scholarly journals Phenotypic characterization of missense polymerase-δ mutations using an inducible protein-replacement system

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Medini Manohar Ghodgaonkar ◽  
Patrick Kehl ◽  
Ilenia Ventura ◽  
Liyan Hu ◽  
Margherita Bignami ◽  
...  
Keyword(s):  
Phenotypic Characterization ◽  
Replacement System ◽  
Inducible Protein

scholarly journals Functional and phenotypic characterization of a protein from Lactobacillus acidophilus involved in cell morphology, stress tolerance and adherence to intestinal cells

Microbiology ◽  
2010 ◽  
Vol 156 (11) ◽  
pp. 3360-3367 ◽  
Author(s):  
Sarah J. O'Flaherty ◽  
Todd R. Klaenhammer
Keyword(s):  
Cell Surface ◽  
Stress Tolerance ◽  
Cell Morphology ◽  
Surface Protein ◽  
Gene Replacement ◽  
Probiotic Bacteria ◽  
Phenotypic Characterization ◽  
Replacement System

Structural components of the cell surface have an impact on some of the beneficial attributes of probiotic bacteria. In silico analysis of the L. acidophilus NCFM genome sequence revealed the presence of a putative cell surface protein that was predicted to be a myosin cross-reactive antigen (MCRA). As MCRAs are conserved among many probiotic bacteria, we used the upp-based counterselective gene replacement system, designed recently for use in L. acidophilus, to determine the functional role of this gene (LBA649) in L. acidophilus NCFM. Phenotypic assays were undertaken with the parent strain (NCK1909) and deletion mutant (NCK2015) to assign a function for this gene. The growth of NCK2015 (ΔLBA649) was reduced in the presence of lactate, acetate, porcine bile and salt. Adhesion of NCK2015 to Caco-2 cells was substantially reduced for both stationary-phase (∼45 % reduction) and exponential-phase cells (∼50 % reduction). Analysis of NCK2015 by scanning electron microscopy revealed a longer cell morphology after growth in MRS broth compared to NCK1909. These results indicate a role for LBA649 in stress tolerance, cell wall division and adherence to Caco-2 cells.

Comprehensive phenotypic characterization of ABCB4 deficient mice reveals systemic effects of spontaneous biliary fibrosis

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
K Hochrath ◽  
S Hillebrandt ◽  
F Lammert ◽  
B Rathkolb ◽  
H Fuchs ◽  
...  
Keyword(s):  
Phenotypic Characterization ◽  
Systemic Effects ◽  
Deficient Mice

Phenotypic Characterization of a Family With An In-frame Deletion in the DMD Gene and Variable Penetrance

2018 ◽  
Vol 18 (4) ◽  
pp. 246-251 ◽  
Author(s):  
Inmaculada Perez-Sanchez ◽  
Maria Sabater-Molina ◽  
Maria Elisa Nicolas Rocamora ◽  
Guillermo Glover ◽  
Fuensanta Escudero ◽  
...  
Keyword(s):  
Phenotypic Characterization ◽  
Dmd Gene ◽  
Variable Penetrance

scholarly journals Towards Development, Maintenance, and Standardized Phenotypic Characterization of Single‐Seed‐Descent Genetic Resources for Lupins

2021 ◽  
Vol 1 (7) ◽  
Author(s):  
Magdalena Kroc ◽  
Magdalena Tomaszewska ◽  
Katarzyna Czepiel ◽  
Elena Bitocchi ◽  
Markus Oppermann ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Phenotypic Characterization ◽  
Single Seed Descent ◽  
Single Seed

Biochemical and phenotypic characterization of sweet cherry (Prunus avium L.) cultivars with induced surface pitting

2021 ◽  
Vol 175 ◽  
pp. 111494
Author(s):  
Excequel Ponce ◽  
Blanca Alzola ◽  
Natalia Cáceres ◽  
Madeline Gas ◽  
Catalina Ferreira ◽  
...  
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Phenotypic Characterization ◽  
Prunus Avium L
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