scholarly journals Quadruple-junction lattice coherency and phase separation in a binary-phase system

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Sung-Yoon Chung ◽  
Si-Young Choi ◽  
Jin-Gyu Kim ◽  
Young-Min Kim
Keyword(s):  
Phase Separation ◽  
Phase System ◽  
Binary Phase

Transmission Electron Microscopy (TEM) Observations of Phase-Separations of Gamma-Prime Precipitates in Ni-Al-Fe and Ni-Si-Fe Ternary Alloys

2007 ◽  
Vol 26-28 ◽  
pp. 1311-1314 ◽  
Author(s):  
M. Senga ◽  
H. Kumagai ◽  
Tomokazu Moritani ◽  
Minoru Doi
Keyword(s):  
Phase Separation ◽  
Volume Fraction ◽  
Heating Temperature ◽  
Ternary Alloys ◽  
Phase System ◽  
Precipitate Phase ◽  
Two Phase ◽  
Gamma Prime ◽  
Transmission Electron ◽  
The Difference

In Ni-13.0at%Si-3.1at%Fe alloy, when γ/γ’ two-phase microstructure formed at 1123 K is isothermally heated at 923 K which is lower than the temperature where the initial γ/γ’ microstructure forms, the phase-separation of γ/γ’ precipitate phase occurs and γ particles newly appear in each cuboidal γ’ precipitate. While in Ni-10.2at%Al-10.8at%Fe alloy, when γ/γ’ two-phase microstructure formed at 1023 K is isothermally heated at 1123 K which is higher than the temperature where the initial γ/γ’ microstructure forms, the phase-separation of γ’ precipitate phase takes place and γ particles newly appear in each cuboidal γ’ precipitate. Such appearance of new γ particles in γ’ precipitates can be explained by the difference in the volume fraction of γ phase that should exist in the γ/γ’ two-phase system depending on the heating temperature.

Self assembly of cyclic polygon shaped fluid colloidal membranes through pinning

Soft Matter ◽  
2018 ◽  
Vol 14 (48) ◽  
pp. 9959-9966
Author(s):  
Lachit Saikia ◽  
Prerna Sharma
Keyword(s):  
Phase Separation ◽  
Self Assembly ◽  
Anisotropic Fluid ◽  
Binary Phase ◽  
Colloidal Membranes ◽  
Cyclic Polygon ◽  
Colloidal Rods

Binary phase separation of geometrically different colloidal rods in the presence of pinning leads to cyclic polygon shaped anisotropic fluid colloidal membranes.

Producing PVP Nanofibers by Electrospinning in N2

2012 ◽  
Vol 560-561 ◽  
pp. 701-708 ◽  
Author(s):  
Lu Li ◽  
Jie Xu ◽  
Tao Fang ◽  
Jin Geng ◽  
Detlef Freitag ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Phase Equilibrium ◽  
Phase Separation ◽  
Phase Behavior ◽  
Polymer Solution ◽  
Aspen Plus ◽  
Good Choice ◽  
The Other ◽  
Binary Phase ◽  
Novel Method

Electrospinning combined with nonsolvent-induced phase separation is a simple and novel method to produce porous nanofibers. In the study, Poly (vinylpyrrolidone) (PVP) nanofibers were fabricated using an electrospinning approach complemented by compressed nitrogen (N). N2 was used as the nonsolvent of choice. Besides, the tun2ning of N2 pressure and temperature can impact the nanofibers’ morphologies by altering phase behavior and mass transfer. Also, the other parameters affecting electrospinning of polymer solution were discussed. The results were demonstrated by extending the technique to PVP/dichloromethane (DCM) and PVP/ethanol (EtOH) systems. And the binary phase equilibrium of solvents (dichloromethane or ethanol) and N simulated by ASPEN PLUS 2006 demonstrates that N is not a 2good choice for producing hollow or po2rous polymer nanofibers.

Upflow Anaerobic Sludge Digestion in a Phase Separation System

1993 ◽  
Vol 28 (7) ◽  
pp. 133-138 ◽  
Author(s):  
Hsin-Yi Lin ◽  
Chaio-Fuei Ouyang
Keyword(s):  
Phase Separation ◽  
Gas Production ◽  
Anaerobic Sludge ◽  
Phase System ◽  
Volatile Acid ◽  
Two Phase ◽  
Cell Biomass ◽  
Sludge Digestion ◽  
Methane Production Rate ◽  
The One

This paper aims to expand the knowledge about methods for improving the rate of solids hydrolysis, volatile acid formation and methane gas production. It studies the effects of phase separation and operational modes on the performance of the process. Two systems were investigated. The two-phase system consisted of a CSTR acid-phase digester and an upflow methane-phase digester, the one-phase system comprised of an upflow anaerobic digester. In order to provide optimal contact conditions and recycle liberated indigenous enzymes or cell biomass fractions, continuous recycling of the effluent sludges to the sludge bed of the reactor was used. In general, the nonbiodegradable portion of the substrates contained in the mixed sludges was found to be about 45% on the basis of TVS. The TVS reduction of the two-phase system was 53%, this is higher than in the one-phase system. Methane production rate and methane content were 0.351 l/g TVS added and 66.6%, respectively.

