scholarly journals Periodic Wnt1 expression in response to ecdysteroid generates twin-spot markings on caterpillars

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Junichi Yamaguchi ◽  
Yutaka Banno ◽  
Kazuei Mita ◽  
Kimiko Yamamoto ◽  
Toshiya Ando ◽  
...  
Keyword(s):  
Wnt1 Expression

Zebrafish aussicht mutant embryos exhibit widespread overexpression of ace (fgf8) and coincident defects in CNS development

Development ◽  
1999 ◽  
Vol 126 (10) ◽  
pp. 2129-2140 ◽  
Author(s):  
C.P. Heisenberg ◽  
C. Brennan ◽  
S.W. Wilson
Keyword(s):  
Nervous System ◽  
Neural Retina ◽  
Cns Development ◽  
Dominant Mutation ◽  
Ace Gene ◽  
Retinal Epithelium ◽  
Ace Activity ◽  
Pretectal Area ◽  
Wnt1 Expression

During the development of the zebrafish nervous system both noi, a zebrafish pax2 homolog, and ace, a zebrafish fgf8 homolog, are required for development of the midbrain and cerebellum. Here we describe a dominant mutation, aussicht (aus), in which the expression of noi and ace is upregulated. In aus mutant embryos, ace is upregulated at many sites in the embryo, while noi expression is only upregulated in regions of the forebrain and midbrain which also express ace. Subsequent to the alterations in noi and ace expression, aus mutants exhibit defects in the differentiation of the forebrain, midbrain and eyes. Within the forebrain, the formation of the anterior and postoptic commissures is delayed and the expression of markers within the pretectal area is reduced. Within the midbrain, En and wnt1 expression is expanded. In heterozygous aus embryos, there is ectopic outgrowth of neural retina in the temporal half of the eyes, whereas in putative homozygous aus embryos, the ventral retina is reduced and the pigmented retinal epithelium is expanded towards the midline. The observation that aus mutant embryos exhibit widespread upregulation of ace raised the possibility that aus might represent an allele of the ace gene itself. However, by crossing carriers for both aus and ace, we were able to generate homozygous ace mutant embryos that also exhibited the aus phenotype. This indicated that aus is not tightly linked to ace and is unlikely to be a mutation directly affecting the ace locus. However, increased Ace activity may underly many aspects of the aus phenotype and we show that the upregulation of noi in the forebrain of aus mutants is partially dependent upon functional Ace activity. Conversely, increased ace expression in the forebrain of aus mutants is not dependent upon functional Noi activity. We conclude that aus represents a mutation involving a locus normally required for the regulation of ace expression during embryogenesis.

scholarly journals Long noncoding RNA DLGAP1-AS2 facilitates Wnt1 transcription through physically interacting with Six3 and drives the malignancy of gastric cancer

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jiawei Lu ◽  
Ying Xu ◽  
Wenjie Xie ◽  
Yinbing Tang ◽  
Heteng Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Transcriptional Activation ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Migration And Invasion ◽  
Promoter Regions ◽  
Systematic Analysis ◽  
Downstream Target ◽  
Signaling Axis ◽  
Wnt1 Expression

AbstractThe long noncoding RNA (lncRNA) DLGAP1-AS2 has recently been characterized as an oncogenic lncRNA in several cancers. However, its biological roles and clinical significance in gastric cancer (GC) remains barely understood. In this study, we performed a systematic analysis of DLGAP1-AS2 expression with data from the TCGA and GEO database as well as our clinic GC samples. In agreement with previous studies, our findings demonstrated that DLGAP1-AS2 was significantly up-regulated in GC and its high expression was associated with poor prognosis, suggesting that DLGAP1-AS2 might be a putative oncogenic lncRNA of GC. Loss of DLGAP1-AS2 restricted cell proliferation, migration, and invasion in GC cell lines. Mechanically, Wnt1 was identified as the downstream target of DLGAP1-AS2 by using bioinformatics analysis coupled with qPCR and Western blot assays. Furthermore, DLGAP1-AS2 was found to directly interact with the transcriptional repressor Six3, and this interaction hampered Six3 binding to the promoter regions of the Wnt1 gene, thereby leading to transcriptional activation of Wnt1. Consequently, GC cells lacking DLGAP1-AS2 showed a decreased Wnt1 expression and weakened Wnt/β-catenin signaling. Further, Six3 silencing could reverse the above effects, highlighting a pivotal role of Six3 in the DLGAP1-AS2-mediated activation of Wnt/β-catenin signaling. Either genetic (Wnt1 knockdown) or pharmacological (LF3) inhibition of Wnt/β-catenin signaling could effectively abolish the activation of Wnt/β-catenin signaling by Six3 depletion, thereby preventing GC cell malignant transformation. Taken together, our results suggest that DLGAP1-AS2 functions as an oncogenic factor by directly interacting with Six3 to relieve its suppression on Wnt1 expression, thereby driving the malignancy of GC. DLGAP1-AS2/Six3/Wnt1/β-catenin signaling axis might serve as a promising diagnostic and therapeutic target for GC.

scholarly journals spiel ohne grenzen/pou2is required during establishment of the zebrafish midbrain-hindbrain boundary organizer

