scholarly journals Horizontal gene transfer converts non-toxigenic Clostridium difficile strains into toxin producers

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Michael S.M. Brouwer ◽  
Adam P. Roberts ◽  
Haitham Hussain ◽  
Rachel J. Williams ◽  
Elaine Allan ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Clostridium Difficile ◽  
Horizontal Gene Transfer ◽  
Toxin Producers

scholarly journals Demonstration of Conjugative Transposon (Tn5397)-Mediated Horizontal Gene Transfer between Clostridium difficile and Enterococcus faecalis

2010 ◽  
Vol 54 (11) ◽  
pp. 4924-4926 ◽  
Author(s):  
Azmiza S. Jasni ◽  
Peter Mullany ◽  
Haitham Hussain ◽  
Adam P. Roberts
Keyword(s):  
Gene Transfer ◽  
Clostridium Difficile ◽  
Horizontal Gene Transfer ◽  
Enterococcus Faecalis ◽  
Nosocomial Infections ◽  
Conjugative Transposon ◽  
Antibiotic Resistant ◽  
Specific Target ◽  
Ribotype 027 ◽  
Target Sites

ABSTRACT Antibiotic-resistant Enterococcus faecalis and Clostridium difficile are responsible for nosocomial infections in humans, in which they inhabit the same niche. Here, we demonstrate transfer of the conjugative transposon Tn5397 from C. difficile 630 to E. faecalis JH2-2, the first reported gene transfer between these two bacteria. Furthermore, transfer from the E. faecalis EF20A transconjugant to the epidemic ribotype 027 C. difficile strain R20291 was also demonstrated. Tn5397 was shown to use a single specific target site in E. faecalis; it also has specific target sites in C. difficile. These experiments highlight the importance of continual monitoring for emerging resistances in these bacteria.

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