scholarly journals Expression of the vault RNA protects cells from undergoing apoptosis

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Melanie Amort ◽  
Birgit Nachbauer ◽  
Selma Tuzlak ◽  
Arnd Kieser ◽  
Aloys Schepers ◽  
...  
Keyword(s):  
Vault Rna

scholarly journals The human vault RNA enhances tumorigenesis and chemoresistance through the lysosome in hepatocellular carcinoma

Autophagy ◽  
2021 ◽  
pp. 1-13
Author(s):  
Iolanda Ferro ◽  
Jacopo Gavini ◽  
Stefano Gallo ◽  
Lisamaria Bracher ◽  
Marc Landolfo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Vault Rna

scholarly journals The human vault RNA enhances tumorigenesis and chemoresistance through the lysosome

2020 ◽  
Author(s):  
Iolanda Ferro ◽  
Jacopo Gavini ◽  
Lisamaria Bracher ◽  
Marc Landolfo ◽  
Daniel Candinas ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
Chemotherapy Resistance ◽  
Autophagic Flux ◽  
Tumor Cell Proliferation ◽  
Apoptosis Resistance ◽  
Knock Out ◽  
Vault Rna

AbstractThe small non-coding vault RNA (vtRNA) 1-1 has been shown to confer apoptosis resistance in several malignant cell lines and also to modulate the autophagic flux in hepatocytes, thus highlighting its pro-survival role. Here we describe a new function of vtRNA1-1 in regulating in vitro and in vivo tumor cell proliferation, tumorigenesis and chemoresistance. By activating extracellular signal-regulated kinases (ERK 1/2), vtRNA1-1 knock-out (KO) inhibits transcription factor EB (TFEB), leading to a downregulation of the coordinated lysosomal expression and regulation (CLEAR) network genes and lysosomal compartment dysfunction. Pro-tumorigenic pathways dysregulation and decreased lysosome functionality potentiate the anticancer effect of conventional targeted cancer drugs in the absence of vtRNA1-1. Finally, vtRNA1-1 KO-reduced lysosomotropism, together with a higher intracellular compound availability, significantly reduced tumor cell proliferation in vitro and in vivo. These findings reveal the role of vtRNA1-1 in ensuring intracellular catabolic compartment stability and functionality, suggesting its importance in lysosome-mediated chemotherapy resistance.

Identification of conserved vault RNA expression elements and a non-expressed mouse vault RNA gene

Gene ◽  
2003 ◽  
Vol 309 (2) ◽  
pp. 65-70 ◽  
Author(s):  
Valerie A. Kickhoefer ◽  
Nil Emre ◽  
Andrew G. Stephen ◽  
Michael J. Poderycki ◽  
Leonard H. Rome
Keyword(s):  
Rna Expression ◽  
Vault Rna

Epstein–Barr Virus-Induced Expression of a Novel Human Vault RNA

2009 ◽  
Vol 388 (4) ◽  
pp. 776-784 ◽  
Author(s):  
Constanze Nandy ◽  
Jan Mrázek ◽  
Heribert Stoiber ◽  
Friedrich A. Grässer ◽  
Alexander Hüttenhofer ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Induced Expression ◽  
Barr Virus ◽  
Epstein Barr ◽  
Vault Rna

scholarly journals The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle

2001 ◽  
Vol 152 (1) ◽  
pp. 157-164 ◽  
Author(s):  
Valerie A. Kickhoefer ◽  
Yie Liu ◽  
Lawrence B. Kong ◽  
Bryan E. Snow ◽  
Phoebe L. Stewart ◽  
...  
Keyword(s):  
Rna Stability ◽  
Three Dimensional ◽  
Telomerase Activity ◽  
Telomerase Rna ◽  
Protein Subunit ◽  
Negative Stain ◽  
Dimensional Reconstruction ◽  
Stable Association ◽  
Vault Rna

Vaults and telomerase are ribonucleoprotein (RNP) particles that share a common protein subunit, TEP1. Although its role in either complex has not yet been defined, TEP1 has been shown to interact with the mouse telomerase RNA and with several of the human vault RNAs in a yeast three-hybrid assay. An mTep1−/− mouse was previously generated which resulted in no apparent change in telomere length or telomerase activity in six generations of mTep1-deficient mice. Here we show that the levels of the telomerase RNA and its association with the telomerase RNP are also unaffected in mTep1−/− mice. Although vaults purified from the livers of mTep1−/− mice appear structurally intact by both negative stain and cryoelectron microscopy, three-dimensional reconstruction of the mTep1−/− vault revealed less density in the cap than previously observed for the intact rat vault. Furthermore, the absence of TEP1 completely disrupted the stable association of the vault RNA with the purified vault particle and also resulted in a decrease in the levels and stability of the vault RNA. Therefore, we have uncovered a novel role for TEP1 in vivo as an integral vault protein important for the stabilization and recruitment of the vault RNA to the vault particle.

scholarly journals A new role for vault RNA–TEP1 complexes in mRNA production in trypanosomes

2019 ◽  
Vol 294 (43) ◽  
pp. 15575-15576 ◽  
Author(s):  
Valerie A. Kickhoefer
Keyword(s):  
Vault Rna

scholarly journals Interactions between antitumor drugs and vault RNA

2008 ◽  
Vol 52 (1) ◽  
pp. 217-218 ◽  
Author(s):  
T. Mashima ◽  
M. Kudo ◽  
Y. Takada ◽  
A. Matsugami ◽  
S. C. B. Gopinath ◽  
...  
Keyword(s):  
Antitumor Drugs ◽  
Vault Rna

scholarly journals Upregulation of a small vault RNA (svtRNA2-1a) is an early event in Parkinson disease and induces neuronal dysfunction

RNA Biology ◽  
2013 ◽  
Vol 10 (7) ◽  
pp. 1093-1106 ◽  
Author(s):  
Elena Miñones-Moyano ◽  
Marc R. Friedländer ◽  
Joan Pallares ◽  
Birgit Kagerbauer ◽  
Sílvia Porta ◽  
...  
Keyword(s):  
Parkinson Disease ◽  
Early Event ◽  
Neuronal Dysfunction ◽  
Vault Rna
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