-ethinylestradiol (EE2), at a concentration of EE2 encountered by human embryos/fetuses whose mothers become pregnant while on EE2-containing oral contraceptives, or bisphenol A at a concentration relevant to exposures observed in human fetuses in vivo. Expression of Esr1 was elevated by bisphenol A or EE2 exposures, which decreased the global expression of DNA methyltransferase 3A (Dnmt3a), while methylation of Esr1 promoter was significantly increased. These results show that exposures to the environmental estrogen bisphenol A and drug EE2 cause transcriptional and epigenetic alterations to expression of estrogen receptors in developing prostate mesenchyme in vivo.

scholarly journals New Chondrosarcoma Cell Lines with Preserved Stem Cell Properties to Study the Genomic Drift During In Vitro/In Vivo Growth

2019 ◽  
Vol 8 (4) ◽  
pp. 455 ◽  
Author(s):  
Veronica Rey ◽  
Sofia T. Menendez ◽  
Oscar Estupiñan ◽  
Aida Rodriguez ◽  
Laura Santos ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cell Lines ◽  
Isocitrate Dehydrogenase ◽  
Cancer Genomics ◽  
Sequencing Analysis ◽  
Isocitrate Dehydrogenase 2 ◽  
In Vivo Growth

For the cancer genomics era, there is a need for clinically annotated close-to-patient cell lines suitable to investigate altered pathways and serve as high-throughput drug-screening platforms. This is particularly important for drug-resistant tumors like chondrosarcoma which has few models available. Here we established and characterized new cell lines derived from two secondary (CDS06 and CDS11) and one dedifferentiated (CDS-17) chondrosarcomas as well as another line derived from a CDS-17-generated xenograft (T-CDS17). These lines displayed cancer stem cell-related and invasive features and were able to initiate subcutaneous and/or orthotopic animal models. Different mutations in Isocitrate Dehydrogenase-1 (IDH1), Isocitrate Dehydrogenase-2 (IDH2), and Tumor Supressor P53 (TP53) and deletion of Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) were detected both in cell lines and tumor samples. In addition, other mutations in TP53 and the amplification of Mouse Double Minute 2 homolog (MDM2) arose during cell culture in CDS17 cells. Whole exome sequencing analysis of CDS17, T-CDS17, and matched patient samples confirmed that cell lines kept the most relevant mutations of the tumor, uncovered new mutations and revealed structural variants that emerged during in vitro/in vivo growth. Altogether, this work expanded the panel of clinically and genetically-annotated chondrosarcoma lines amenable for in vivo studies and cancer stem cell (CSC) characterization. Moreover, it provided clues of the genetic drift of chondrosarcoma cells during the adaptation to grow conditions.

Monitoring stem cell migration in the nervous system by in vivo magnetic resonance imaging

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S692-S692
Author(s):  
Mathias Hoehn ◽  
Uwe Himmelreich ◽  
Ralph Weber ◽  
Pedro Ramos-Cabrer ◽  
Susanne Wegener ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Nervous System ◽  
Stem Cell ◽  
Cell Migration ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Stem Cell Migration
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