scholarly journals VE-PTP regulates VEGFR2 activity in stalk cells to establish endothelial cell polarity and lumen formation

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Makoto Hayashi ◽  
Arindam Majumdar ◽  
Xiujuan Li ◽  
Jeremy Adler ◽  
Zuyue Sun ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Polarity ◽  
Lumen Formation

scholarly journals β1 Integrin Establishes Endothelial Cell Polarity and Arteriolar Lumen Formation via a Par3-Dependent Mechanism

2010 ◽  
Vol 18 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Ann C. Zovein ◽  
Alfonso Luque ◽  
Kirsten A. Turlo ◽  
Jennifer J. Hofmann ◽  
Kathleen M. Yee ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Polarity ◽  
Β1 Integrin ◽  
Lumen Formation ◽  
Dependent Mechanism

scholarly journals Vinculin controls endothelial cell junction dynamics during vascular lumen formation

2021 ◽  
Author(s):  
Maria P. Kotini ◽  
Miesje M. van der Stoel ◽  
Mitchell K. Han ◽  
Bettina Kirchmaier ◽  
Johan de Rooij ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Junctions ◽  
Cell Junction ◽  
Vascular Integrity ◽  
Lumen Formation ◽  
Cell Behaviors ◽  
Sprouting Angiogenesis ◽  
Dynamic Cell ◽  
External Forces ◽  
Cell Cell

AbstractBlood vessel morphogenesis is driven by coordinated endothelial cell behaviors, which depend on dynamic cell-cell interactions. Remodeling of endothelial cell-cell junctions promote morphogenetic cellular events while preserving vascular integrity. Here, we have analyzed the dynamics of endothelial cell-cell junctions during lumen formation in angiogenic sprouts. By live-imaging of the formation of intersegmental blood vessels in zebrafish, we demonstrate that lumen expansion is accompanied by the formation of transient finger-shaped junctions. Formation and maintenance of these junctional fingers are positively regulated by blood pressure whereas inhibition of blood flow prevents their formation. Using fluorescent reporters, we show that the tension-sensor Vinculin localizes to junctional fingers. Furthermore, loss of vinculin function, in vinculin a and -b double knockouts, prevents junctional finger formation in angiogenic sprouts, whereas endothelial expression of a vinculin transgene is sufficient to restore junctional fingers. Taken together, our findings suggest a mechanism in which lumen expansion during angiogenesis leads to an increase in junctional tension, which triggers recruitment of vinculin and formation of junctional fingers. We propose that endothelial cells may employ force-dependent junctional remodeling to react to changes in external forces to protect cell-cell contacts and to maintain vascular integrity during sprouting angiogenesis.

scholarly journals Atypical cadherin FAT4 orchestrates lymphatic endothelial cell polarity in response to flow

2020 ◽  
Vol 130 (6) ◽  
pp. 3315-3328 ◽  
Author(s):  
Kelly L. Betterman ◽  
Drew L. Sutton ◽  
Genevieve A. Secker ◽  
Jan Kazenwadel ◽  
Anna Oszmiana ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Polarity ◽  
Lymphatic Endothelial Cell

scholarly journals Special Issue on “The Tight Junction and Its Proteins: More than Just a Barrier”

2020 ◽  
Vol 21 (13) ◽  
pp. 4612
Author(s):  
Susanne M. Krug ◽  
Michael Fromm
Keyword(s):  
Endothelial Cell ◽  
Tight Junction ◽  
Cell Polarity ◽  
Immune Cells ◽  
Therapeutic Target ◽  
Special Issue ◽  
Cell Sheets ◽  
Cellular Processes ◽  
Long Time ◽  
Surprising Finding

For a long time, the tight junction (TJ) was known to form and regulate the paracellular barrier between epithelia and endothelial cell sheets. Starting shortly after the discovery of the proteins forming the TJ—mainly, the two families of claudins and TAMPs—several other functions have been discovered, a striking one being the surprising finding that some claudins form paracellular channels for small ions and/or water. This Special Issue covers numerous dedicated topics including pathogens affecting the TJ barrier, TJ regulation via immune cells, the TJ as a therapeutic target, TJ and cell polarity, the function of and regulation by proteins of the tricellular TJ, the TJ as a regulator of cellular processes, organ- and tissue-specific functions, TJs as sensors and reactors to environmental conditions, and last, but not least, TJ proteins and cancer. It is not surprising that due to this diversity of topics and functions, the still-young field of TJ research is growing fast. This Editorial gives an introduction to all 43 papers of the Special Issue in a structured topical order.

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