scholarly journals β-lactam antibiotics promote bacterial mutagenesis via an RpoS-mediated reduction in replication fidelity

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
A. Gutierrez ◽  
L. Laureti ◽  
S. Crussard ◽  
H. Abida ◽  
A. Rodríguez-Rojas ◽  
...  
Keyword(s):  
Replication Fidelity ◽  
Bacterial Mutagenesis ◽  
Mediated Reduction

A Selective, Copper-Mediated Reduction in the Anti Xa Activity of Heparin

1991 ◽  
Vol 66 (06) ◽  
pp. 742-742 ◽  
Author(s):  
Zhengchun Liu ◽  
Arthur S Perlin
Keyword(s):  
Mediated Reduction

scholarly journals Impact of supervised beego, a traditional Chinese water-only fasting, on thrombosis and haemostasis

2021 ◽  
pp. bmjnph-2020-000183
Author(s):  
Yixuan Fang ◽  
Yue Gu ◽  
Chen Zhao ◽  
Yaqi Lv ◽  
Jiawei Qian ◽  
...  
Keyword(s):  
Biological Effects ◽  
Selective Reduction ◽  
Coagulation Factors ◽  
Normal Diet ◽  
Non Invasive ◽  
Cholesterol Levels ◽  
Thrombosis Risk ◽  
Abdominal Breathing ◽  
Mediated Reduction ◽  
Platelet Formation

Beego is a traditional Chinese complete water-only fasting practice initially developed for spiritual purposes, later extending to physical fitness purposes. Beego notably includes a psychological induction component that includes meditation and abdominal breathing, light body exercise and ends with a specific gradual refeeding program before returning to a normal diet. Beego has regained its popularity in recent decades in China as a strategy for helping people in subhealthy conditions or with metabolic syndrome, but we are unaware of any studies examining the biological effects of this practice. To address this, we here performed a longitudinal study of beego comprising fasting (7 and 14 day cohorts) and a 7-day programmed refeeding phase. In addition to detecting improvements in cardiovascular physiology and selective reduction of blood pressure in hypertensive subjects, we observed that beego decreased blood triacylglycerol (TG) selectively in TG-high subjects and increased cholesterol in all subjects during fasting; however, the cholesterol levels were normalised after completion of the refeeding program. Strikingly, beego reduced platelet formation, activation, aggregation and degranulation, resulting in an alleviated thrombosis risk, yet maintained haemostasis by sustaining levels of coagulation factors and other haemostatic proteins. Mechanistically, we speculate that downregulation of G6B and MYL9 may influence the observed beego-mediated reduction in platelets. Fundamentally, our study supports that supervised beego reduces thrombosis risk without compromising haemostasis capacity. Moreover, our results support that beego under medical supervision can be implemented as non-invasive intervention for reducing thrombosis risk, and suggest several lines of intriguing inquiry for future studies about this fasting practice (http://www.chictr.org.cn/index.aspx, number, ChiCTR1900027451).

Impact of Organic Matter on Microbially-Mediated Reduction and Mobilization of Arsenic and Iron in Arsenic(V)-Bearing Ferrihydrite

2020 ◽  
Author(s):  
Xiaolin Cai ◽  
Laurel K. ThomasArrigo ◽  
Xu Fang ◽  
Sylvain Bouchet ◽  
Yanshan Cui ◽  
...  
Keyword(s):  
Organic Matter ◽  
Mediated Reduction

scholarly journals Autophagy-mediated reduction of miR-345 contributes to hepatic cystogenesis in polycystic liver disease

JHEP Reports ◽  
2021 ◽  
pp. 100345
Author(s):  
Tatyana Masyuk ◽  
Anatoliy Masyuk ◽  
Christy Trussoni ◽  
Brynn Howard ◽  
Jingyi (Francess) Ding ◽  
...  
Keyword(s):  
Liver Disease ◽  
Polycystic Liver Disease ◽  
Polycystic Liver ◽  
Mediated Reduction

scholarly journals Crystal structure of steroid reductase SRD5A reveals conserved steroid reduction mechanism

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yufei Han ◽  
Qian Zhuang ◽  
Bo Sun ◽  
Wenping Lv ◽  
Sheng Wang ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Biochemical Characterization ◽  
Substrate Binding ◽  
Binding Pocket ◽  
Substrate Binding Pocket ◽  
Transmembrane Segments ◽  
Therapeutic Molecules ◽  
Binding Cavity ◽  
Immune System Regulation ◽  
Mediated Reduction

AbstractSteroid hormones are essential in stress response, immune system regulation, and reproduction in mammals. Steroids with 3-oxo-Δ4 structure, such as testosterone or progesterone, are catalyzed by steroid 5α-reductases (SRD5As) to generate their corresponding 3-oxo-5α steroids, which are essential for multiple physiological and pathological processes. SRD5A2 is already a target of clinically relevant drugs. However, the detailed mechanism of SRD5A-mediated reduction remains elusive. Here we report the crystal structure of PbSRD5A from Proteobacteria bacterium, a homolog of both SRD5A1 and SRD5A2, in complex with the cofactor NADPH at 2.0 Å resolution. PbSRD5A exists as a monomer comprised of seven transmembrane segments (TMs). The TM1-4 enclose a hydrophobic substrate binding cavity, whereas TM5-7 coordinate cofactor NADPH through extensive hydrogen bonds network. Homology-based structural models of HsSRD5A1 and -2, together with biochemical characterization, define the substrate binding pocket of SRD5As, explain the properties of disease-related mutants and provide an important framework for further understanding of the mechanism of NADPH mediated steroids 3-oxo-Δ4 reduction. Based on these analyses, the design of therapeutic molecules targeting SRD5As with improved specificity and therapeutic efficacy would be possible.

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