scholarly journals Single-cell analysis and stochastic modelling unveil large cell-to-cell variability in influenza A virus infection

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Frank S. Heldt ◽  
Sascha Y. Kupke ◽  
Sebastian Dorl ◽  
Udo Reichl ◽  
Timo Frensing
Keyword(s):  
Virus Infection ◽  
Single Cell ◽  
Influenza A Virus ◽  
Influenza A ◽  
Single Cell Analysis ◽  
Stochastic Modelling ◽  
Large Cell ◽  
Cell Analysis ◽  
Influenza A Virus Infection ◽  
Cell To Cell Variability

scholarly journals Single-Cell Analysis Uncovers a Vast Diversity in Intracellular Viral Defective Interfering RNA Content Affecting the Large Cell-to-Cell Heterogeneity in Influenza A Virus Replication

Viruses ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 71 ◽  
Author(s):  
Sascha Young Kupke ◽  
Lam-Ha Ly ◽  
Stefan Thomas Börno ◽  
Alexander Ruff ◽  
Bernd Timmermann ◽  
...  
Keyword(s):  
Single Cell ◽  
Influenza A Virus ◽  
Virus Replication ◽  
Influenza A ◽  
Single Cell Analysis ◽  
Virus Production ◽  
Large Cell ◽  
Cell Heterogeneity ◽  
Viral Mrnas ◽  
Cell To Cell Variability

Virus replication displays a large cell-to-cell heterogeneity; yet, not all sources of this variability are known. Here, we study the effect of defective interfering (DI) particle (DIP) co-infection on cell-to-cell variability in influenza A virus (IAV) replication. DIPs contain a large internal deletion in one of their eight viral RNAs (vRNA) and are, thus, defective in virus replication. Moreover, they interfere with virus replication. Using single-cell isolation and reverse transcription polymerase chain reaction, we uncovered a large between-cell heterogeneity in the DI vRNA content of infected cells, which was confirmed for DI mRNAs by single-cell RNA sequencing. A high load of intracellular DI vRNAs and DI mRNAs was found in low-productive cells, indicating their contribution to the large cell-to-cell variability in virus release. Furthermore, we show that the magnitude of host cell mRNA expression (some factors may inhibit virus replication), but not the ribosome content, may further affect the strength of single-cell virus replication. Finally, we show that the load of viral mRNAs (facilitating viral protein production) and the DI mRNA content are, independently from one another, connected with single-cell virus production. Together, these insights advance single-cell virology research toward the elucidation of the complex multi-parametric origin of the large cell-to-cell heterogeneity in virus infections.

scholarly journals Induction of cyclophilin A by influenza A virus infection facilitates group A Streptococcus coinfection

Cell Reports ◽  
2021 ◽  
Vol 35 (7) ◽  
pp. 109159
Author(s):  
Xiaoyuan Bai ◽  
Wenxian Yang ◽  
Xiaohan Luan ◽  
Huizi Li ◽  
Heqiao Li ◽  
...  
Keyword(s):  
Virus Infection ◽  
Influenza A Virus ◽  
Influenza A ◽  
Group A Streptococcus ◽  
Cyclophilin A ◽  
Group A ◽  
Influenza A Virus Infection

scholarly journals Dicer is involved in protection against influenza A virus infection

2007 ◽  
Vol 88 (10) ◽  
pp. 2627-2635 ◽  
Author(s):  
Alexey A. Matskevich ◽  
Karin Moelling
Keyword(s):  
Virus Infection ◽  
Influenza A Virus ◽  
Mammalian Cells ◽  
Influenza A ◽  
Virus Production ◽  
Vero Cells ◽  
Antiviral Response ◽  
Protein Levels ◽  
Infected Cells ◽  
Influenza A Virus Infection

In mammals the interferon (IFN) system is a central innate antiviral defence mechanism, while the involvement of RNA interference (RNAi) in antiviral response against RNA viruses is uncertain. Here, we tested whether RNAi is involved in the antiviral response in mammalian cells. To investigate the role of RNAi in influenza A virus-infected cells in the absence of IFN, we used Vero cells that lack IFN-α and IFN-β genes. Our results demonstrate that knockdown of a key RNAi component, Dicer, led to a modest increase of virus production and accelerated apoptosis of influenza A virus-infected cells. These effects were much weaker in the presence of IFN. The results also show that in both Vero cells and the IFN-producing alveolar epithelial A549 cell line influenza A virus targets Dicer at mRNA and protein levels. Thus, RNAi is involved in antiviral response, and Dicer is important for protection against influenza A virus infection.

scholarly journals A Functional Variation in CD55 Increases the Severity of 2009 Pandemic H1N1 Influenza A Virus Infection

2012 ◽  
Vol 206 (4) ◽  
pp. 495-503 ◽  
Author(s):  
Jie Zhou ◽  
Kelvin Kai-Wang To ◽  
Hui Dong ◽  
Zhong-Shan Cheng ◽  
Candy Choi-Yi Lau ◽  
...  
Keyword(s):  
Virus Infection ◽  
Influenza A Virus ◽  
Influenza A ◽  
H1n1 Influenza ◽  
Pandemic H1n1 ◽  
Functional Variation ◽  
Pandemic H1n1 Influenza ◽  
Influenza A Virus Infection

scholarly journals Dendritic cells and influenza A virus infection

Virulence ◽  
2012 ◽  
Vol 3 (7) ◽  
pp. 603-608 ◽  
Author(s):  
Jason Waithman ◽  
Justine D. Mintern
Keyword(s):  
Dendritic Cells ◽  
Virus Infection ◽  
Influenza A Virus ◽  
Influenza A ◽  
Influenza A Virus Infection
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