scholarly journals Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

2021 ◽  
Author(s):  
Sohyung Lee ◽  
Joe de Rutte ◽  
Robert Dimatteo ◽  
Doyeon Koo ◽  
Dino Di Carlo
Keyword(s):  
Phase Separation ◽  
Phase System ◽  
Cell Secretion ◽  
Two Phase ◽  
Hollow Shell ◽  
Hydrogel Microparticles ◽  
Trade Offs ◽  
Shell Particles ◽  
Scalable Production ◽  
Formation Phase

Microparticles with defined shapes and spatial chemical modification can enable new opportunities to interface with cells and tissues at the cellular scale. However, conventional methods to fabricate shaped microparticles have trade-offs between the throughput of manufacture and precision of particle shape and chemical functionalization. Here, we achieved scalable production of hydrogel microparticles at rates of greater than 40 million/hour with localized surface chemistry using a parallelized step emulsification device and temperature-induced phase-separation. The approach harnesses a polymerizable polyethylene glycol (PEG) and gelatin aqueous-two phase system (ATPS) which conditionally phase separates within microfluidically-generated droplets. Following droplet formation, phase separation is induced and phase separated droplets are subsequently crosslinked to form uniform crescent and hollow shell particles with gelatin functionalization on the boundary of the cavity. The gelatin localization enabled deterministic cell loading in nanoliter-sized crescent-shaped particles, which we refer to as nanovials, with cavity dimensions tuned to the size of cells. Loading on nanovials also imparted improved cell viability during analysis and sorting using standard fluorescence activated cell sorters, presumably by protecting cells from shear stress. This localization effect was further exploited to selectively functionalize capture antibodies to nanovial cavities enabling single-cell secretion assays with reduced cross-talk in a simplified format.

scholarly journals Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems

Antibodies ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 40 ◽  
Author(s):  
Thomas Kruse ◽  
Axel Schmidt ◽  
Markus Kampmann ◽  
Jochen Strube
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Chinese Hamster ◽  
Contact Angles ◽  
Cell Protein ◽  
Phase System ◽  
Separation Performance ◽  
Downstream Process ◽  
Two Phase ◽  
Cultivation Broth

Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An efficient purification of the mAb was accomplished by the ATPS composition. The phase separation was realized by a newly developed membrane based phase separator. Moreover, a complete cell removal was integrated into this process by the used membrane. A selectivity between both phases was achieved by membrane modification. Yields up to 93% in the light phase and removal of process related impurities were obtained after aqueous two-phase extraction (ATPE). Phase separation performance as well as contact angles on the membrane were characterized for different ATPS. ATPE directly from the cultivation broth in combination with the new membrane based phase separation led to a mAb yield of 78% with a simultaneous reduction of deoxyribonucleic acid (DNA) and host cell protein (HCP) load.

Electron Microscopy Studies of Polymer Blends

1980 ◽  
Vol 38 ◽  
pp. 248-249
Author(s):  
S.Y. Hobbs ◽  
V.H. Watkins ◽  
R.P. Kambour ◽  
R.C. Bopp
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Separation ◽  
Phase System ◽  
Segregation Behavior ◽  
Phenylene Oxide ◽  
Transmission Electron ◽  
Microscopic Studies ◽  
Phase Separation Behavior ◽  
Separation Behavior

Scanning and transmission electron microscopy have been used to investigate the phase separation behavior of blends of brominated poly (2,6-dimethy1 -1 ,4-phenylene oxide) known trivially as poly (xylenyl ether) (PXE) and polystyrene. DSC scans show that when more than 85% of the PXE rings are brominated, the blends exhibit two glass temperatures centered at 100°C and 290°C and the polymers are immiscible.1 At lower bromine concentrations, a single composition dependent Tg characteristic of a one phase system is observed. Microscopic studies, however, indicate that separate phases which can not be detected ca1orimetrica11y may persist to considerably lower levels of bromination and may thus provide a more sensitive measure of segregation behavior.

Phase-Separation of B2 Precipitates in an Fe-Ni-Al Alloy

2010 ◽  
Vol 638-642 ◽  
pp. 2274-2278 ◽  
Author(s):  
Yasuhiro Kuno ◽  
Yasuo Nakane ◽  
Takao Kozakai ◽  
Minoru Doi ◽  
Junji Yamanaka ◽  
...  
Keyword(s):  
Free Energy ◽  
Phase Separation ◽  
Volume Fraction ◽  
Heating Temperature ◽  
Phase System ◽  
Al Alloy ◽  
Two Phase ◽  
Chemical Free Energy ◽  
The Difference ◽  
The Individual

When Fe-10.3mol%Ni-14.3mol%Al alloy is heated at 1173 K for 8.64104 s, a number of B2 precipitates are dispersed in the A2 matrix. When the two-phase microstructure of A2+B2 is aged at 973 K, the phase-separation of B2 precipitate particles takes place to form a new A2 phase in each B2 particle. In the course of further ageing at 973 K, the new A2 phase grows but decreases in number, and finally only one A2 particle is left in the individual B2 particles. The appearance of new A2 phase in each B2 precipitate is due to the difference in the volume fraction of A2 phase that should exist in A2+B2 two-phase system depending on the heating temperature: i.e., the phase-separation of B2 precipitates starts with the aid of chemical free energy.

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