scholarly journals A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Joon-Yung Cha ◽  
Woe-Yeon Kim ◽  
Sun Bin Kang ◽  
Jeong Im Kim ◽  
Dongwon Baek ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Reductase Activity ◽  
Auxin Biosynthesis ◽  
Thiol Reductase

scholarly journals REDOX Reaction at ASK1-Cys250 Is Essential for Activation of JNK and Induction of Apoptosis

2009 ◽  
Vol 20 (16) ◽  
pp. 3628-3637 ◽  
Author(s):  
Philippe J. Nadeau ◽  
Steve J. Charette ◽  
Jacques Landry
Keyword(s):  
Oxidative Stress ◽  
Disulfide Bonds ◽  
Reductase Activity ◽  
The Other ◽  
Activation Loop ◽  
Cysteine Oxidation ◽  
Jnk Pathway ◽  
Induction Of Apoptosis ◽  
Thiol Reductase ◽  
Jnk Activation

ASK1 cysteine oxidation allows JNK activation upon oxidative stress. Trx1 negatively regulates this pathway by reducing the oxidized cysteines of ASK1. However, precisely how oxidized ASK1 is involved in JNK activation and how Trx1 regulates ASK1 oxidoreduction remains elusive. Here, we describe two different thiol reductase activities of Trx1 on ASK1. First, in H2O2-treated cells, Trx1 reduces the various disulfide bonds generated between cysteines of ASK1 by a rapid and transient action. Second, in untreated cells, Trx1 shows a more stable thiol reductase activity on cysteine 250 (Cys250) of ASK1. After H2O2 treatment, Trx1 dissociates from Cys250, which is not sufficient to activate the ASK1-JNK pathway. Indeed, in untreated cells, a Cys250 to alanine mutant of ASK1 (C250A), which cannot bind Trx1, does not constitutively activate JNK. On the other hand, in H2O2-treated cells, this mutant (C250A) fails to activate JNK and does not induce apoptosis, although it remains fully phosphorylated on Threonine 838 (Thr838) in its activation loop. Overall, our data show that Cys250 is essential for H2O2-dependent signaling downstream from ASK1 but at a step subsequent to the phosphorylation of ASK1 Thr838. They also clarify the thiol reductase function of Trx1 on ASK1 activity.

Theoretical insights into the thiol reductase activity of ebtellur

2009 ◽  
Vol 109 (10) ◽  
pp. 2297-2307 ◽  
Author(s):  
Yukiko Sakimoto ◽  
Kimihiko Hirao ◽  
Djamaladdin G. Musaev
Keyword(s):  
Reductase Activity ◽  
Thiol Reductase

Hubungan Aktivitas Nitrat Reduktase dan Kadar Klorofil Kultivar Kedelai Tahan Kekeringan

Agrotek ◽  
2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Ivonne Fitri Mariay
Keyword(s):  
Drought Stress ◽  
Nitrate Reductase ◽  
Drought Tolerance ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Growth ◽  
Block Design ◽  
Soybean Cultivar ◽  
Tissue Water ◽  
Drought Tolerant

<em>Plants suffering from drought stress can be indicated by the changing of character on the morphology, anatomy and physiology, characterized by inhibition of leaf growth, accelerated root growth, stomata closure and leaf curly. Cultivars that are tolerance to drought can physiologically adapted by increasing prolin level reduce the osmotic potential in order to maintain turgor in the condition of low tissue water potential. The research was aimed to study the physiological characters of drought tolerant soybean cultivar of determine the relationship of nitrate reductase activity and chlorophyll content of drought-tolerance cultivars and susceptible to drought stress conditions. The research was conducted at Tridharma Garden Agricultural Faculty of Gadjah Mada University, Banguntapan, Bantul, Daerah Istimewa Yogyakarta from January to March 2012. The experiment was arranged on Factorial Complete Block Design with three replications. The first factor was soybean cultivar consist of five levels those are Wilis (drought tolerance), Tidar (drought tolerant), Gema (drought tolerant), Grobogan (susceptible) and Argomulyo (susceptible). The second factor was watering interval consist of four levels which were watering once a day, every 2 days, every 4 days and every 8 days. Drought stress treatment begans at 24 DAP. Parameters observed were moisture content, nitrate reductase activity, chlorophyll a, chlorophyll b, and total of chlorophyll at 56 DAP. Portable data analysis using SAS 9.1. for windows. The results showed that nitrate reductase activity contributes to greater levels of chlorophyll of drought tolerance cultivars "Wilis", "Tidar" and "Gema" on the conditions of drought stress than the susceptible cultivar "Grobogan" and "Argomulyo".</em>

scholarly journals PHYSIOLOGICAL CHARACTERIZATION OF SIX WHEAT GENOTYPES FOR DROUGHT TOLERANCE

2016 ◽  
Vol 4 (2) ◽  
pp. 184-196
Author(s):  
HanifChachar Muhammad ◽  
Nazir Ahmed Chachar ◽  
Qamaruddin Chachar ◽  
Mujtaba Sheikh Muhammad ◽  
Sadaruddin Chachar ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Tolerance ◽  
Osmotic Potential ◽  
Glycine Betaine ◽  
Global Climate ◽  
Reductase Activity ◽  
Current Data ◽  
Wheat Genotypes ◽  
Physiological Characterization ◽  
Terminal Drought