Development ◽  
2001 ◽  
Vol 128 (21) ◽  
pp. 4165-4176 ◽  
Author(s):  
Heinz-Georg Belting ◽  
Giselbert Hauptmann ◽  
Dirk Meyer ◽  
Salim Abdelilah-Seyfried ◽  
Ajay Chitnis ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Ectopic Expression ◽  
Regional Identity ◽  
Positional Information ◽  
Neural Plate ◽  
Pou Domain ◽  
Normal Expression ◽  
Organizing Center ◽  
Wnt1 Expression ◽  
Permissive Role

The vertebrate midbrain-hindbrain boundary (MHB) organizes patterning and neuronal differentiation in the midbrain and anterior hindbrain. Formation of this organizing center involves multiple steps, including positioning of the MHB within the neural plate, establishment of the organizer and maintenance of its regional identity and signaling activities. Juxtaposition of the Otx2 and Gbx2 expression domains positions the MHB. How the positional information is translated into activation of Pax2, Wnt1 and Fgf8 expression during MHB establishment remains unclear. In zebrafish spiel ohne grenzen (spg) mutants, the MHB is not established, neither isthmus nor cerebellum form, the midbrain is reduced in size and patterning abnormalities develop within the hindbrain. In spg mutants, despite apparently normal expression of otx2, gbx1 and fgf8 during late gastrula stages, the initial expression of pax2.1, wnt1 and eng2, as well as later expression of fgf8 in the MHB primordium are reduced. We show that spg mutants have lesions in pou2, which encodes a POU-domain transcription factor. Maternal pou2 transcripts are distributed evenly in the blastula, and zygotic expression domains include the midbrain and hindbrain primordia during late gastrulation. Microinjection of pou2 mRNA can rescue pax2.1 and wnt1 expression in the MHB of spg/pou2 mutants without inducing ectopic expression. This indicates an essential but permissive role for pou2 during MHB establishment. pou2 is expressed normally in noi/pax2.1 and ace/fgf8 zebrafish mutants, which also form no MHB. Thus, expression of pou2 does not depend on fgf8 and pax2.1. Our data suggest that pou2 is required for the establishment of the normal expression domains of wnt1 and pax2.1 in the MHB primordium.

scholarly journals Protruding Structures on Caterpillars Are Controlled by Ectopic Wnt1 Expression

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0121736 ◽  
Author(s):  
Mina Edayoshi ◽  
Junichi Yamaguchi ◽  
Haruhiko Fujiwara
Keyword(s):  
Wnt1 Expression

Identification of connexin43 as a functional target for Wnt signalling

1998 ◽  
Vol 111 (12) ◽  
pp. 1741-1749 ◽  
Author(s):  
M.A. van der Heyden ◽  
M.B. Rook ◽  
M.M. Hermans ◽  
G. Rijksen ◽  
J. Boonstra ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Target Genes ◽  
Wnt Signalling ◽  
Epithelial Cell Line ◽  
Transcriptional Level ◽  
Cx43 Expression ◽  
Gap Junctional Communication ◽  
Junctional Communication ◽  
Gap Junctional ◽  
Wnt1 Expression

Wnt mediated signal transduction is considered to regulate activity of target genes. In Xenopus embryos, ectopic Wnt1 and Wnt8 expression induces gap-junctional communication. During murine brain formation, Wnt1 and the gap-junctional protein connexin43 (Cx43) are co-expressed at the mid/hindbrain border, while interference with Wnt1 or Cx43 expression during embryogenesis leads to severe brain defects in the mid/hindbrain region. In PC12 cells, Wnt1 expression leads to an apparent increase in cell-cell adhesion. We investigated the effects of Wnt1 overexpression on gap-junctional communication in PC12 cells. Wnt1 expressing clones displayed an increased electrical and chemical coupling. This coincides with an increased expression of Cx43 mRNA and protein, while other connexins, Cx26, Cx32, Cx37, Cx40 and Cx45, were not up-regulated. Also, induction of Wnt1 expression in a mammary epithelial cell line leads to an increase in gap-junctional communication and Cx43 protein expression. In transient transactivation assays in P19 EC cells we found that Wnt1 and Li+, an ion that mimics Wnt signalling, increased transcription from the rat Cx43 promoter, potentially via TCF/LEF binding elements, in a pathway separate from cAMP-induced Cx43 transactivation. The results demonstrate that Cx43 acts as a functional target of Wnt1 signalling, and Cx43 expression can be regulated by Wnt1 at the transcriptional level. Our data suggest that Wnt1-induced cell fate determination is likely to involve regulation of gap-junctional communication.

New insights into opioid regulatory pathways: influence of opioids on Wnt1 expression in zebrafish embryos

Neuroscience ◽  
2012 ◽  
Vol 200 ◽  
pp. 237-247 ◽  
Author(s):  
F.M. Sanchez-Simon ◽  
A.S. Ledo ◽  
R. Arevalo ◽  
R.E. Rodriguez
Keyword(s):  
Zebrafish Embryos ◽  
Regulatory Pathways ◽  
Wnt1 Expression
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