scholarly journals Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Chenhui Hao ◽  
Xiang Li ◽  
Cheng Tian ◽  
Wen Jiang ◽  
Guansong Wang ◽  
...  
Keyword(s):  
Packaging Rna

Packaging RNA drugs into extracellular vesicles

2020 ◽  
Vol 4 (1) ◽  
pp. 6-8
Author(s):  
Monique A. J. van Eijndhoven ◽  
S. Rubina Baglio ◽  
D. Michiel Pegtel
Keyword(s):  
Extracellular Vesicles ◽  
Packaging Rna

scholarly journals Chemical Modification Patterns of Active and Inactive as Well as Procapsid-Bound and Unbound DNA-Packaging RNA of Bacterial Virus Phi29

Virology ◽  
2001 ◽  
Vol 281 (2) ◽  
pp. 281-293 ◽  
Author(s):  
Chunlin Zhang ◽  
Mark Trottier ◽  
Chaoping Chen ◽  
Peixuan Guo
Keyword(s):  
Chemical Modification ◽  
Dna Packaging ◽  
Bacterial Virus ◽  
Packaging Rna

scholarly journals RNAs Extracted from Herpes Simplex Virus 1 Virions: Apparent Selectivity of Viral but Not Cellular RNAs Packaged in Virions

2001 ◽  
Vol 75 (17) ◽  
pp. 8105-8116 ◽  
Author(s):  
Maria-Teresa Sciortino ◽  
Mikiko Suzuki ◽  
Brunella Taddeo ◽  
Bernard Roizman
Keyword(s):  
Reverse Transcription ◽  
Rna Binding ◽  
Cdna Array ◽  
Open Reading Frames ◽  
Herpes Simplex Virus 1 ◽  
Cdna Arrays ◽  
Series Of Experiments ◽  
Packaging Rna ◽  
Simplex Virus

ABSTRACT Following the lead of recent studies on the presence of RNA in virions of human cytomegalovirus, we investigated the presence and identity of RNAs from purified virions of herpes simple virus 1. To facilitate these studies, we designed primers for all known open reading frames (ORFs) and also constructed cDNA arrays containing probes designed to detect all known transcripts. In the first series of experiments, labeled DNA made by reverse transcription of poly(A)+ RNA extracted from infected HEp-2 or rabbit skin cells hybridized to all but two of the probes in the cDNA array. A similar analysis of the RNA extracted from purified extracellular virions derived from infected HEp-2 cells hybridized to probes representing 24 of the ORFs. In the second series of analyses, we reverse transcribed and amplified by PCR RNAs from purified intracellular or extracellular virions derived from infected HEp-2 or Vero cell lines. The positive RNAs were retested by PCR with and without prior reverse transcription to ensure that the samples tested were free of contaminating DNA. The results were as follows. (i) Only a fraction of viral ORF transcripts were represented in virion RNA, and only nine RNAs (UL10, UL34/UL35, UL36, UL42, UL48, UL51, US1/US1.5, US8.5, and US10/US11) were positive in all RT PCR assays. Of these, seven were positive by hybridization to cDNA arrays. (ii) RNA extracted from cells infected with a mutant virus lacking the US8 to US12 genes yielded results similar to those described above, indicating that US11, a known RNA binding protein, does not play a role in packaging RNA in virions. (iii) Cellular RNAs detected in virions were representative of the abundant cellular RNAs. Last, RNA extracted from virions was translated in vitro and the translation products were reacted with antibody to αTIF (VIP16). The immune precipitate contained a labeled protein with the apparent molcular weight of αTIF, indicating that at least one mRNA packaged in virions was intact and capable of being translated. The basis for the apparent selectivity in the packaging of the viral RNAs packaged in virions is unknown.

scholarly journals Development of Targeted Therapy Therapeutics to Sensitize Triple-Negative Breast Cancer Chemosensitivity Utilizing Bacteriophage phi29 Derived Packaging RNA

2020 ◽  
Author(s):  
Long Zhang ◽  
Chaofeng Mu ◽  
Tinghong Zhang ◽  
Dejun Yang ◽  
Luhui Fan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Targeted Therapy ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Treatment Options ◽  
Sirna Delivery ◽  
Growth Factor Receptor ◽  
Her2 Breast Cancer ◽  
Packaging Rna

Abstract Background: To date, triple-negative breast cancer (TNBC) treatment options are limited due to it lacks expression of receptors and are only available managed with chemotherapy. What's worse, TNBC is frequently developing resistance to chemotherapy. By using siRNA-based therapeutics, our recent work demonstrated X-box-binding protein 1 (XBP1) was linked to HER2+ breast cancer development and chemoresistance. As is well-known, the instability, off-target effects, net negative charge, and hydrophobicity of siRNA hamper its’ in vivo utilization and clinical transformation. Thus, the development of a siRNA delivery system (DDS) with ultra-stability and specificity is demanded to address the predicament of siRNA delivery.Results: Here, we assembled RNase resistant RNA nanoparticles (NPs) based on the 3WJ of Phi29 DNA packaging motor. To targeted therapy and sensitize TNBC to chemotherapy, the RNA NPs were equipped with epidermal growth factor receptor (EGFR) targeting aptamer and XBP1 siRNA. We found our RNA NPs could deplete XBP1 expression and suppress tumor growth after intravenous administration. Meanwhile, RNA NPs treatment could promote the sensitization of chemotherapy and impair angiogenesis in vivo. Conclusions: The results further demonstrate that our RNA NPs could serve as an effective and promising platform not only for siRNA delivery but also for chemotherapy-resistant TNBC therapy.

scholarly journals Thermodynamic Stabilities of Multibranch Loops in Bacteriophage Packaging RNA

2016 ◽  
Vol 110 (3) ◽  
pp. 410a
Author(s):  
Alyssa Hill ◽  
Susan Schroeder
Keyword(s):  
Packaging Rna ◽  
Multibranch Loops

scholarly journals Global Structure of a Three-Way Junction in a Phi29 Packaging RNA Dimer Determined Using Site-Directed Spin Labeling

2012 ◽  
Vol 134 (5) ◽  
pp. 2644-2652 ◽  
Author(s):  
Xiaojun Zhang ◽  
Chang-Shung Tung ◽  
Glenna Z. Sowa ◽  
Ma’mon M. Hatmal ◽  
Ian S. Haworth ◽  
...  
Keyword(s):  
Spin Labeling ◽  
Global Structure ◽  
Rna Dimer ◽  
Site Directed Spin Labeling ◽  
Packaging Rna

scholarly journals Mapping the Global Conformation of the Phi29 Packaging RNA Dimer Using Deer Distance Constraints

2010 ◽  
Vol 98 (3) ◽  
pp. 264a
Author(s):  
Xiaojun Zhang ◽  
Mamoon Hatmal ◽  
Ian Haworth ◽  
Peter Z. Qin
Keyword(s):  
Distance Constraints ◽  
Rna Dimer ◽  
Packaging Rna
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