Pakistan is one of the most severely affected countries by Global climate change, it is an agriculture based country and its economy (21%) mainly depend on agriculture production. Wheat is the major staple food crop in Pakistan and takes key position in the national economy. It contribute 12.5% share in agriculture and 2.9% in the country’s GDP. Frequent droughts and scarcity of the water severely affecting the wheat production. To fulfill the feed requirements of rapidly growing population, it is necessary to explore the advanced genetic resource that can be able to perform better in changing climate. Six wheat genotypes were tested for their early seedling and physiological performance under different water stress environments. The seeds of six wheat genotypes (Khirman, Chakwal-86, MSH-36, DH-3/48, NIA Amber and NIA-10 10/8) were tested for physiological characterization under pot house experiment for individual genotypic response to water stress. The variance of analysis shows two-way interaction water stress [Control (normal four irrigations) and terminal drought (Soaking dose) and wheat genotypes (P≤ 0.05). Seven physiological indices, including Proline content, Glycine-betaine, Total sugars, Total chlorophyll, Nitrate Reductase Activity ((NRA), Potassium (K+) content, and Osmotic potential (OP) were used to evaluate the drought tolerance of six wheat genotypes. From the current data it was illustrated that, MSH-36 and DH-3/48 exhibited the tolerance followed by, Khirman and Chakwal-86 by maintaining their osmotic potential and accumulation of higher proline and glycine-betaine content that helpful for plant to enhancing their tolerance under water stress and to maintain their growth and development, whereas NIA Amber and NIA-10 10/8 are the drought sensitive genotypes as they could not maintain their osmotic potential under drought stress environment.

scholarly journals Trichinella spiralis: Knockdown of gamma interferon inducible lysosomal thiol reductase (GILT) results in the reduction of worm burden

2021 ◽  
Vol 15 (11) ◽  
pp. e0009958
Author(s):  
Hong Fei ◽  
Muhammad Ali-ul-Husnain Naqvi ◽  
Sana Zahra Naqvi ◽  
Lixin Xu ◽  
Xiaokai Song ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Trichinella Spiralis ◽  
Reductase Activity ◽  
3D Structure ◽  
Gamma Interferon ◽  
Pcr Analysis ◽  
Multiple Sequence ◽  
Small Interference Rna ◽  
Thiol Reductase

Trichinella spiralis is mammalian skeletal muscles parasite which may cause trichinellosis in animals and humans. Gamma interferon inducible lysosomal thiol reductase (GILT) is a widespread superfamily which plays key role in processing and presentation of MHC class II restricted antigen by catalyzing disulfide bond reduction. There are no reports about GILT in T. spiralis. In present study, GILT from T. spiralis (Tsp-GILT) was cloned, analyzed by multiple-sequence alignment, and predicted by 3D structure model. Recombinant Tsp-GILT (about 46 kDa) was efficiently expressed in Escherichia coli and thiol reductase activity suggested that in acidic environment the addition of a reducing agent is needed. Soaking method was used to knockdown expression of Tsp-GILT using small interference RNA (siRNA). Immunofluorescence assay confirmed the transformation of siRNA into muscle larva (ML) and new born larva (NBL). Quantitative real time-PCR (QRT-PCR) analysis revealed that transcription level of Tsp-GILT mRNA can be up-regulated by stimulation of mouse IFN-γ and down-regulated by siRNA2 in vitro. NBLs soaked with siRNA2 showed 32.3% reduction in the generation of MLs. MLs soaked with siRNA2 showed 26.2% reduction in the next generation of MLs, but no significant effect was observed on adult worms or NBLs. These findings concluded that GILT may play important roles in the development of T. spiralis parasite.

Lamin B receptor: role on chromatin structure, cellular senescence and possibly aging

2020 ◽  
Vol 477 (14) ◽  
pp. 2715-2720
Author(s):  
Susana Castro-Obregón
Keyword(s):  
Cellular Senescence ◽  
Nuclear Membrane ◽  
Chromatin Structure ◽  
Cholesterol Synthesis ◽  
Reductase Activity ◽  
Chimeric Protein ◽  
Nuclear Lamina ◽  
Lamin B ◽  
Inner Nuclear Membrane ◽  
Lamin B Receptor

The nuclear envelope is composed by an outer nuclear membrane and an inner nuclear membrane, which is underlain by the nuclear lamina that provides the nucleus with mechanical strength for maintaining structure and regulates chromatin organization for modulating gene expression and silencing. A layer of heterochromatin is beneath the nuclear lamina, attached by inner nuclear membrane integral proteins such as Lamin B receptor (LBR). LBR is a chimeric protein, having also a sterol reductase activity with which it contributes to cholesterol synthesis. Lukasova et al. showed that when DNA is damaged by ɣ-radiation in cancer cells, LBR is lost causing chromatin structure changes and promoting cellular senescence. Cellular senescence is characterized by terminal cell cycle arrest and the expression and secretion of various growth factors, cytokines, metalloproteinases, etc., collectively known as senescence-associated secretory phenotype (SASP) that cause chronic inflammation and tumor progression when they persist in the tissue. Therefore, it is fundamental to understand the molecular basis for senescence establishment, maintenance and the regulation of SASP. The work of Lukasova et al. contributed to our understanding of cellular senescence establishment and provided the basis that lead to the further discovery that chromatin changes caused by LBR reduction induce an up-regulated expression of SASP factors. LBR dysfunction has relevance in several diseases and possibly in physiological aging. The potential bifunctional role of LBR on cellular senescence establishment, namely its role in chromatin structure together with its enzymatic activity contributing to cholesterol synthesis, provide a new target to develop potential anti-aging therapies.

Sign in / Sign up

Export Citation Format

Share Document